Critical Role of Arginine 160 of the EutB Protein Subunit for Active Site Structure and Radical Catalysis in Coenzyme B12-Dependent Ethanolamine Ammonia-lyase†

Biochemistry ◽  
2008 ◽  
Vol 47 (20) ◽  
pp. 5523-5535 ◽  
Author(s):  
Li Sun ◽  
Olivia A. Groover ◽  
Jeffrey M. Canfield ◽  
Kurt Warncke
Keyword(s):  
Active Site ◽  
Critical Role ◽  
Coenzyme B12 ◽  
Protein Subunit ◽  
Site Structure ◽  
Active Site Structure ◽  
Radical Catalysis

Active site structure and catalytic mechanisms of human peroxidases

2006 ◽  
Vol 445 (2) ◽  
pp. 199-213 ◽  
Author(s):  
Paul G. Furtmüller ◽  
Martina Zederbauer ◽  
Walter Jantschko ◽  
Jutta Helm ◽  
Martin Bogner ◽  
...  
Keyword(s):  
Active Site ◽  
Site Structure ◽  
Active Site Structure ◽  
Catalytic Mechanisms

scholarly journals Iron-Imprinted Single-Atomic Site Catalyst-Based Nanoprobe for Detection of Hydrogen Peroxide in Living Cells

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Zhaoyuan Lyu ◽  
Shichao Ding ◽  
Maoyu Wang ◽  
Xiaoqing Pan ◽  
Zhenxing Feng ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Active Site ◽  
Single Atom ◽  
Site Structure ◽  
Atomic Site ◽  
Ion Imprinting ◽  
Active Site Structure ◽  
In Situ Detection ◽  
Colorimetric Biosensing

AbstractFe-based single-atomic site catalysts (SASCs), with the natural metalloproteases-like active site structure, have attracted widespread attention in biocatalysis and biosensing. Precisely, controlling the isolated single-atom Fe-N-C active site structure is crucial to improve the SASCs’ performance. In this work, we use a facile ion-imprinting method (IIM) to synthesize isolated Fe-N-C single-atomic site catalysts (IIM-Fe-SASC). With this method, the ion-imprinting process can precisely control ion at the atomic level and form numerous well-defined single-atomic Fe-N-C sites. The IIM-Fe-SASC shows better peroxidase-like activities than that of non-imprinted references. Due to its excellent properties, IIM-Fe-SASC is an ideal nanoprobe used in the colorimetric biosensing of hydrogen peroxide (H2O2). Using IIM-Fe-SASC as the nanoprobe, in situ detection of H2O2 generated from MDA-MB-231 cells has been successfully demonstrated with satisfactory sensitivity and specificity. This work opens a novel and easy route in designing advanced SASC and provides a sensitive tool for intracellular H2O2 detection.

Unveiling the critical role of active site interaction in single atom catalyst towards hydrogen evolution catalysis

Nano Energy ◽  
2022 ◽  
Vol 93 ◽  
pp. 106819
Author(s):  
Feng Li ◽  
Gao-Feng Han ◽  
Yunfei Bu ◽  
Shanshan Chen ◽  
Ishfaq Ahmad ◽  
...  
Keyword(s):  
Hydrogen Evolution ◽  
Active Site ◽  
Critical Role ◽  
Single Atom
Sign in / Sign up

Export Citation Format

Share Document