scholarly journals Conformation and Membrane Position of the Region Linking the Two C2 Domains in Synaptotagmin 1 by Site-Directed Spin Labeling†

Biochemistry ◽  
2008 ◽  
Vol 47 (47) ◽  
pp. 12380-12388 ◽  
Author(s):  
Hao Huang ◽  
David S. Cafiso
Keyword(s):  
Spin Labeling ◽  
C2 Domains ◽  
Synaptotagmin 1 ◽  
Site Directed Spin Labeling

scholarly journals Structure and Function of Synaptotagmin 1 C2 Domains as Determined by Site-Directed Spin Labeling

2011 ◽  
Vol 100 (3) ◽  
pp. 144a
Author(s):  
Dawn Z. Herrick ◽  
Weiwei Kuo ◽  
Jeffrey F. Ellena ◽  
David S. Cafiso
Keyword(s):  
Spin Labeling ◽  
Structure And Function ◽  
C2 Domains ◽  
Synaptotagmin 1 ◽  
Site Directed Spin Labeling ◽  
And Function

scholarly journals Phosphatidylinositol 4,5 bisphosphate controls the cis and trans interactions of synaptotagmin 1

2019 ◽  
Author(s):  
S.B. Nyenhuis ◽  
A. Thapa ◽  
D. S. Cafiso
Keyword(s):  
Neurotransmitter Release ◽  
Plasma Membranes ◽  
Full Length ◽  
Membrane Insertion ◽  
Membrane Interface ◽  
Vesicle Membrane ◽  
C2 Domains ◽  
Synaptotagmin 1 ◽  
Site Directed Spin Labeling ◽  
Cis And Trans

AbstractSynaptotagmin 1 acts as the Ca2+-sensor for synchronous neurotransmitter release; however, the mechanism by which it functions is not understood and is presently a topic of considerable interest. Here we describe measurements on full-length membrane reconstituted synaptotagmin 1 using site-directed spin labeling where we characterize the linker region as well as the cis (vesicle membrane) and trans (cytoplasmic membrane) binding of its two C2 domains. In the full-length protein, the C2A domain does not undergo membrane insertion in the absence of Ca2+; however, the C2B domain will bind to and penetrate in trans to a membrane containing phosphatidylinositol 4,5 bisphosphate (PIP2), even if phosphatidylserine (PS) is present in the cis membrane. In the presence of Ca2+, the Ca2+-binding loops of C2A and C2B both insert into the membrane interface; moreover, C2A preferentially inserts into PS containing bilayers and will bind in a cis configuration to membranes containing PS even if a PIP2 membrane is presented in trans. The data are consistent with a bridging activity for Syt1 where the two domains bind to opposing vesicle and plasma membranes. The failure of C2A to bind membranes in the absence of Ca2+ and the long unstructured segment linking C2A to the vesicle membrane indicates that synaptotagmin 1 could act to significantly shorten the vesicle-plasma membrane distance with increasing levels of Ca2+.

scholarly journals Correction: Braun, T., et al. Expanding the Genetic Code for Site-Directed Spin-Labeling. Int. J. Mol. Sci. 2019, 20, 373

2021 ◽  
Vol 22 (4) ◽  
pp. 1574
Author(s):  
Theresa Braun ◽  
Malte Drescher ◽  
Daniel Summerer
Keyword(s):  
Genetic Code ◽  
Spin Labeling ◽  
Site Directed Spin Labeling

The authors wish to make the following two corrections to this paper [...]

scholarly journals Phosphatidylinositol Phosphates as Co-activators of Ca2+ Binding to C2 Domains of Synaptotagmin 1

2006 ◽  
Vol 281 (23) ◽  
pp. 15845-15852 ◽  
Author(s):  
LiYi Li ◽  
Ok-Ho Shin ◽  
Jeong-Seop Rhee ◽  
Demet Araç ◽  
Jong-Cheol Rah ◽  
...  
Keyword(s):  
C2 Domains ◽  
Synaptotagmin 1 ◽  
Phosphatidylinositol Phosphates

Nucleotide-dependent displacement and dynamics of the α-1 helix in kinesin revealed by site-directed spin labeling EPR

2014 ◽  
Vol 443 (3) ◽  
pp. 911-916 ◽  
Author(s):  
Satoshi Yasuda ◽  
Takanori Yanagi ◽  
Masafumi D. Yamada ◽  
Shoji Ueki ◽  
Shinsaku Maruta ◽  
...  
Keyword(s):  
Spin Labeling ◽  
Site Directed Spin Labeling

scholarly journals Membrane Docking Geometry of GRP1 PH Domain Bound to a Target Lipid Bilayer: An EPR Site-Directed Spin-Labeling and Relaxation Study

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33640 ◽  
Author(s):  
Huai-Chun Chen ◽  
Brian P. Ziemba ◽  
Kyle E. Landgraf ◽  
John A. Corbin ◽  
Joseph J. Falke
Keyword(s):  
Lipid Bilayer ◽  
Spin Labeling ◽  
Ph Domain ◽  
Relaxation Study ◽  
Site Directed Spin Labeling
Sign in / Sign up

Export Citation Format

Share Document