Molecular Cloning and Biochemical Characterization of VIM-12, a Novel Hybrid VIM-1/VIM-2 Metallo-β-lactamase from aKlebsiella pneumoniaeClinical Isolate, Reveal Atypical Substrate Specificity†

Biochemistry ◽  
2007 ◽  
Vol 46 (45) ◽  
pp. 13170-13178 ◽  
Author(s):  
Maria Kontou ◽  
Spyros Pournaras ◽  
Ioulia Kristo ◽  
Alexandros Ikonomidis ◽  
Antonios N. Maniatis ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Molecular Cloning ◽  
Biochemical Characterization

scholarly journals Biochemical Characterization of the DNA Substrate Specificity of Werner Syndrome Helicase

2002 ◽  
Vol 277 (26) ◽  
pp. 23236-23245 ◽  
Author(s):  
Robert M. Brosh ◽  
Juwaria Waheed ◽  
Joshua A. Sommers
Keyword(s):  
Substrate Specificity ◽  
Biochemical Characterization ◽  
Werner Syndrome ◽  
Dna Substrate

scholarly journals Origin and evolution of transporter substrate specificity within the NPF family

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Morten Egevang Jørgensen ◽  
Deyang Xu ◽  
Christoph Crocoll ◽  
Heidi Asschenfeldt Ernst ◽  
David Ramírez ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Biochemical Characterization ◽  
Biosynthetic Pathways ◽  
Cyanogenic Glucosides ◽  
Evolutionary Path ◽  
Substrate Specificities ◽  
Origin And Evolution ◽  
Glucosinolate Biosynthesis ◽  
Phylogenetic Lineage

Despite vast diversity in metabolites and the matching substrate specificity of their transporters, little is known about how evolution of transporter substrate specificities is linked to emergence of substrates via evolution of biosynthetic pathways. Transporter specificity towards the recently evolved glucosinolates characteristic of Brassicales is shown to evolve prior to emergence of glucosinolate biosynthesis. Furthermore, we show that glucosinolate transporters belonging to the ubiquitous NRT1/PTR FAMILY (NPF) likely evolved from transporters of the ancestral cyanogenic glucosides found across more than 2500 species outside of the Brassicales. Biochemical characterization of orthologs along the phylogenetic lineage from cassava to A. thaliana, suggests that alterations in the electrogenicity of the transporters accompanied changes in substrate specificity. Linking the evolutionary path of transporter substrate specificities to that of the biosynthetic pathways, exemplify how transporter substrate specificities originate and evolve as new biosynthesis pathways emerge.

Molecular cloning and biochemical characterization of a Tau class glutathione S-transferase from Pinus brutia Ten

Trees ◽  
2020 ◽  
Vol 34 (3) ◽  
pp. 835-843
Author(s):  
Meltem Alper ◽  
Elif Öztetik ◽  
M. Yaşar Kaya ◽  
Feray Köçkar
Keyword(s):  
Molecular Cloning ◽  
Biochemical Characterization ◽  
Glutathione S Transferase ◽  
Pinus Brutia

Molecular cloning and biochemical characterization of a receptor-like serine/threonine kinase from rice

1994 ◽  
Vol 26 (3) ◽  
pp. 791-803 ◽  
Author(s):  
Yan Zhao ◽  
Xin-Hua Feng ◽  
John C. Watson ◽  
Paul J. Bottino ◽  
Shain-Dow Kung
Keyword(s):  
Molecular Cloning ◽  
Biochemical Characterization ◽  
Serine Threonine Kinase ◽  
Threonine Kinase
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