Rabbit Muscle Creatine Kinase:  Consequences of the Mutagenesis of Conserved Histidine Residues†

Biochemistry ◽  
1996 ◽  
Vol 35 (24) ◽  
pp. 7895-7902 ◽  
Author(s):  
Lorenzo H. Chen ◽  
Charles L. Borders, ◽  
John R. Vásquez ◽  
George L. Kenyon
Keyword(s):  
Creatine Kinase ◽  
Rabbit Muscle ◽  
Muscle Creatine Kinase ◽  
Histidine Residues ◽  
Muscle Creatine

Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase

2006 ◽  
Vol 84 (2) ◽  
pp. 142-147
Author(s):  
Feng Shi ◽  
Tong-Jin Zhao ◽  
Hua-Wei He ◽  
Jie Li ◽  
Xian-Gang Zeng ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Energy Homeostasis ◽  
Inactivation Kinetics ◽  
Rabbit Muscle ◽  
Muscle Creatine Kinase ◽  
Reversible Inactivation ◽  
Inhibition Effect ◽  
Sodium Barbital ◽  
Muscle Creatine ◽  
Detailed Kinetics

As a depressant of the central nervous system, the clinical effect of sodium barbital has been extensively studied. Here we report on sodium barbital as an inhibitor of rabbit-muscle creatine kinase (CK), which plays a significant role in energy homeostasis in the muscles. Although sodium barbital gradually inhibits the activity of CK with increased concentration, the inhibition effect can be completely reversed by dilution, indicating that the inactivation process is reversible. Detailed kinetics analysis, according to a previously presented theory, indicates that sodium barbital functions as a non complexing inhibitor, and its inhibition effect on CK is a slow reversible inactivation. In this study, a kinetic model of the substrate reaction is presented, and the microscopic rate constants for the reaction of sodium barbital with the free enzyme and the enzyme–substrate complexes are determined. Kinetic analysis reveals that sodium barbital might compete with both creatine and ATP, but mainly with creatine, to inhibit the activity of CK. The results suggest that CK might be a target for sodium barbital in vivo.Key words: creatine kinase; inactivation; kinetics; sodium barbital.

scholarly journals Chaperone-Like Effect of the Linker on the Isolated C-Terminal Domain of Rabbit Muscle Creatine Kinase

2012 ◽  
Vol 103 (3) ◽  
pp. 558-566 ◽  
Author(s):  
Zhe Chen ◽  
Xiang-Jun Chen ◽  
Mengdie Xia ◽  
Hua-Wei He ◽  
Sha Wang ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Rabbit Muscle ◽  
Muscle Creatine Kinase ◽  
Terminal Domain ◽  
Muscle Creatine
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