scholarly journals The Membrane Topography of the Diphtheria Toxin T Domain Linked to the A Chain Reveals a Transient Transmembrane Hairpin and Potential Translocation Mechanisms

Biochemistry ◽  
2009 ◽  
Vol 48 (43) ◽  
pp. 10446-10456 ◽  
Author(s):  
Jie Wang ◽  
Erwin London
Keyword(s):  
Diphtheria Toxin ◽  
Membrane Topography ◽  
T Domain ◽  
A Chain

scholarly journals Mapping the Membrane Topography of Segments TH6-TH7 and TH8-TH9 in the Diphtheria Toxin T-Domain Channel

2013 ◽  
Vol 104 (2) ◽  
pp. 240a
Author(s):  
Paul Kienker ◽  
Zhengyan Wu ◽  
Alan Finkelstein
Keyword(s):  
Diphtheria Toxin ◽  
Membrane Topography ◽  
T Domain

Membrane Topography of the T Domain of Diphtheria Toxin Probed with Single Tryptophan Mutants†

Biochemistry ◽  
1998 ◽  
Vol 37 (51) ◽  
pp. 17915-17922 ◽  
Author(s):  
Susan E. Malenbaum ◽  
R. John Collier ◽  
Erwin London
Keyword(s):  
Diphtheria Toxin ◽  
Membrane Topography ◽  
T Domain

scholarly journals pH-Triggered Membrane Insertion Pathway of the Diphtheria Toxin T-Domain: 1. Insertion/Refolding Intermediate

2009 ◽  
Vol 96 (3) ◽  
pp. 433a
Author(s):  
Alexander Kyrychenko ◽  
Mykola V. Rodnin ◽  
Yevgen O. Posokhov ◽  
Alexey S. Ladokhin
Keyword(s):  
Diphtheria Toxin ◽  
Membrane Insertion ◽  
T Domain

Organization of Diphtheria Toxin T Domain in Bilayers: A Site-Directed Spin Labeling Study

Science ◽  
1996 ◽  
Vol 273 (5276) ◽  
pp. 810-812 ◽  
Author(s):  
K. J. Oh ◽  
H. Zhan ◽  
C. Cui ◽  
K. Hideg ◽  
R. J. Collier ◽  
...  
Keyword(s):  
Diphtheria Toxin ◽  
Spin Labeling ◽  
Site Directed Spin Labeling ◽  
T Domain ◽  
A Site

scholarly journals Cloning, sequence determination, and expression in transfected cells of the coding sequence for the tox 176 attenuated diphtheria toxin A chain.

1987 ◽  
Vol 7 (4) ◽  
pp. 1576-1579 ◽  
Author(s):  
F Maxwell ◽  
I H Maxwell ◽  
L M Glode
Keyword(s):  
Diphtheria Toxin ◽  
Predicted Amino Acid Sequence ◽  
Specific Cell ◽  
Wild Type ◽  
Transient Expression Assay ◽  
Toxin A ◽  
Coding Sequence ◽  
Chain Coding ◽  
Diphtheria Toxin A ◽  
A Chain

DNA including the coding sequence for the A chain of the mutant diphtheria toxin tox 176 was cloned. The cloned mature A-chain coding sequence showed a G-to-A transition at nucleotide 383 as the only difference from the wild-type sequence. This resulted in replacement of the glycine at position 128 by aspartic acid in the predicted amino acid sequence. A eucaryotic cell expression plasmid, pTH1-176, was constructed in which the tox 176 A-chain coding sequence was attached to a truncated metallothionein promoter. The toxicity of this construct, compared with that of the corresponding wild-type diphtheria toxin A-chain plasmid, pTH1, was assessed after transfection into the human 293 cell line by an indirect transient expression assay (I. H. Maxwell, F. Maxwell, and L. M. Glode, Cancer Res. 46:4660-4664, 1986). For the same effect, 15- to 30-fold more pTH1-176 than pTH1 was required, a result consistent with previous in vitro estimates of the diminished activity of the tox 176 A chain. Controlled expression of the cloned tox 176 A-chain coding sequence may provide a means of eliminating specific cell populations in an organism, for which purpose the wild-type diphtheria toxin A chain might prove too toxic.

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