scholarly journals Peptide Conformation and Supramolecular Organization in Amylin Fibrils:  Constraints from Solid-State NMR†

Biochemistry ◽  
2007 ◽  
Vol 46 (47) ◽  
pp. 13505-13522 ◽  
Author(s):  
Sorin Luca ◽  
Wai-Ming Yau ◽  
Richard Leapman ◽  
Robert Tycko
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Peptide Conformation ◽  
Supramolecular Organization

scholarly journals DNP-supported solid-state NMR studies of 13C,15N,29Si-enriched biosilica of Cyclotella cryptica and Thalassiosira pseudonana

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Helena Leona Ehren ◽  
Felicitas Kolbe ◽  
Alessandra Lucini Paioni ◽  
Eike Brunner ◽  
Marc Baldus
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Nuclear Polarization ◽  
Thalassiosira Pseudonana ◽  
Supramolecular Organization ◽  
Structural Elements ◽  
Nmr Studies ◽  
Amine Nitrogen ◽  
Nmr Signals ◽  
Cyclotella Cryptica

AbstractSolid-state NMR spectroscopy represents a powerful method for the investigation of diatom biosilica but detailed studies regarding its chemical composition and structural organization can be prohibited by insufficient spectroscopic sensitivity. Here, we used two-dimensional (2D) Dynamic Nuclear Polarization (DNP)-supported solid-state NMR experiments to obtain information about the molecular composition and supramolecular organization of proteins and carbohydrates in 13C,15N,29Si-labeled biosilica of C. cryptica. As a reference, we conducted DNP experiments on isotope-labeled biosilica of T. pseudonana. DNP-enhancement factors for different NMR signals, and thus, for different organic compounds, provide information about the supramolecular architecture of the biosilica. In addition, DNP-supported heteronuclear nitrogen-carbon correlation experiments allowed us to prove the presence of different structural elements of long chain polyamines (LCPAs) and revealed the occurrence of amine-nitrogen moieties exhibiting a correlation with carbonyl carbons that may indicate cross-linking of LCPAs to proteins as previously seen in studies on proteins extracted from other diatoms.

scholarly journals DNP-Supported Solid-State NMR Studies of 13C,15N,29Si-Enriched Biosilica of Cyclotella Cryptica and Thalassiosira Pseudonana

2020 ◽  
Author(s):  
Helena Ehren ◽  
Felicitas Kolbe ◽  
Alesandra Lucini Paioni ◽  
Eike Brunner ◽  
Marc Baldus
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Nuclear Polarization ◽  
Thalassiosira Pseudonana ◽  
Supramolecular Organization ◽  
Powerful Method ◽  
Nmr Studies ◽  
Amine Nitrogen ◽  
Nmr Signals ◽  
Cyclotella Cryptica

Abstract Solid-state NMR spectroscopy represents a powerful method for the investigation of diatom biosilica but detailed studies regarding its chemical composition and structural organization can be prohibited by insufficient spectroscopic sensitivity. Here, we used two-dimensional (2D) Dynamic Nuclear Polarization (DNP)-supported solid-state NMR experiments to obtain information about the molecular composition and supramolecular organization of proteins and carbohydrates in13C,15N,29Si-labeled biosilica of C. cryptica. As a reference, we conducted DNP experiments on isotope-labeled biosilica of T. pseudonana. DNP-enhancement factors for different NMR signals, and thus, for different organic compounds, provide information about the supramolecular architecture of the biosilica. In addition, DNP-supported heteronuclear nitrogen-carbon correlation experiments allowed us to prove the presence of different LCPAs (long chain polyamines) and revealed the occurrence of amine-nitrogen moieties exhibiting a correlation with carbonyl carbons that may indicate cross-linking of LCPAs to proteins as previously seen in studies on proteins extracted from other diatoms.

scholarly journals Alamethicin Supramolecular Organization in Lipid Membranes from 19F Solid-State NMR

2016 ◽  
Vol 111 (11) ◽  
pp. 2450-2459 ◽  
Author(s):  
Evgeniy S. Salnikov ◽  
Jesus Raya ◽  
Marta De Zotti ◽  
Ekaterina Zaitseva ◽  
Cristina Peggion ◽  
...  
Keyword(s):  
Solid State ◽  
Lipid Membranes ◽  
Solid State Nmr ◽  
Supramolecular Organization

A useful tool for the elaboration of new solid-state NMR experiments: PULSAR

1995 ◽  
Vol 92 ◽  
pp. 1939-1942 ◽  
Author(s):  
JP Amoureux ◽  
C Fernandez ◽  
Y Dumazy
Keyword(s):  
Solid State ◽  
Solid State Nmr

Quantitative analysis by 13C solid-state NMR of eumelanins

1994 ◽  
Vol 91 ◽  
pp. 881-887 ◽  
Author(s):  
M Hervé ◽  
J Hirschinger ◽  
P Granger ◽  
P Gilard ◽  
N Goetz
Keyword(s):  
Quantitative Analysis ◽  
Solid State ◽  
Solid State Nmr
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