scholarly journals Recognition of Chromosomal DNA inside Cells by Locked Nucleic Acids†

Biochemistry ◽  
2008 ◽  
Vol 47 (50) ◽  
pp. 13147-13149 ◽  
Author(s):  
Randall Beane ◽  
Sylvie Gabillet ◽  
Christophe Montaillier ◽  
Khalil Arar ◽  
David R. Corey
Keyword(s):  
Nucleic Acids ◽  
Chromosomal Dna ◽  
Locked Nucleic Acids

scholarly journals Inhibiting Gene Expression with Locked Nucleic Acids (LNAs) That Target Chromosomal DNA†

Biochemistry ◽  
2007 ◽  
Vol 46 (25) ◽  
pp. 7572-7580 ◽  
Author(s):  
Randall L. Beane ◽  
Rosalyn Ram ◽  
Sylvie Gabillet ◽  
Khalil Arar ◽  
Brett P. Monia ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nucleic Acids ◽  
Chromosomal Dna ◽  
Locked Nucleic Acids

scholarly journals Kinetics of DNA Strand Displacement Systems with Locked Nucleic Acids

2017 ◽  
Vol 121 (12) ◽  
pp. 2594-2602 ◽  
Author(s):  
Xiaoping Olson ◽  
Shohei Kotani ◽  
Bernard Yurke ◽  
Elton Graugnard ◽  
William L. Hughes
Keyword(s):  
Nucleic Acids ◽  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Locked Nucleic Acids ◽  
Dna Strand ◽  
Kinetics Of

scholarly journals Silencing Antibiotic Resistance with Antisense Oligonucleotides

Biomedicines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 416
Author(s):  
Saumya Jani ◽  
Maria Soledad Ramirez ◽  
Marcelo E. Tolmasky
Keyword(s):  
Antibiotic Resistance ◽  
Nucleic Acids ◽  
Antisense Oligonucleotides ◽  
Bacterial Infections ◽  
Genetic Disorders ◽  
Antibiotic Resistance Genes ◽  
Short Review ◽  
Rnase H ◽  
Biological Processes ◽  
Locked Nucleic Acids

Antisense technologies consist of the utilization of oligonucleotides or oligonucleotide analogs to interfere with undesirable biological processes, commonly through inhibition of expression of selected genes. This field holds a lot of promise for the treatment of a very diverse group of diseases including viral and bacterial infections, genetic disorders, and cancer. To date, drugs approved for utilization in clinics or in clinical trials target diseases other than bacterial infections. Although several groups and companies are working on different strategies, the application of antisense technologies to prokaryotes still lags with respect to those that target other human diseases. In those cases where the focus is on bacterial pathogens, a subset of the research is dedicated to produce antisense compounds that silence or reduce expression of antibiotic resistance genes. Therefore, these compounds will be adjuvants administered with the antibiotic to which they reduce resistance levels. A varied group of oligonucleotide analogs like phosphorothioate or phosphorodiamidate morpholino residues, as well as peptide nucleic acids, locked nucleic acids and bridge nucleic acids, the latter two in gapmer configuration, have been utilized to reduce resistance levels. The major mechanisms of inhibition include eliciting cleavage of the target mRNA by the host’s RNase H or RNase P, and steric hindrance. The different approaches targeting resistance to β-lactams include carbapenems, aminoglycosides, chloramphenicol, macrolides, and fluoroquinolones. The purpose of this short review is to summarize the attempts to develop antisense compounds that inhibit expression of resistance to antibiotics.

FISHing with locked nucleic acids (LNA): evaluation of different LNA/DNA mixmers

2003 ◽  
Vol 17 (4) ◽  
pp. 165-169 ◽  
Author(s):  
Asli N Silahtaroglu ◽  
Niels Tommerup ◽  
Henrik Vissing
Keyword(s):  
Nucleic Acids ◽  
Locked Nucleic Acids

Locked nucleic acids (LNA) and medical applications

2003 ◽  
Vol 10 (3-4) ◽  
pp. 325-334 ◽  
Author(s):  
Henrik Ørum ◽  
Andreas Wolter ◽  
Lars Kongsbak
Keyword(s):  
Nucleic Acids ◽  
Medical Applications ◽  
Locked Nucleic Acids

Increase in Hybridization Rates with Oligodeoxyribonucleotides Containing Locked Nucleic Acids

2006 ◽  
Vol 24 (2) ◽  
pp. 171-182 ◽  
Author(s):  
Thomas K. Ormond ◽  
Daniel Spear ◽  
Jacqueline Stoll ◽  
Megan A. Mackey ◽  
Pamela M. St. John
Keyword(s):  
Nucleic Acids ◽  
Locked Nucleic Acids

Enhanced Inhibition of Transcription Start by Targeting with 2′-OMe Pentaribonucleotides Comprising Locked Nucleic Acids and Intercalating Nucleic Acids

ChemBioChem ◽  
2005 ◽  
Vol 6 (7) ◽  
pp. 1181-1184 ◽  
Author(s):  
Vyacheslav V. Filichev ◽  
Birte Vester ◽  
Lykke H. Hansen ◽  
Mohammed T. Abdel Aal ◽  
B. Ravindra Babu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Transcription Start ◽  
Locked Nucleic Acids

scholarly journals Triple-Helical DNA in Drosophila Heterochromatin

Cells ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 227 ◽  
Author(s):  
Eduardo Gorab
Keyword(s):  
Nucleic Acids ◽  
Dna Sequences ◽  
Detection Methods ◽  
Triplex Dna ◽  
Thiazole Orange ◽  
Chromosomal Dna ◽  
Mitotic Chromosomes ◽  
Satellite Repeats

Polynucleotide chains obeying Watson-Crick pairing are apt to form non-canonical complexes such as triple-helical nucleic acids. From early characterization in vitro, their occurrence in vivo has been strengthened by increasing evidence, although most remain circumstantial particularly for triplex DNA. Here, different approaches were employed to specify triple-stranded DNA sequences in the Drosophila melanogaster chromosomes. Antibodies to triplex nucleic acids, previously characterized, bind to centromeric regions of mitotic chromosomes and also to the polytene section 59E of mutant strains carrying the brown dominant allele, indicating that AAGAG tandem satellite repeats are triplex-forming sequences. The satellite probe hybridized to AAGAG-containing regions omitting chromosomal DNA denaturation, as expected, for the intra-molecular triplex DNA formation model in which single-stranded DNA coexists with triplexes. In addition, Thiazole Orange, previously described as capable of reproducing results obtained by antibodies to triple-helical DNA, binds to AAGAG repeats in situ thus validating both detection methods. Unusual phenotype and nuclear structure exhibited by Drosophila correlate with the non-canonical conformation of tandem satellite arrays. From the approaches that lead to the identification of triple-helical DNA in chromosomes, facilities particularly provided by Thiazole Orange use may broaden the investigation on the occurrence of triplex DNA in eukaryotic genomes.

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