Analysis of the Interaction of BCL9 with β-Catenin and Development of Fluorescence Polarization and Surface Plasmon Resonance Binding Assays for this Interaction

Biochemistry ◽  
2009 ◽  
Vol 48 (40) ◽  
pp. 9534-9541 ◽  
Author(s):  
Steven A. Kawamoto ◽  
Andrea D. Thompson ◽  
Adriana Coleska ◽  
Zaneta Nikolovska-Coleska ◽  
Han Yi ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Fluorescence Polarization ◽  
Binding Assays

scholarly journals Insights into PARP Inhibitors’ Selectivity Using Fluorescence Polarization and Surface Plasmon Resonance Binding Assays

2014 ◽  
Vol 19 (8) ◽  
pp. 1212-1219 ◽  
Author(s):  
Gianluca Papeo ◽  
Nilla Avanzi ◽  
Serena Bettoni ◽  
Antonella Leone ◽  
Mauro Paolucci ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Fluorescence Polarization ◽  
High Throughput Screening ◽  
Catalytic Domain ◽  
Parp Inhibitors ◽  
Binding Assays ◽  
Double Knockout ◽  
Parp 1

PARP inhibitors are an exciting new class of antineoplastic drugs that have been proven to be efficacious as single agents in cancer settings with inherent DNA repair defects, as well as in combination with DNA-damaging chemotherapeutics. Currently, they are designed to target the catalytic domain of PARP-1, the most studied member of the family, with a key role in the DNA-damage repair process. Because PARP inhibitors are substrate (NAD+) competitors, there is a need for a deeper understanding of their cross-reactivity. This is particularly relevant for PARP-2, the PARP-1 closest homologue, for which an embryonic lethal phenotype has been observed in double knockout mice. In this study, we describe the development and validation of binding assays based on fluorescence polarization (FP) and surface plasmon resonance (SPR) techniques. PARP-1, PARP-2, PARP-3, and TNKS-1 FP displacement assays are set up by employing ad hoc synthesized probes. These assays are suitable for high-throughput screening (HTS) and selectivity profiling, thus allowing the identification of NAD+ binding site selective inhibitors. The PARP-1 and PARP-2 complementary SPR binding assays confirm displacement data and the in-depth inhibitor characterization. Moreover, these formats have the potential to be broadly applicable to other members of the PARP family.

Binding assays with artificial tethered membranes using surface plasmon resonance

Methods ◽  
2006 ◽  
Vol 39 (2) ◽  
pp. 134-146 ◽  
Author(s):  
Birgit Wiltschi ◽  
Wolfgang Knoll ◽  
Eva-Kathrin Sinner
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assays ◽  
Tethered Membranes

Characterization of labeled reagents in ligand-binding assays by a surface plasmon resonance biosensor

Bioanalysis ◽  
2017 ◽  
Vol 9 (2) ◽  
pp. 193-207 ◽  
Author(s):  
Jia Duo ◽  
JoAnne Bruno ◽  
Steven Piccoli ◽  
Binodh DeSilva ◽  
Yan J Zhang
Keyword(s):  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assays ◽  
Surface Plasmon Resonance Biosensor

Src homology-2 domain binding assays by scintillation proximity and surface plasmon resonance

2001 ◽  
Vol 14 (4) ◽  
pp. 254-260 ◽  
Author(s):  
Eliane Mandine ◽  
Dominique Gofflo ◽  
V�ronique Jean-Baptiste ◽  
Edoardo Sarubbi ◽  
Gaetan Touyer ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assays ◽  
Src Homology 2 ◽  
Src Homology ◽  
Src Homology 2 Domain

scholarly journals The immunomodulatory dCache chemoreceptor TlpA of Helicobacter pylori binds multiple attractant and antagonistic ligands via distinct sites

2021 ◽  
Author(s):  
Kevin S. Johnson ◽  
Bassam Elgamoudi ◽  
Freda E.-C. Jen ◽  
Christopher J. Day ◽  
Emily Goers Sweeney ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Molecular Docking ◽  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assays ◽  
Binding Pockets

The Helicobacter pylori chemoreceptor TlpA plays a role in dampening host inflammation during chronic stomach colonization. TlpA has a periplasmic dCache_1 domain, a structure that is capable of sensing many ligands; however, the only characterized TlpA signals are arginine, bicarbonate, and acid. To increase our understanding of TlpA's sensing profile, we screened for diverse TlpA ligands using ligand binding arrays. TlpA bound seven ligands with affinities in the low to middle micromolar ranges. Three of these ligands, arginine, fumarate, and cysteine, were TlpA-dependent chemoattractants, while the others elicited no response. Molecular docking experiments, site-directed point mutants, and competition surface plasmon resonance binding assays suggested that TlpA binds ligands via both the membrane-distal and -proximal dCache_1 binding pockets. Surprisingly, one of the non-active ligands, glucosamine, acted as a chemotaxis antagonist, preventing the chemotaxis response to chemoattractant ligands and acted to block binding of ligands irrespective of whether they bound the membrane-distal or -proximal dCache_1 subdomains. In total, these results suggest TlpA senses multiple attractant ligands as well as antagonist ones, an emerging theme in chemotaxis systems.

scholarly journals A Suite of Biochemical Assays for Screening RNA Methyltransferase BCDIN3D

2016 ◽  
Vol 22 (1) ◽  
pp. 32-39
Author(s):  
Levi L. Blazer ◽  
Fengling Li ◽  
Steven Kennedy ◽  
Yujun George Zheng ◽  
Cheryl H. Arrowsmith ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Small Molecule ◽  
Plasmon Resonance ◽  
Fluorescence Polarization ◽  
Small Molecule Inhibitors ◽  
Biochemical Assays ◽  
Differential Scanning Fluorimetry ◽  
Small Molecule Modulators ◽  
Displacement Assays

BCDIN3D is an RNA-methyltransferase that O-methylates the 5′ phosphate of RNA and regulates microRNA maturation. To discover small-molecule inhibitors of BCDIN3D, a suite of biochemical assays was developed. A radiometric methyltransferase assay and fluorescence polarization–based S-adenosylmethionine and RNA displacement assays are described. In addition, differential scanning fluorimetry and surface plasmon resonance were used to characterize binding. These assays provide a comprehensive package for the development of small-molecule modulators of BCDIN3D activity.

Comparison of sensitivity of the refractometric methods of frustrated total internal reflection and surface plasmon resonance

2020 ◽  
pp. 44-49
Author(s):  
I. N. Pavlov
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Total Internal Reflection ◽  
Resonance Method ◽  
Internal Reflection ◽  
Optical Methods ◽  
Thin Boundary Layer ◽  
Experimental Conditions ◽  
Frustrated Total Internal Reflection

Two optical methods, namely surface plasmon resonance imaging and frustrated total internal reflection, are described in the paper in terms of comparing their sensitivity to change of refractive index of a thin boundary layer of an investigated medium. It is shown that, despite the fact that the theoretically calculated sensitivity is higher for the frustrated total internal reflection method, and the fact that usually in practice the surface plasmon resonance method, on the contrary, is considered more sensitive, under the same experimental conditions both methods show a similar result.

Surface Plasmon Resonance Based Sensitive Immunosensor for Benzaldehyde Detection

2010 ◽  
Vol 130 (7) ◽  
pp. 269-274 ◽  
Author(s):  
Takeshi Onodera ◽  
Takuzo Shimizu ◽  
Norio Miura ◽  
Kiyoshi Matsumoto ◽  
Kiyoshi Toko
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance
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