Role of Gene 10 Protein in the Hierarchical Assembly of the Bacteriophage P22 Portal Vertex Structure†

Biochemistry ◽  
2007 ◽  
Vol 46 (30) ◽  
pp. 8776-8784 ◽  
Author(s):  
Adam S. Olia ◽  
Anshul Bhardwaj ◽  
Lisa Joss ◽  
Sherwood Casjens ◽  
Gino Cingolani
2020 ◽  
Vol 501 (2) ◽  
pp. 2210-2230
Author(s):  
Nhut Truong ◽  
Annalisa Pillepich ◽  
Norbert Werner

ABSTRACT Recent X-ray observations have revealed remarkable correlations between the masses of central supermassive black holes (SMBHs) and the X-ray properties of the hot atmospheres permeating their host galaxies, thereby indicating the crucial role of the atmospheric gas in tracing SMBH growth in the high-mass regime. We examine this topic theoretically using the IllustrisTNG cosmological simulations and provide insights to the nature of this SMBH – gaseous halo connection. By carrying out a mock X-ray analysis for a mass-selected sample of TNG100 simulated galaxies at $z$ = 0, we inspect the relationship between the masses of SMBHs and the hot gas temperatures and luminosities at various spatial and halo scales – from galactic (∼Re) to group/cluster scales (∼R500c). We find strong SMBH-X-ray correlations mostly in quenched galaxies and find that the correlations become stronger and tighter at larger radii. Critically, the X-ray temperature (kBTX) at large radii (r ≳ 5Re) traces the SMBH mass with a remarkably small scatter (∼0.2 dex). The relations emerging from IllustrisTNG are broadly consistent with those obtained from recent X-ray observations. Overall, our analysis suggests that, within the framework of IllustrisTNG, the present-time MBH–kBTX correlations at the high-mass end (MBH ≳ 108M⊙) are fundamentally a reflection of the SMBH mass–halo mass relation, which at such high masses is set by the hierarchical assembly of structures. The exact form, locus, and scatter of those scaling relations are, however, sensitive to feedback processes such as those driven by star formation and SMBH activity.


2007 ◽  
Vol 189 (14) ◽  
pp. 5276-5283 ◽  
Author(s):  
Yvonne M. Lee ◽  
Karen W. Dodson ◽  
Scott J. Hultgren

ABSTRACT P-pilus biogenesis occurs via the highly conserved chaperone-usher pathway and involves the strict coordination of multiple subunit proteins. All nonadhesin structural P-pilus subunits possess the same topology, consisting of two domains: an incomplete immunoglobulin-like fold (pilin body) and an N-terminal extension. Pilus subunits form interactions with one another through donor strand exchange, occurring at the usher, in which the N-terminal extension of an incoming subunit completes the pilin body of the preceding subunit, allowing the incorporation of the subunit into the pilus fiber. In this study, pilus subunits in which the N-terminal extension was either deleted or swapped with that of another subunit were used to examine the role of each domain of PapF in functions involving donor strand exchange and hierarchical assembly. We found that the N-terminal extension of PapF is required to adapt the PapG adhesin to the tip of the fiber. The pilin body of PapF is required to efficiently initiate assembly of the remainder of the pilus, with the assistance of the N-terminal extension. Thus, distinct functions were assigned to each region of the PapF subunit. In conclusion, all pilin subunits possess the same overall architectural topology; however, each N-terminal extension and pilin body has specific functions in pilus biogenesis.


2012 ◽  
Vol 287 (40) ◽  
pp. 33766-33780 ◽  
Author(s):  
G. Pauline Padilla-Meier ◽  
Eddie B. Gilcrease ◽  
Peter R. Weigele ◽  
Juliana R. Cortines ◽  
Molly Siegel ◽  
...  

Genetics ◽  
1995 ◽  
Vol 140 (2) ◽  
pp. 427-434 ◽  
Author(s):  
A Garzón ◽  
D A Cano ◽  
J Casadesús

Abstract In the absence of host RecA function, plasmid transduction by bacteriophage P22 can be mediated by Erf recombinase. Erf is not carried on the infecting particle but synthesized upon infection. In the recipient cell, Erf can promote both generalized plasmid transduction (which requires the circularization of plasmids transduced as linear multimers) and specialized plasmid transduction (which requires the release of plasmid DNA from linear plasmid-phage cointegrates). Both processes of Erf-mediated plasmid transduction require host RecBCD function. In contrast, RecBCD is not required for Erf-mediated circularization of P22 DNA.


2007 ◽  
Vol 3 (S245) ◽  
pp. 281-284
Author(s):  
Duncan A. Forbes ◽  
Lee Spitler

AbstractGlobular Clusters provide a unique method for tracing the formation and evolution of their host galaxies. As single stellar populations they are far easier to interpret than the multi-population complexity of galaxy field stars. The scaling properties of globular clusters provide important constraints on the hierarchical assembly history of galaxies. Here we briefly review recent progress using the Hubble Space Telescope for imaging and the Keck plus Gemini telescopes for spectroscopy. We argue that the red, or metal-rich, subpopulation of GCs is associated with the bulge/spheroid component of galaxies. As one of the oldest stellar systems available for study, we discuss how globular clusters can be used to constrain the formation of galaxy bulges, in particular the role of mergers vs secular evolution. We conclude that metal-rich GCs, and hence bulges, formed very early in the Universe with more recent mergers having a small effect at most.


Genetics ◽  
1997 ◽  
Vol 146 (2) ◽  
pp. 447-456 ◽  
Author(s):  
Brandon A Mann ◽  
James M Slauch

The generalized transducing bacteriophage of Salmonella typhimurium, P22, can transduce plasmids in addition to chromosomal markers. Previous studies have concentrated on transduction of pBR322 by P22 and P22HT, the high transducing mutant of P22. This study investigates the mechanism of P22HT transduction of low-copy number plasmids, namely pSC101 derivatives. We show that P22HT transduces pSC101 derivatives that share homology with the chromosome by two distinct mechanisms. In the first mechanism, the plasmid integrates into the chromosome of the donor by homologous recombination. This chromosomal fragment is then packaged in the transducing particle. The second mechanism is a size-dependent mechanism involving a putative plasmid multimer. We propose that this multimer is formed by interplasmidic recombination. In contrast, P22HT can efficiently transduce pBR322 by a third mechanism, which is independent of plasmid homology with the chromosome. It has been proposed that the phage packages a linear concatemer created during rolling circle replication of pBR322, similar in fashion to phage genome packaging. This study investigates the role of RecA, RecD, and RecF recombination proteins in plasmid/plasmid and plasmid/chromosome interactions that form packageable substrates in the donor. We also examine the resolution of various transduced plasmid species in the recipient and the roles of RecA and RecD in these processes.


1975 ◽  
Vol 91 (4) ◽  
pp. 439-462 ◽  
Author(s):  
David Botstein ◽  
Kenneth K. Lew ◽  
Virginia Jarvik ◽  
Charles A. Swanson
Keyword(s):  

2010 ◽  
Vol 77 (6) ◽  
pp. 1568-1582 ◽  
Author(s):  
Margaret M. Suhanovsky ◽  
Kristin N. Parent ◽  
Sarah E. Dunn ◽  
Timothy S. Baker ◽  
Carolyn M. Teschke

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