scholarly journals Steric Constraint in the Primary Photoproduct of Sensory Rhodopsin II Is a Prerequisite for Light-Signal Transfer to HtrII†

Biochemistry ◽  
2008 ◽  
Vol 47 (23) ◽  
pp. 6208-6215 ◽  
Author(s):  
Motohiro Ito ◽  
Yuki Sudo ◽  
Yuji Furutani ◽  
Takashi Okitsu ◽  
Akimori Wada ◽  
...  
Keyword(s):  
Light Signal ◽  
Signal Transfer ◽  
Sensory Rhodopsin ◽  
Steric Constraint

scholarly journals The archaeal sensory rhodopsin II/transducer complex: a model for transmembrane signal transfer

FEBS Letters ◽  
2004 ◽  
Vol 564 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Johann P Klare ◽  
Valentin I Gordeliy ◽  
Jörg Labahn ◽  
Georg Büldt ◽  
Heinz-Jürgen Steinhoff ◽  
...  
Keyword(s):  
Signal Transfer ◽  
Sensory Rhodopsin

scholarly journals Active State of Sensory Rhodopsin II: Structural Determinants for Signal Transfer and Proton Pumping

2011 ◽  
Vol 412 (4) ◽  
pp. 591-600 ◽  
Author(s):  
Ivan Gushchin ◽  
Anastasia Reshetnyak ◽  
Valentin Borshchevskiy ◽  
Andrii Ishchenko ◽  
Ekaterina Round ◽  
...  
Keyword(s):  
Proton Pumping ◽  
Active State ◽  
Signal Transfer ◽  
Structural Determinants ◽  
Sensory Rhodopsin

scholarly journals Mutant Screen Distinguishes between Residues Necessary for Light-Signal Perception and Signal Transfer by Phytochrome B

PLoS Genetics ◽  
2008 ◽  
Vol 4 (8) ◽  
pp. e1000158 ◽  
Author(s):  
Yoshito Oka ◽  
Tomonao Matsushita ◽  
Nobuyoshi Mochizuki ◽  
Peter H. Quail ◽  
Akira Nagatani
Keyword(s):  
Light Signal ◽  
Phytochrome B ◽  
Signal Transfer ◽  
Mutant Screen ◽  
Signal Perception

scholarly journals Effects of Sensory Rhodopsin II Complexation with its Cognate Transducer HtrII on the Local Environment of Internal Water Molecules

2010 ◽  
Vol 98 (3) ◽  
pp. 287a
Author(s):  
Mikkel Jensen ◽  
Erica C. Saint Clair ◽  
Alan Gabel ◽  
Vladislav B. Bergo ◽  
Elena N. Spudich ◽  
...  
Keyword(s):  
Local Environment ◽  
Water Molecules ◽  
Sensory Rhodopsin ◽  
Internal Water

scholarly journals A Novel Thin-Film Technique to Improve Accuracy of Fluorescence-Based Estimates for Periphytic Biofilms

Water ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1464
Author(s):  
Leon Katona ◽  
Yvonne Vadeboncoeur ◽  
Christopher T. Nietch ◽  
Katie Hossler
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Light Attenuation ◽  
Pulse Amplitude ◽  
Light Signal ◽  
Photosynthetic Parameters ◽  
Pam Fluorometry ◽  
Attenuation Coefficients ◽  
Thin Film Technique ◽  
Relative Errors

Recent studies suggest that photophysiological parameters for intact substrates with depth (e.g., periphytic biofilms, microphytobenthos) are overestimated by pulse-amplitude modulated (PAM) fluorometry. This overestimation results from depth-integration effects, following the activation of deeper photosynthesizing layers by an attenuated light signal. To mitigate this error, we propose a novel slide-based thin-film technique in which fluorescence is measured on a vertically representative subsample of the biofilm, spread evenly on a microscope slide. We compared bias and precision for photosynthetic parameters estimated through conventional PAM fluorometry on intact biofilms and through our novel slide-based technique, both theoretically and empirically. Numerical simulations confirmed the consistent overestimation of key parameters for intact biofilms, with relative errors up to 145%, compared to, at most, 52% on thin films. Paired empirical observations likewise demonstrated that estimates based on intact biofilms were consistently higher (up to 248%, p<0.001) than estimates from thin films. Numerical simulation suggested greater precision with the slide-based technique for homogeneous biofilms, but potentially less precision for heterogeneous biofilms with improper subsampling. Our empirical comparison, however, demonstrated some improvement in precision with the slide-based technique (e.g., the coefficient of variation for the maximum electron transport rate was reduced 30%, p=0.009). We recommend the use of the slide-based technique, particularly for biofilms that are thick or have small light attenuation coefficients. Care should be taken, however, to obtain vertically representative subsamples of the biofilm for measurement.

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