Two Novel Monothiol Glutaredoxins fromSaccharomyces cerevisiaeProvide Further Insight into Iron-Sulfur Cluster Binding, Oligomerization, and Enzymatic Activity of Glutaredoxins†

Biochemistry ◽  
2008 ◽  
Vol 47 (5) ◽  
pp. 1452-1463 ◽  
Author(s):  
Nikola Mesecke ◽  
Sarah Mittler ◽  
Elisabeth Eckers ◽  
Johannes M. Herrmann ◽  
Marcel Deponte
Keyword(s):  
Enzymatic Activity ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Insight Into

scholarly journals An Iron-Sulfur Cluster in the C-terminal Domain of the p58 Subunit of Human DNA Primase

2007 ◽  
Vol 282 (46) ◽  
pp. 33444-33451 ◽  
Author(s):  
Brian E. Weiner ◽  
Hao Huang ◽  
Brian M. Dattilo ◽  
Mark J. Nilges ◽  
Ellen Fanning ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Dna Replication ◽  
Enzymatic Activity ◽  
Limited Proteolysis ◽  
High Potential ◽  
Multiple Sequence ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Dna Primase ◽  
Iron Sulfur

DNA primase synthesizes short RNA primers that are required to initiate DNA synthesis on the parental template strands during DNA replication. Eukaryotic primase contains two subunits, p48 and p58, and is normally tightly associated with DNA polymerase α. Despite the fundamental importance of primase in DNA replication, structural data on eukaryotic DNA primase are lacking. The p48/p58 dimer was subjected to limited proteolysis, which produced two stable structural domains: one containing the bulk of p48 and the other corresponding to the C-terminal fragment of p58. These domains were identified by mass spectrometry and N-terminal sequencing. The C-terminal p58 domain (p58C) was expressed, purified, and characterized. CD and NMR spectroscopy experiments demonstrated that p58C forms a well folded structure. The protein has a distinctive brownish color, and evidence from inductively coupled plasma mass spectrometry, UV-visible spectrophotometry, and EPR spectroscopy revealed characteristics consistent with the presence of a [4Fe-4S] high potential iron protein cluster. Four putative cysteine ligands were identified using a multiple sequence alignment, and substitution of just one was sufficient to cause loss of the iron-sulfur cluster and a reduction in primase enzymatic activity relative to the wild-type protein. The discovery of an iron-sulfur cluster in DNA primase that contributes to enzymatic activity provides the first suggestion that the DNA replication machinery may have redox-sensitive activities. Our results offer new horizons in which to investigate the function of high potential [4Fe-4S] clusters in DNA-processing machinery.

scholarly journals Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Nicholas G. Fox ◽  
Xiaodi Yu ◽  
Xidong Feng ◽  
Henry J. Bailey ◽  
Alain Martelli ◽  
...  
Keyword(s):  
Activation Mechanism ◽  
Cluster Assembly ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Insight Into

scholarly journals Iron–Sulfur Cluster Engineering Provides Insight into the Evolution of Substrate Specificity among Sulfonucleotide Reductases

2011 ◽  
Vol 7 (2) ◽  
pp. 306-315 ◽  
Author(s):  
Devayani P. Bhave ◽  
Jiyoung A. Hong ◽  
Rebecca L. Keller ◽  
Carsten Krebs ◽  
Kate S. Carroll
Keyword(s):  
Substrate Specificity ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Insight Into

Structural Insight into Poplar Glutaredoxin C1 with a Bridging Iron−Sulfur Cluster at the Active Site†,‡

Biochemistry ◽  
2006 ◽  
Vol 45 (26) ◽  
pp. 7998-8008 ◽  
Author(s):  
Yingang Feng ◽  
Nan Zhong ◽  
Nicolas Rouhier ◽  
Toshiharu Hase ◽  
Masami Kusunoki ◽  
...  
Keyword(s):  
Active Site ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Structural Insight ◽  
Insight Into

scholarly journals Identification of an Iron-Sulfur Cluster That Modulates the Enzymatic Activity in NarE, aNeisseria meningitidisADP-ribosyltransferase

2009 ◽  
Vol 284 (48) ◽  
pp. 33040-33047 ◽  
Author(s):  
Mariangela Del Vecchio ◽  
Rebecca Pogni ◽  
Maria Camilla Baratto ◽  
Angela Nobbs ◽  
Rino Rappuoli ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur
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