Evidence for an Essential Lysyl Residue in Phospholipase D fromStreptomycessp. by Modification with Diethyl Pyrocarbonate and Pyridoxal 5-Phosphate

Biochemistry ◽  
1996 ◽  
Vol 35 (30) ◽  
pp. 9631-9636 ◽  
Author(s):  
Francesco Secundo ◽  
Giacomo Carrea ◽  
Paola D'Arrigo ◽  
Stefano Servi
Keyword(s):  
Phospholipase D ◽  
Diethyl Pyrocarbonate ◽  
Lysyl Residue

Using of Phospholipase D of Corynebacterium Pseudotuberculosis Ovis as Immunostimulant with Some Poultry Vaccines

2015 ◽  
Vol 43 (1) ◽  
pp. 174-181
Author(s):  
Marwah M ◽  
Hanan Mahmoud ◽  
Mona El-Shehedi ◽  
Namaa Mohamed
Keyword(s):  
Phospholipase D ◽  
Corynebacterium Pseudotuberculosis

Role of Phospholipase D Inhibitor in Regulating Expression of Senescencerelated Phospholipase D gene in Postharvest Longan Fruit

2019 ◽  
Vol 14 (7) ◽  
pp. 649-657 ◽  
Author(s):  
Li Li ◽  
Jiemin Li ◽  
Jian Sun ◽  
Ping Yi ◽  
Changbao Li ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Ascorbic Acid Content ◽  
Titratable Acidity ◽  
Significant Inhibition ◽  
Solid Content ◽  
Total Soluble Solid ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Soluble Solid ◽  
Pericarp Browning

Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit. Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature. Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit. Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage. Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.

scholarly journals The inactivation of saccharopine dehydrogenase (L-lysine-forming) by diethyl pyrocarbonate.

1980 ◽  
Vol 255 (3) ◽  
pp. 937-942
Author(s):  
M. Fujioka ◽  
Y. Takata ◽  
H. Ogawa ◽  
M. Okamoto
Keyword(s):  
Diethyl Pyrocarbonate ◽  
Saccharopine Dehydrogenase
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