Kinetic Basis for the Substrate Specificity during Hydrolysis of Phospholipids by Secreted Phospholipase A2†

Biochemistry ◽  
1996 ◽  
Vol 35 (29) ◽  
pp. 9375-9384 ◽  
Author(s):  
Joseph Rogers ◽  
Bao-Zhu Yu ◽  
Spyros V. Serves ◽  
Gerasimos M. Tsivgoulis ◽  
Demetrios N. Sotiropoulos ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Phospholipase A2 ◽  
Secreted Phospholipase A2 ◽  
Hydrolysis Of

scholarly journals Group V Secreted Phospholipase A2 Is Upregulated by IL-4 in Human Macrophages and Mediates Phagocytosis via Hydrolysis of Ethanolamine Phospholipids

2015 ◽  
Vol 194 (7) ◽  
pp. 3327-3339 ◽  
Author(s):  
Julio M. Rubio ◽  
Juan P. Rodríguez ◽  
Luis Gil-de-Gómez ◽  
Carlos Guijas ◽  
María A. Balboa ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Group V ◽  
Human Macrophages ◽  
Secreted Phospholipase A2 ◽  
Hydrolysis Of

Effects of the propeptide of group X secreted phospholipase A2 on substrate specificity and interfacial activity on phospholipid monolayers

Biochimie ◽  
2013 ◽  
Vol 95 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Vanessa Point ◽  
Anaïs Bénarouche ◽  
Ikram Jemel ◽  
Goetz Parsiegla ◽  
Gérard Lambeau ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Phospholipase A2 ◽  
Interfacial Activity ◽  
Phospholipid Monolayers ◽  
Secreted Phospholipase A2

scholarly journals Enhanced hydrolysis of phosphatidylcholine by human group II non-pancreatic secreted phospholipase A2 as a result of interfacial activation by specific anions. Potential role of cholesterol sulphate

1995 ◽  
Vol 308 (2) ◽  
pp. 507-512 ◽  
Author(s):  
A R Kinkaid ◽  
D C Wilton
Keyword(s):  
Phospholipase A2 ◽  
Anionic Phospholipid ◽  
Interfacial Activation ◽  
Secreted Phospholipase A2 ◽  
Cell Plasma ◽  
Membrane Hydrolysis ◽  
Membrane Bound ◽  
Group Ii ◽  
Hydrolysis Of

The extracellular concentration of the Group II human non-pancreatic secreted phospholipase A2 (hnpsPLA2) is elevated in a variety of inflammatory disorders. This enzyme is remarkable because it demonstrates almost zero activity with egg phosphatidylcholine (PC) or synthetic dioleoyl-phosphatidylcholine (DOPC) as substrate, but expresses high activity with the anionic phospholipid dioleoyl-phosphatidylglycerol (DOPG), a feature shared with the Group II enzyme from rat liver. The presence of certain membrane-bound anions can enhance hydrolysis of PC by the mammalian secreted PLA2S. In this study the ability of various non-polar anions to stimulate DOPC hydrolysis by secreted PLA2S has been investigated. The naturally occurring membrane anion, cholesterol sulphate, was particularly effective in stimulating the hydrolysis of both DOPC and also 1-stearoyl-2-arachidonyl phosphatidylcholine by hnpsPLA2. Activation of DOPC hydrolysis was also achieved with dioleoyl-phosphatidylserine (DOPS); however, DOPS was less effective than cholesterol sulphate. In contrast, the dianion dioleoyl-phosphatidic acid, a known activator of pig pancreatic PLA2, failed to activate the human enzyme. It remains to be established whether cell plasma-membrane hydrolysis by extracellular hnpsPLA2 can be activated in vivo by the presence of suitable membrane anions such as cholesterol sulphate and thus promote an inflammatory response within the cell.

scholarly journals Role of human group IIA secreted phospholipase A2 in malaria pathophysiology: Insights from a transgenic mouse model

Biochimie ◽  
2021 ◽  
Author(s):  
Mélanie Dacheux ◽  
Soraya Chaouch ◽  
Alonso Joy ◽  
Amandine Labat ◽  
Christine Payré ◽  
...  
Keyword(s):  
Mouse Model ◽  
Phospholipase A2 ◽  
Transgenic Mouse ◽  
Transgenic Mouse Model ◽  
Human Group ◽  
Secreted Phospholipase A2

scholarly journals Group X Secreted Phospholipase A2 Limits the Development of Atherosclerosis in LDL Receptor–Null Mice

2013 ◽  
Vol 33 (3) ◽  
pp. 466-473 ◽  
Author(s):  
Hafid Ait-Oufella ◽  
Olivier Herbin ◽  
Charlotte Lahoute ◽  
Christelle Coatrieux ◽  
Xavier Loyer ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Ldl Receptor ◽  
Secreted Phospholipase A2

scholarly journals Platelets release mitochondria serving as substrate for bactericidal group IIA-secreted phospholipase A2 to promote inflammation

Blood ◽  
2014 ◽  
Vol 124 (14) ◽  
pp. 2173-2183 ◽  
Author(s):  
Luc H. Boudreau ◽  
Anne-Claire Duchez ◽  
Nathalie Cloutier ◽  
Denis Soulet ◽  
Nicolas Martin ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Rickettsia Prowazekii ◽  
Platelet Storage ◽  
Secreted Phospholipase A2 ◽  
Molecular Pattern ◽  
Key Points

Key Points When activated and in platelet storage bags, platelets release respiratory-competent mitochondria, a recognized damage-associated molecular pattern. Mitochondria, descendant of Rickettsia prowazekii, serve as substrate for bactericidal sPLA2-IIA to promote inflammation.

scholarly journals Erratum to: “Self-assembling catalytic systems based on new amphiphile containing purine fragment, exhibiting substrate specificity in hydrolysis of phosphorus acids esters”

2017 ◽  
Vol 87 (7) ◽  
pp. 1649-1649
Author(s):  
D. A. Samarkina ◽  
D. R. Gabdrakhmanov ◽  
V. E. Semenov ◽  
F. G. Valeeva ◽  
L. M. Gubaidullina ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Catalytic Systems ◽  
Self Assembling ◽  
Hydrolysis Of
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