Interactions Involving the Human RNA Polymerase II Transcription/Nucleotide Excision Repair Complex TFIIH, the Nucleotide Excision Repair Protein XPG, and Cockayne Syndrome Group B (CSB) Protein†

Biochemistry ◽  
1996 ◽  
Vol 35 (7) ◽  
pp. 2157-2167 ◽  
Author(s):  
Narayan Iyer ◽  
Michael S. Reagan ◽  
Kou-Juey Wu ◽  
Bertram Canagarajah ◽  
Errol C. Friedberg
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Cockayne Syndrome ◽  
Repair Protein ◽  
Nucleotide Excision ◽  
Group B ◽  
Rna Polymerase Ii Transcription ◽  
Nucleotide Excision Repair Protein

scholarly journals The yeast TFB1 and SSL1 genes, which encode subunits of transcription factor IIH, are required for nucleotide excision repair and RNA polymerase II transcription.

1995 ◽  
Vol 15 (4) ◽  
pp. 2288-2293 ◽  
Author(s):  
Z Wang ◽  
S Buratowski ◽  
J Q Svejstrup ◽  
W J Feaver ◽  
X Wu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Yeast Saccharomyces Cerevisiae ◽  
Nucleotide Excision ◽  
Rna Polymerase Ii Transcription

The essential TFB1 and SSL1 genes of the yeast Saccharomyces cerevisiae encode two subunits of the RNA polymerase II transcription factor TFIIH (factor b). Here we show that extracts of temperature-sensitive mutants carrying mutations in both genes (tfb1-101 and ssl1-1) are defective in nucleotide excision repair (NER) and RNA polymerase II transcription but are proficient for base excision repair. RNA polymerase II-dependent transcription at the CYC1 promoter was normal at permissive temperatures but defective in extracts preincubated at a restrictive temperature. In contrast, defective NER was observed at temperatures that are permissive for growth. Additionally, both mutants manifested increased sensitivity to UV radiation at permissive temperatures. The extent of this sensitivity was not increased in a tfb1-101 strain and was only slightly increased in a ssl1-1 strain at temperatures that are semipermissive for growth. Purified factor TFIIH complemented defective NER in both tfb1-101 and ssl1-1 mutant extracts. These results define TFB1 and SSL1 as bona fide NER genes and indicate that, as is the case with the yeast Rad3 and Ss12 (Rad25) proteins, Tfb1 and Ssl1 are required for both RNA polymerase II basal transcription and NER. Our results also suggest that the repair and transcription functions of Tfb1 and Ssl1 are separable.

scholarly journals Yeast RNA Polymerase II Transcription In Vitro Is Inhibited in the Presence of Nucleotide Excision Repair: Complementation of Inhibition by Holo-TFIIH and Requirement for RAD26

1998 ◽  
Vol 18 (5) ◽  
pp. 2668-2676 ◽  
Author(s):  
Zhaoyang You ◽  
William J. Feaver ◽  
Errol C. Friedberg
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Cell Extracts ◽  
Nucleotide Excision ◽  
Rnap Ii ◽  
Group B ◽  
Damaged Dna

ABSTRACT The Saccharomyces cerevisiae transcription factor IIH (TFIIH) is essential both for transcription by RNA polymerase II (RNAP II) and for nucleotide excision repair (NER) of damaged DNA. We have established cell extracts which support RNAP II transcription from the yeast CYC1 promoter or NER of transcriptionally silent damaged DNA on independent plasmid templates and substrates. When plasmid templates and substrates for both processes are simultaneously incubated with these extracts, transcription is significantly inhibited. This inhibition is strictly dependent on active NER and can be complemented with purified holo-TFIIH. These results suggest that in the presence of active NER, TFIIH is preferentially mobilized from the basal transcription machinery for use in NER. Inhibition of transcription in the presence of active NER requires theRAD26 gene, the yeast homolog of the human Cockayne syndrome group B gene (CSB).

scholarly journals Structure of UvrA nucleotide excision repair protein in complex with modified DNA

2011 ◽  
Vol 18 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Marcin Jaciuk ◽  
Elżbieta Nowak ◽  
Krzysztof Skowronek ◽  
Anna Tańska ◽  
Marcin Nowotny
Keyword(s):  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Repair Protein ◽  
Modified Dna ◽  
Nucleotide Excision ◽  
Nucleotide Excision Repair Protein

The nucleotide excision repair protein UvrB, a helicase-like enzyme with a catch

2000 ◽  
Vol 460 (3-4) ◽  
pp. 277-300 ◽  
Author(s):  
Karsten Theis ◽  
Milan Skorvaga ◽  
Mischa Machius ◽  
Noriko Nakagawa ◽  
Bennett Van Houten ◽  
...  
Keyword(s):  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Repair Protein ◽  
Nucleotide Excision ◽  
Nucleotide Excision Repair Protein
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