Ubiquitylation of Proliferating Cell Nuclear Antigen and Recruitment of Human DNA Polymerase η†

Biochemistry ◽  
2008 ◽  
Vol 47 (13) ◽  
pp. 4141-4150 ◽  
Author(s):  
Nana Nikolaishvili-Feinberg ◽  
G. Scott Jenkins ◽  
Kathleen R. Nevis ◽  
Dean P. Staus ◽  
Cameron O. Scarlett ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Human Dna ◽  
Proliferating Cell

scholarly journals Proliferating Cell Nuclear Antigen-dependent Coordination of the Biological Functions of Human DNA Polymerase ι

2004 ◽  
Vol 279 (46) ◽  
pp. 48360-48368 ◽  
Author(s):  
Antonio E. Vidal ◽  
Patricia Kannouche ◽  
Vladimir N. Podust ◽  
Wei Yang ◽  
Alan R. Lehmann ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Biological Functions ◽  
Human Dna ◽  
Proliferating Cell ◽  
Dna Polymerase Ι

scholarly journals Identification of PCNA interacting protein motifs in human DNA polymerase delta

2020 ◽  
Author(s):  
Prashant Khandagale ◽  
Shweta Thakur ◽  
Narottam Acharya
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Dna Polymerase Delta ◽  
Interacting Protein ◽  
Protein Motifs ◽  
Human Dna ◽  
Biochemical Studies ◽  
Processivity Factor ◽  
Proliferating Cell

AbstractDNA polymerase delta (Polδ) is a highly processive essential replicative DNA polymerase. In humans, Polδ holoenzyme consists of p125, p50, p68, and p12 subunits and recently, we have shown that p12 exists as a dimer. Extensive biochemical studies suggest that all the subunits of Polδ interact with the processivity factor proliferating cell nuclear antigen (PCNA) to carry out a pivotal role in genomic DNA replication. While PCNA interaction protein (PIP) motifs in p68, p50 and p12 have been mapped, the PIP in p125, the catalytic subunit of the holoenzyme, remains elusive. Therefore, in this study by using multiple approaches we have conclusively mapped a non-canonical PIP box from residues 999VGGLLAFA1008 in p125, which binds to inter domain-connecting loop of PCNA with high affinity. Collectively, including previous studies, we conclude that similar to S. cerevisiae Polδ, each of the human Polδ subunits possess motif to interact with PCNA and significantly contribute towards the processive nature of this replicative DNA polymerase.

scholarly journals Human DNA Polymerase β, but Not λ, Can Bypass a 2-Deoxyribonolactone Lesion Together with Proliferating Cell Nuclear Antigen

2012 ◽  
Vol 8 (2) ◽  
pp. 336-344 ◽  
Author(s):  
Emmanuele Crespan ◽  
Emanuela Pasi ◽  
Shuhei Imoto ◽  
Ulrich Hübscher ◽  
Marc M. Greenberg ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Dna Polymerase Β ◽  
Human Dna ◽  
Proliferating Cell

scholarly journals Identification of PCNA-interacting protein motifs in human DNA polymerase δ

2020 ◽  
Vol 40 (4) ◽  
Author(s):  
Prashant Khandagale ◽  
Shweta Thakur ◽  
Narottam Acharya
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Protein Motif ◽  
Interacting Protein ◽  
Protein Motifs ◽  
Dna Polymerase Δ ◽  
Human Dna ◽  
Processivity Factor ◽  
Proliferating Cell

Abstract DNA polymerase δ (Polδ) is a highly processive essential replicative DNA polymerase. In humans, the Polδ holoenzyme consists of p125, p50, p68 and p12 subunits and recently, we showed that the p12 subunit exists as a dimer. Extensive biochemical studies suggest that all the subunits of Polδ interact with the processivity factor proliferating cell nuclear antigen (PCNA) to carry out a pivotal role in genomic DNA replication. While PCNA-interacting protein motif (PIP) motifs in p68, p50 and p12 have been mapped, same in p125, the catalytic subunit of the holoenzyme, remains elusive. Therefore, in the present study by using multiple approaches we have conclusively mapped a non-canonical PIP motif from residues 999VGGLLAFA1008 in p125, which binds to the inter-domain-connecting loop (IDCL) of PCNA with high affinity. Collectively, including previous studies, we conclude that similar to Saccharomyces cerevisiae Polδ, each of the human Polδ subunits possesses motif to interact with PCNA and significantly contributes toward the processive nature of this replicative DNA polymerase.

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