Characterization of Manganese(II) Binding Site Mutants of Manganese Peroxidase†

Biochemistry ◽  
1996 ◽  
Vol 35 (27) ◽  
pp. 8986-8994 ◽  
Author(s):  
Katsuyuki Kishi ◽  
Margo Kusters-van Someren ◽  
Mary B. Mayfield ◽  
Jie Sun ◽  
Thomas M. Loehr ◽  
...  
Keyword(s):  
Binding Site ◽  
Manganese Peroxidase

The Manganese Binding Site of Manganese Peroxidase: Characterization of an Asp179Asn Site-Directed Mutant Protein

Biochemistry ◽  
1995 ◽  
Vol 34 (33) ◽  
pp. 10620-10627 ◽  
Author(s):  
Margo Kusters-van Someren ◽  
Katsuyuki Kishi ◽  
Taina Lundell ◽  
Michael H. Gold
Keyword(s):  
Binding Site ◽  
Manganese Peroxidase ◽  
Mutant Protein ◽  
Manganese Binding Site

scholarly journals Construction and characterization of a manganese-binding site in cytochrome c peroxidase: towards a novel manganese peroxidase

1997 ◽  
Vol 4 (3) ◽  
pp. 215-221 ◽  
Author(s):  
Bryan K.S. Yeung ◽  
Xiaotang Wang ◽  
Jeffrey A. Sigman ◽  
Peter A. Petillo ◽  
Yi Lu
Keyword(s):  
Cytochrome C ◽  
Binding Site ◽  
Manganese Peroxidase ◽  
Cytochrome C Peroxidase ◽  
Manganese Binding Site

Characterization of the Fe(III) Binding Site in Sepia Eumelanin

2004 ◽  
Author(s):  
Alexander Samokhvalov ◽  
Yan Liu ◽  
John Simon
Keyword(s):  
Binding Site

Characterization of Nucleotide Binding ­Site-Encoding Genes in Sweetpotato, Ipomoea batatas(L.) Lam., and Their Response to Biotic and Abiotic Stresses

2021 ◽  
pp. 1-15
Author(s):  
Zengzhi Si ◽  
Yake Qiao ◽  
Kai Zhang ◽  
Zhixin Ji ◽  
Jinling Han
Keyword(s):  
Binding Site ◽  
Abiotic Stresses ◽  
Ipomoea Batatas ◽  
Expression Profiles ◽  
Nucleotide Binding ◽  
Regulatory Elements ◽  
Nucleotide Binding Site ◽  
Biotic And Abiotic Stresses ◽  
Encoding Genes

Sweetpotato, <i>Ipomoea batatas</i> (L.) Lam., is an important and widely grown crop, yet its production is affected severely by biotic and abiotic stresses. The nucleotide binding site (NBS)-encoding genes have been shown to improve stress tolerance in several plant species. However, the characterization of NBS-encoding genes in sweetpotato is not well-documented to date. In this study, a comprehensive analysis of NBS-encoding genes has been conducted on this species by using bioinformatics and molecular biology methods. A total of 315 NBS-encoding genes were identified, and 260 of them contained all essential conserved domains while 55 genes were truncated. Based on domain architectures, the 260 NBS-encoding genes were grouped into 6 distinct categories. Phylogenetic analysis grouped these genes into 3 classes: TIR, CC (I), and CC (II). Chromosome location analysis revealed that the distribution of NBS-encoding genes in chromosomes was uneven, with a number ranging from 1 to 34. Multiple stress-related regulatory elements were detected in the promoters, and the NBS-encoding genes’ expression profiles under biotic and abiotic stresses were obtained. According to the bioinformatics analysis, 9 genes were selected for RT-qPCR analysis. The results revealed that <i>IbNBS75</i>, <i>IbNBS219</i>, and <i>IbNBS256</i> respond to stem nematode infection; <i>Ib­NBS240</i>, <i>IbNBS90</i>, and <i>IbNBS80</i> respond to cold stress, while <i>IbNBS208</i>, <i>IbNBS71</i>, and <i>IbNBS159</i> respond to 30% PEG treatment. We hope these results will provide new insights into the evolution of NBS-encoding genes in the sweetpotato genome and contribute to the molecular breeding of sweetpotato in the future.

scholarly journals Characterization of the Calmodulin-binding Site of Nonerythroid α-Spectrin

1989 ◽  
Vol 264 (10) ◽  
pp. 5826-5830 ◽  
Author(s):  
T L Leto ◽  
S Pleasic ◽  
B G Forget ◽  
E J Benz ◽  
V T Marchesi
Keyword(s):  
Binding Site ◽  
Calmodulin Binding
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