scholarly journals Quantitative Characterization of Weak Self-Association in Concentrated Solutions of Immunoglobulin G via the Measurement of Sedimentation Equilibrium and Osmotic Pressure†

Biochemistry ◽  
2007 ◽  
Vol 46 (28) ◽  
pp. 8373-8378 ◽  
Author(s):  
Mercedes Jiménez ◽  
Germán Rivas ◽  
Allen P. Minton
Keyword(s):  
Osmotic Pressure ◽  
Immunoglobulin G ◽  
Sedimentation Equilibrium ◽  
Concentrated Solutions ◽  
Quantitative Characterization ◽  
Self Association

Tracer sedimentation equilibrium: a powerful tool for the quantitative characterization of macromolecular self- and hetero-associations in solution

2003 ◽  
Vol 31 (5) ◽  
pp. 1015-1019 ◽  
Author(s):  
G. Rivas ◽  
A.P. Minton
Keyword(s):  
Concentration Gradient ◽  
Sedimentation Equilibrium ◽  
Concentrated Solutions ◽  
Solution Composition ◽  
Quantitative Characterization ◽  
Measurement And Analysis ◽  
Solute Interactions ◽  
New Applications ◽  
Solute Component

During the last two decades the measurement and analysis of sedimentation equilibrium has been used increasingly as a method for characterizing intermolecular interactions in solution. More recently, a variant of this technique has been developed, in which a trace amount of a single solute component is labelled so that the concentration gradient of that component at sedimentation equilibrium may be measured independently of the gradients of all other components. The dependence of equilibrium tracer gradients upon solution composition may be readily interpreted in the context of models for self- and hetero-association of the labelled component, and, in the case of concentrated solutions, repulsive as well as attractive solute–solute interactions. We present a summary of experimental and analytic methods, a brief review of some previously published applications, and a preview of new applications demonstrating capabilities beyond those afforded by other current techniques for characterizing macromolecular associations in solution.

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