Acyl Chain Unsaturation and Vesicle Curvature Alter Outer Leaflet Packing and Promote Poly(ethylene glycol)-Mediated Membrane Fusion†

Biochemistry ◽  
1997 ◽  
Vol 36 (19) ◽  
pp. 5827-5836 ◽  
Author(s):  
Will A. Talbot ◽  
Lian Xing Zheng ◽  
Barry R. Lentz
Keyword(s):  
Ethylene Glycol ◽  
Membrane Fusion ◽  
Acyl Chain ◽  
Poly Ethylene Glycol ◽  
Outer Leaflet ◽  
Poly Ethylene

scholarly journals Interference of poly(ethylene glycol)–lipid analogues with cationic-lipid-mediated delivery of oligonucleotides; role of lipid exchangeability and non-lamellar transitions

2002 ◽  
Vol 366 (1) ◽  
pp. 333-341 ◽  
Author(s):  
Fuxin SHI ◽  
Luc WASUNGU ◽  
Anita NOMDEN ◽  
Marc C.A. STUART ◽  
Evgeny POLUSHKIN ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Chinese Hamster Ovary Cells ◽  
Hexagonal Phase ◽  
Acyl Chain ◽  
Chinese Hamster ◽  
Cationic Lipid ◽  
Poly Ethylene Glycol ◽  
Endosomal Compartment ◽  
Poly Ethylene

Cationic liposomes are applied to transfer oligonucleotides (ODNs) into cells to regulate gene expression for gene therapeutic or cell biological purposes. In vivo, poly(ethylene glycol) (PEG)—lipid derivatives are employed to stabilize and prolong the circulation lifetime of nucleic acid-containing particles, and to improve targeting strategies. In this study, we have studied the effects of PEG—lipid analogues, i.e. PEG coupled to either phosphatidylethanolamine (PE) or ceramide, on cationic-lipid—DNA complex ('lipoplex') assembly and the mechanism of cationic-lipid-mediated delivery of ODNs in vitro. Inclusion of 10mol% PEG—PE in ODN lipoplexes inhibited their internalization in Chinese hamster ovary cells by more than 70%. The intracellular fraction remained entrapped in the endosomal/lysosomal pathway, and no release of ODNs was apparent. Similar observations were made for complexes prepared from liposomes that contained PEG—ceramides. Interestingly, delivery resumed when lipoplexes had been externally coated with PEG—ceramides. In this case, the kinetics of delivery were dependent on the length of the ceramide acyl chain, consistent with a requirement for the PEG—lipid to dissociate from the complex. Moreover, although the chemical nature of the PEG—ceramides distinctly affected the net internalization of the complexes, impediment of delivery was largely related to an inhibitory effect of the PEG—lipid on the release of ODNs from the endosomal compartment. Cryo-electron microscopy and small-angle X-ray scattering revealed that the PEG—lipids stabilize the lamellar phase of the lipoplexes, while their acyl-chain-length-dependent transfer from the complex enables adaptation of the hexagonal phase. Within the endosomal compartment, this transition appears to be instrumental in causing the dissociation and cytosolic release of the ODNs for their nuclear homing.

scholarly journals HIV gp41 Trans-Membrane Domain Promotes both Stalk and Fusion Pore Formation in Poly(Ethylene-) Glycol Mediated Membrane Fusion

2012 ◽  
Vol 102 (3) ◽  
pp. 499a-500a
Author(s):  
Hirak Chakraborty ◽  
Sekaran Moses Dennison ◽  
David G. Klapper ◽  
Barry R. Lentz
Keyword(s):  
Ethylene Glycol ◽  
Membrane Fusion ◽  
Pore Formation ◽  
Fusion Pore ◽  
Membrane Domain ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Hiv Gp41

scholarly journals Activation Thermodynamics of Poly(Ethylene Glycol)-Mediated Model Membrane Fusion Support Mechanistic Models of Stalk and Pore Formation

2012 ◽  
Vol 102 (12) ◽  
pp. 2751-2760 ◽  
Author(s):  
Hirak Chakraborty ◽  
Pradip K. Tarafdar ◽  
Michael J. Bruno ◽  
Tanusree Sengupta ◽  
Barry R. Lentz
Keyword(s):  
Ethylene Glycol ◽  
Membrane Fusion ◽  
Pore Formation ◽  
Model Membrane ◽  
Mechanistic Models ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

PIEZOELECTRIC PROPERTIES OF POLY(3-HYDROXYBUTYRATE-CO-3-HEDROXYBUTYRATE)-CO-POLY(ETHYLENE GLYCOL) PRODUCED BY CONTROLLED MICROBIOLOGICAL BIOSYNTHESIS

2019 ◽  
Vol 10 (10) ◽  
Author(s):  
Anton Bonartsev ◽  
Vera Voinova ◽  
Elizaveta Akoulina ◽  
Andrey Dudun ◽  
Irina Zharkova ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Piezoelectric Properties ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Thermosensitives hydrogels based on poly (ethylene glycol): I. Synthesis, characterization and release

2007 ◽  
Vol 32 (5) ◽  
pp. 431-446 ◽  
Author(s):  
Tahar Bartil ◽  
Mahmoud Bounekhel ◽  
Cedric Calberg ◽  
Robert Jerome
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene
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