Microcirculating System for Simultaneous Determination of Raman and Absorption Spectra of Enzymatic Reaction Intermediates and Its Application to the Reaction of CytochromecOxidase with Hydrogen Peroxide

Biochemistry ◽  
1996 ◽  
Vol 35 (1) ◽  
pp. 76-82 ◽  
Author(s):  
Denis A. Proshlyakov ◽  
Takashi Ogura ◽  
Kyoko Shinzawa-Itoh ◽  
Shinya Yoshikawa ◽  
Teizo Kitagawa
Keyword(s):  
Hydrogen Peroxide ◽  
Simultaneous Determination ◽  
Absorption Spectra ◽  
Enzymatic Reaction ◽  
Reaction Intermediates

Enzymatic colorimetry of lecithin and sphingomyelin in aqueous solution.

1983 ◽  
Vol 29 (8) ◽  
pp. 1513-1517 ◽  
Author(s):  
M W McGowan ◽  
J D Artiss ◽  
B Zak
Keyword(s):  
Aqueous Solution ◽  
Hydrogen Peroxide ◽  
Amniotic Fluid ◽  
Enzymatic Reaction ◽  
Detergent Solution ◽  
Enzymatic Determination ◽  
Red Color ◽  
Hydrolysis Of ◽  
Zwitterionic Detergent

Abstract A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, which is then oxidized to betaine, thus generating hydrogen peroxide. The hydrogen peroxide is subsequently utilized in the enzymatic coupling of 4-aminoantipyrine and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate, an intensely red color being formed. The presence of a non-reacting phospholipid enhances the hydrolysis of the reacting phospholipid. Thus we added lecithin to the sphingomyelin standards and sphingomyelin to the lecithin standards. This precise procedure may be applicable to determination of lecithin and sphingomyelin in amniotic fluid.

scholarly journals Development of multivariate calibration method for simultaneous determination of nickel, lead and zinc in tap water

2015 ◽  
Vol 18 (2) ◽  
pp. 145-151
Author(s):  
Chau Minh Huynh ◽  
Thu Du Ly ◽  
Thach Thai Pham ◽  
Tran Thi Bao Pham ◽  
Minh Khanh Duong ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Absorption Spectra ◽  
Multivariate Calibration ◽  
Tap Water ◽  
Calibration Method ◽  
Calibration Model ◽  
Determination Of Nickel ◽  
Lead And Zinc ◽  
Nickel Lead

Conventional spectrophotometric methods for simultaneous determination of nickel, lead and zinc in forms of complexes with a reagent is not feasible due to the overlap of their absorption spectra. A multivariate calibration method was used to overcome this problem. In this study, the calibration model was constructed based on absorption spectra of 30 mixture standards in the range from 490 to 600 nm. Factors influencing experimental results such as amount of reagents, pH, and color development time were optimized. The standard calibration ranges for determination of nickel, lead and zinc were found at 0.5-5 ppm. The method was applied for determination of these ions in tap water samples at ppm level, with recoveries (and RSD) of nickel, lead and zinc were 103.3 % (3.0 %), 74.9 % (11.5 %) and 104.6 % (4.6 %), respectively.

Simultaneous determination of ascorbic acid, dopamine, uric acid and hydrogen peroxide based on co-immobilization of PEDOT and FAD using multi-walled carbon nanotubes

2014 ◽  
Vol 6 (20) ◽  
pp. 8321-8327 ◽  
Author(s):  
Kuo Chiang Lin ◽  
Jia Yan Huang ◽  
Shen Ming Chen
Keyword(s):  
Carbon Nanotubes ◽  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Uric Acid ◽  
Simultaneous Determination ◽  
High Conductivity ◽  
Hybrid Films ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Illustration of electro-codeposition of PEDOT and FAD hybrid films using high conductivity and steric MWCNTs as a template.

Simultaneous determination of peracetic acid and acetic acid by titration with NaOH

2014 ◽  
Vol 6 (7) ◽  
pp. 2406-2409 ◽  
Author(s):  
Frank Sode
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Simultaneous Determination ◽  
Peracetic Acid ◽  
Easy Method

This paper describes a fast and easy method to analyze peracetic acid in mixtures with both acetic acid and hydrogen peroxide.

scholarly journals Application Enzymatic Method for Analysis Vaginal Tabletas “Fluomizin”

2021 ◽  
Vol 62 (3) ◽  
pp. 260-269
Author(s):  
Koval'ska Olena Vasilivna ◽  
Blazheyevskіy Mykola
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Reaction ◽  
Chloride Content ◽  
Solution Concentration ◽  
Photometric Method ◽  
Maximal Velocity ◽  
Buffer Solution ◽  
Dequalinium Chloride ◽  
Hydrolysis Of

Introduction: Development of a new enzymatic kinetic-photometric method for analysis of Dequalinium chloride, based on the use of enzymatic hydrolysis of acetylcholine, for determine the amount of Dequalinium chloride in the vaginal tablets “Fluomizin” No 6. Method: The amount of Dequalinium chloride (DCh) was determined by the degree of inhibition of the enzymatic reaction, which was evaluated by the residual unreacted substrate - acetylcholine. Determination of the residual amount of acetylcholine in the reaction mixture was performed by a kinetic-photometric method using an indicator oxidation reaction of p-phenetidine with peracetic acid, which is formed during the auxiliary reaction of perhydrolysis with addition of excess hydrogen peroxide in the reaction mixture over a period of time. Results: Optimal conditions were determined: pH 8.35 was selected, influence of nature of buffer solution, concentration of acetylcholine (0.05 mg / ml), cholinesterase (0.4 mg / ml), hydrogen peroxide (10,0%) and p-phenethedine (1 %), the incubation time (10 min). Conclusions: The procedure for determination of Dequalinium chloride content in the vaginal tablets “Fluomizin” No 6 was developed. The kinetic parameters Km (Michaelis–Menten constant) and Vmax (maximal velocity) were determined.

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