Signal Transducer and Activator of Transcription 6 (STAT6) Is a Novel Interactor of Annexin A2 in Prostate Cancer Cells

Biochemistry ◽  
2010 ◽  
Vol 49 (10) ◽  
pp. 2216-2226 ◽  
Author(s):  
Susobhan Das ◽  
Praveenkumar Shetty ◽  
Mallika Valapala ◽  
Subhamoy Dasgupta ◽  
Zygmunt Gryczynski ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Annexin A2 ◽  
Signal Transducer ◽  
Prostate Cancer Cells

scholarly journals Transcription Factor Signal Transducer and Activator of Transcription 5 Promotes Growth of Human Prostate Cancer Cells In vivo

2008 ◽  
Vol 14 (5) ◽  
pp. 1317-1324 ◽  
Author(s):  
Ayush Dagvadorj ◽  
Robert A. Kirken ◽  
Benjamin Leiby ◽  
James Karras ◽  
Marja T. Nevalainen
Keyword(s):  
Prostate Cancer ◽  
Transcription Factor ◽  
Cancer Cells ◽  
Human Prostate ◽  
Signal Transducer ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells

scholarly journals Cryptotanshinone Inhibits Constitutive Signal Transducer and Activator of Transcription 3 Function through Blocking the Dimerization in DU145 Prostate Cancer Cells

2008 ◽  
Vol 69 (1) ◽  
pp. 193-202 ◽  
Author(s):  
Dae-Seop Shin ◽  
Hye-Nan Kim ◽  
Ki Deok Shin ◽  
Young Ju Yoon ◽  
Seung-Jun Kim ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Signal Transducer ◽  
Prostate Cancer Cells ◽  
Transcription 3

scholarly journals Ginkgetin inhibits the growth of DU −145 prostate cancer cells through inhibition of signal transducer and activator of transcription 3 activity

2015 ◽  
Vol 106 (4) ◽  
pp. 413-420 ◽  
Author(s):  
Yoon Jung Jeon ◽  
Seung‐Nam Jung ◽  
Jieun Yun ◽  
Chang Woo Lee ◽  
Jiyeon Choi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Signal Transducer ◽  
Prostate Cancer Cells ◽  
Transcription 3

scholarly journals Acacetin Inhibits the Growth of STAT3-Activated DU145 Prostate Cancer Cells by Directly Binding to Signal Transducer and Activator of Transcription 3 (STAT3)

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6204
Author(s):  
Sun Yun ◽  
Yu-Jin Lee ◽  
Jiyeon Choi ◽  
Nam Doo Kim ◽  
Dong Cho Han ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Nuclear Translocation ◽  
Critical Role ◽  
Cancer Drug ◽  
Dependent Manner ◽  
Signal Transducer ◽  
Prostate Cancer Cells ◽  
Stat3 Phosphorylation ◽  
Transcription 3

Signal transducer and activator of transcription 3 (STAT3) plays a critical role in the formation and growth of human cancer. Therefore, STAT3 is a therapeutic target for cancer drug discovery. Acacetin, a flavone present in various plants, inhibits constitutive and inducible STAT3 activation in STAT3-activated DU145 prostate cancer cells. Acacetin inhibits STAT3 activity by directly binding to STAT3, which we confirmed by a pull-down assay with a biotinylated compound and two level-free methods, namely, a drug affinity responsive target stability (DARTS) experiment and a cellular thermal shift assay (CETSA). Acacetin inhibits STAT3 phosphorylation at the tyrosine 705 residue and nuclear translocation in DU145 cells, which leads to the downregulation of STAT3 target genes. Acacetin then induces apoptosis in a time-dependent manner. Interestingly, acacetin induces the production of reactive oxygen species (ROS) that are not involved in the acacetin-induced inhibition of STAT3 activation because the suppressed p-STAT3 level is not rescued by treatment with GSH or NAC, which are general ROS inhibitors. We also found that acacetin inhibits tumor growth in xenografted nude mice. These results suggest that acacetin, as a STAT3 inhibitor, could be a possible drug candidate for targeting STAT3 for the treatment of cancer in humans.

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