scholarly journals Correction to A Molecular Mimic of Phosphorylated Prolactin (S179D PRL) Secreted by Eukaryotic Cells Has a Conformation with an Increased Positive Surface Charge Compared to That of Unmodified Prolactin

Biochemistry ◽  
2009 ◽  
Vol 48 (36) ◽  
pp. 8764-8764
Author(s):  
Eric K. M. Ueda ◽  
Carlos R. J. Soares ◽  
Paolo Bartolini ◽  
Ariel DeGuzman ◽  
Mary Y. Lorenson ◽  
...  
Keyword(s):  
Surface Charge ◽  
Eukaryotic Cells ◽  
Positive Surface Charge ◽  
Phosphorylated Prolactin

A Molecular Mimic of Phosphorylated Prolactin (S179D PRL) Secreted by Eukaryotic Cells Has a Conformation with an Increased Positive Surface Charge Compared to That of Unmodified Prolactin

Biochemistry ◽  
2009 ◽  
Vol 48 (29) ◽  
pp. 6887-6897 ◽  
Author(s):  
Eric K. M. Ueda ◽  
Carlos R. J. Soares ◽  
Paolo Bartolini ◽  
Ariel DeGuzman ◽  
Mary Y. Lorenson ◽  
...  
Keyword(s):  
Surface Charge ◽  
Eukaryotic Cells ◽  
Positive Surface Charge ◽  
Phosphorylated Prolactin

scholarly journals Characterization of Liposomes for Cancer Cell Transfection

2007 ◽  
Vol 1 (1) ◽  
pp. 60-63
Author(s):  
Svetlana A Tatarkova ◽  
Satvinder Khaira
Keyword(s):  
Surface Charge ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Surface Potential ◽  
Cell Transfection ◽  
Similar Extent ◽  
Positive Surface Charge ◽  
Negative Surface ◽  
Subsequent Addition

We have characterized a broad range of liposome formulations with varying DcChol:DOPE ratio. Subsequent addition of DcChol to liposomes increases its positive surface charge. However, loading the nuclear acids did not neutralize the overall negative surface potential to a similar extent. The liposomes were tested by transfection of DNA in living cancer cells.

Electrostatic-driven solid phase microextraction coupled with surface enhanced Raman spectroscopy for rapid analysis of pentachlorophenol

2017 ◽  
Vol 9 (3) ◽  
pp. 459-464 ◽  
Author(s):  
Weiwei Bian ◽  
Sha Zhu ◽  
Mingying Qi ◽  
Lanlan Xiao ◽  
Zhen Liu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Charge ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Surface Enhanced Raman Spectroscopy ◽  
Rapid Analysis ◽  
Positive Surface Charge ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Ag Substrate

Rapid analysis of pentachlorophenol by electrostatic-driven SPME–SERS on a nanoporous Ag substrate with positive surface charge.

Carbon dots with positive surface charge from tartaric acid and m-aminophenol for selective killing of Gram-positive bacteria

2021 ◽  
Vol 9 (1) ◽  
pp. 125-130
Author(s):  
Huibo Wang ◽  
Fang Lu ◽  
Chongqing Ma ◽  
Yurong Ma ◽  
Mengling Zhang ◽  
...  
Keyword(s):  
Surface Charge ◽  
Tartaric Acid ◽  
Carbon Dots ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Positive Surface Charge

Carbon dots with positive surface charge from tartaric acid and m-aminophenol for selective killing of Gram-positive bacteria.

scholarly journals The optimal pH of AID is skewed from that of its catalytic pocket by DNA-binding residues and surface charge

2021 ◽  
Author(s):  
Atefeh Ghorbani ◽  
Justin John King ◽  
Mani Larijani
Keyword(s):  
Catalytic Activity ◽  
Surface Charge ◽  
Cytidine Deaminase ◽  
Inverse Correlation ◽  
Acidic Ph ◽  
Structural Mechanism ◽  
Positive Surface Charge ◽  
Equivalent Residue ◽  
Binding Residues ◽  
Optimal Ph

Activation-induced cytidine deaminase (AID) is a member of the apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) family of cytidine deaminases. AID mutates immunoglobulin loci to initiate secondary antibody diversification. The APOBEC3 (A3) sub-branch mutates viral pathogens in the cytosol and acidic endosomal compartments. Accordingly, AID functions optimally near neutral pH, while most A3s are acid-adapted (optimal pH 5.5-6.5). To gain a structural understanding for this pH disparity, we constructed high-resolution maps of AID catalytic activity vs pH. We found AID’s optimal pH was 7.3 but it retained most (>70%) of the activity at pH 8. Probing of ssDNA-binding residues near the catalytic pocket, key for bending ssDNA into the pocket (e.g R25) yielded mutants with altered pH preference, corroborating previous findings that the equivalent residue in APOBEC3G (H216) underlies its acidic pH preference. AID from bony fish exhibited more basic optimal pH (pH 7.5-8.1) and several R25-equivalent mutants altered pH preference. Comparison of pH optima across the AID/APOBEC3 family revealed an inverse correlation between positive surface charge and overall catalysis.  The paralogue with the most robust catalytic activity (APOBEC3A) has the lowest surface charge, most acidic pH preference, while the paralogue with the most lethargic catalytic rate (AID) has the most positive surface charge and highest optimal pH. We suggest one possible mechanism is through surface charge dictating an overall optimal pH that is different from the optimal pH of the catalytic pocket microenvironment. These findings illuminate an additional structural mechanism that regulates AID/APOBEC3 mutagenesis.

Eudragit RL100 Based Moxifloxacin Hydrochloride and Ketorolac Tromethamine Combination Nanoparticulate System for Ocular Drug Delivery

2020 ◽  
Vol 8 (2) ◽  
pp. 133-147
Author(s):  
Vedanti Salvi ◽  
Pravin Pawar
Keyword(s):  
Drug Delivery ◽  
Surface Charge ◽  
Ex Vivo ◽  
Fold Increase ◽  
Ocular Drug Delivery ◽  
Water Soluble ◽  
Ocular Infection ◽  
Ketorolac Tromethamine ◽  
Bacterial Conjunctivitis ◽  
Positive Surface Charge

Background: Bacterial conjunctivitis is a serious ocular infection if left untreated. It is caused by several species of bacteria like Pseudomonas, Staphylococcus and Mycobacterium. Objective: The present investigation explores the development and characterization of moxifloxacin hydrochloride and ketorolac tromethamine combination loaded Eudragit RL 100 nanosuspension for ocular drug delivery in order to overcome the problems associated with conventional dosage forms. Methods: The nanosuspension prepared by nanoprecipitation technique showed successful entrapment of both water-soluble drugs in the polymer matrix indicated by their % entrapment efficiencies. Results: Formulations showed a mean particle size <200 nm with narrow size distribution and positive surface charge due to the presence of quaternary ammonium groups of Eudragit RL100. FTIR study revealed compatibility among the components, while a reduction in the crystallinity of formulation was observed in the PXRD study. The release of both the drugs was found to be sustained in nanosuspension as compared to commercial eyedrops. Ex vivo studies showed increased transcorneal permeation of drugs from nanosuspension, where approximately 2.5-fold and 2-fold increase in the permeation was observed for moxifloxacin hydrochloride and ketorolac tromethamine, respectively. The formulation was stable at 4°C and room temperature. Conclusion: Due to their sustained release, positive surface charge and higher transcorneal permeation, this will be a promising ocular drug delivery.

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