scholarly journals Translesion DNA Synthesis by Human DNA Polymerase η on Templates Containing a Pyrimidopurinone Deoxyguanosine Adduct, 3-(2′-Deoxy-β-d-erythro-pentofuranosyl)pyrimido-[1,2-a]purin-10(3H)-one†

Biochemistry ◽  
2009 ◽  
Vol 48 (2) ◽  
pp. 471-480 ◽  
Author(s):  
Jennifer B. Stafford ◽  
Robert L. Eoff ◽  
Albena Kozekova ◽  
Carmelo J. Rizzo ◽  
F. Peter Guengerich ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Translesion Dna Synthesis ◽  
Human Dna ◽  
Translesion Dna

scholarly journals N-terminal domains of human DNA polymerase lambda promote primer realignment during translesion DNA synthesis

DNA Repair ◽  
2014 ◽  
Vol 22 ◽  
pp. 41-52 ◽  
Author(s):  
David J. Taggart ◽  
Daniel M. Dayeh ◽  
Saul W. Fredrickson ◽  
Zucai Suo
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Translesion Dna Synthesis ◽  
Human Dna ◽  
Translesion Dna ◽  
Terminal Domains

In vivo evidence that phenylalanine 171 acts as a molecular brake for translesion DNA synthesis across benzo[a]pyrene DNA adducts by human DNA polymerase κ

DNA Repair ◽  
2014 ◽  
Vol 15 ◽  
pp. 21-28 ◽  
Author(s):  
Akira Sassa ◽  
Tetsuya Suzuki ◽  
Yuki Kanemaru ◽  
Naoko Niimi ◽  
Hirofumi Fujimoto ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Dna Adducts ◽  
Translesion Dna Synthesis ◽  
Human Dna ◽  
Translesion Dna ◽  
Molecular Brake

Protein interactions in T7 DNA replisome inhibit the bypass of abasic site by DNA polymerase

Mutagenesis ◽  
2019 ◽  
Author(s):  
Zhenyu Zou ◽  
Tingting Liang ◽  
Zhongyan Xu ◽  
Jiayu Xie ◽  
Shuming Zhang ◽  
...  
Keyword(s):  
Dna Replication ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
Protein Interactions ◽  
Accessory Proteins ◽  
Abasic Site ◽  
Traditional Concept ◽  
Translesion Dna Synthesis ◽  
Dna Lesion ◽  
Translesion Dna

Abstract Abasic site as a common DNA lesion blocks DNA replication and is highly mutagenic. Protein interactions in T7 DNA replisome facilitate DNA replication and translesion DNA synthesis. However, bypass of an abasic site by T7 DNA replisome has never been investigated. In this work, we used T7 DNA replisome and T7 DNA polymerase alone as two models to study DNA replication on encountering an abasic site. Relative to unmodified DNA, abasic site strongly inhibited primer extension and completely blocked strand-displacement DNA synthesis, due to the decreased fraction of enzyme–DNA productive complex and the reduced average extension rates. Moreover, abasic site at DNA fork inhibited the binding of DNA polymerase or helicase onto fork and the binding between polymerase and helicase at fork. Notably and unexpectedly, we found DNA polymerase alone bypassed an abasic site on primer/template (P/T) substrate more efficiently than did polymerase and helicase complex bypass it at fork. The presence of gp2.5 further inhibited the abasic site bypass at DNA fork. Kinetic analysis showed that this inhibition at fork relative to that on P/T was due to the decreased fraction of productive complex instead of the average extension rates. Therefore, we found that protein interactions in T7 DNA replisome inhibited the bypass of DNA lesion, different from all the traditional concept that protein interactions or accessory proteins always promote DNA replication and DNA damage bypass, providing new insights in translesion DNA synthesis performed by DNA replisome.

scholarly journals Mechanism of Translesion DNA Synthesis by DNA Polymerase II

1996 ◽  
Vol 271 (40) ◽  
pp. 24662-24669 ◽  
Author(s):  
Tamar Paz-Elizur ◽  
Masaru Takeshita ◽  
Myron Goodman ◽  
Michael O'Donnell ◽  
Zvi Livneh
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Translesion Dna Synthesis ◽  
Translesion Dna ◽  
Dna Polymerase Ii

scholarly journals In VitroGap-directed Translesion DNA Synthesis of an Abasic Site Involving Human DNA Polymerases ϵ, λ, and β

2011 ◽  
Vol 286 (37) ◽  
pp. 32094-32104 ◽  
Author(s):  
Giuseppe Villani ◽  
Ulrich Hubscher ◽  
Nadege Gironis ◽  
Sinikka Parkkinen ◽  
Helmut Pospiech ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerases ◽  
Abasic Site ◽  
Translesion Dna Synthesis ◽  
Human Dna ◽  
Translesion Dna
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