Substrate Specificity of Homogeneous Monkeypox Virus Uracil-DNA Glycosylase†

Biochemistry ◽  
2007 ◽  
Vol 46 (42) ◽  
pp. 11874-11881 ◽  
Author(s):  
Sophie Duraffour ◽  
Alexander A. Ishchenko ◽  
Murat Saparbaev ◽  
Jean-Marc Crance ◽  
Daniel Garin
Keyword(s):  
Substrate Specificity ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase ◽  
Monkeypox Virus

scholarly journals Uracil DNA glycosylase (UDG) activities in Bradyrhizobium diazoefficiens: characterization of a new class of UDG with broad substrate specificity

2017 ◽  
Vol 45 (10) ◽  
pp. 5863-5876 ◽  
Author(s):  
Ullas Valiya Chembazhi ◽  
Vinod Vikas Patil ◽  
Shivjee Sah ◽  
Wayne Reeve ◽  
Ravi P. Tiwari ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase ◽  
Broad Substrate Specificity ◽  
New Class

scholarly journals Uracil-DNA glycosylases preferentially excise mispaired uracil

1992 ◽  
Vol 287 (3) ◽  
pp. 1007-1010 ◽  
Author(s):  
A Verri ◽  
P Mazzarello ◽  
S Spadari ◽  
F Focher
Keyword(s):  
Substrate Specificity ◽  
Dna Glycosylase ◽  
Complementary Strand ◽  
Dna Glycosylases ◽  
Proliferating Cells ◽  
Uracil Dna Glycosylase ◽  
Cytosine Deamination ◽  
Efficient Recognition ◽  
Single Stranded Oligonucleotide

We have investigated the substrate specificity of human, viral and bacterial uracil-DNA glycosylases employing as substrate double-stranded oligonucleotides containing in the same position of the 5′-32P-labelled strand an uracil residue facing, on the complementary strand, guanine (mimicking cytosine deamination) or adenine (mimicking dUTP misincorporation). The enzyme removal of uracil was monitored and quantified by the generation of alkali-sensitive apyrimidinic sites. All three uracil-DNA glycosylases excise uracil from mispaired oligonucleotides (U/G) more efficiently than from paired oligonucleotides (U/A). The enzymes also remove uracil from single-stranded oligonucleotide with an efficiency similar to that observed with U/A paired oligonucleotide. The efficient recognition of U/G mispair by uracil-DNA glycosylase is important in minimizing miscoding transcripts and C/G-->T/A transitions in proliferating cells.

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