scholarly journals Spectroscopic Studies of the Salmonella enterica Adenosyltransferase Enzyme SeCobA: Molecular-Level Insight into the Mechanism of Substrate Cob(II)alamin Activation

Biochemistry ◽  
2014 ◽  
Vol 53 (50) ◽  
pp. 7969-7982 ◽  
Author(s):  
Ivan G. Pallares ◽  
Theodore C. Moore ◽  
Jorge C. Escalante-Semerena ◽  
Thomas C. Brunold
Keyword(s):  
Salmonella Enterica ◽  
Molecular Level ◽  
Spectroscopic Studies ◽  
Insight Into

scholarly journals Noni fruit’s water spot appearance on the second day of harvest: a trade-off between resistance and energy

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Tian Wu ◽  
Danyan Hu ◽  
Qingfen Wang
Keyword(s):  
Statistical Analysis ◽  
Molecular Level ◽  
Morinda Citrifolia ◽  
Transcriptome Data ◽  
Trade Off ◽  
Kegg Pathways ◽  
Available Energy ◽  
Climacteric Fruit ◽  
The Relationship ◽  
Insight Into

Abstract Background Noni (Morinda citrifolia Linn.) is a tropical tree that bears climacteric fruit. Previous observations and research have shown that the second day (2 d) after harvest is the most important demarcation point when the fruit has the same appearance as the freshly picked fruit (0 d); however, they are beginning to become water spot appearance. We performed a conjoint analysis of metabolome and transcriptome data for noni fruit of 0 d and 2 d to reveal what happened to the fruit at the molecular level. Genes and metabolites were annotated to KEGG pathways and the co-annotated KEGG pathways were used as a statistical analysis. Results We found 25 pathways that were significantly altered at both metabolic and transcriptional levels, including a total of 285 differentially expressed genes (DEGs) and 11 differential metabolites through an integrative analysis of transcriptomics and metabolomics. The energy metabolism and pathways originating from phenylalanine were disturbed the most. The upregulated resistance metabolites and genes implied the increase of resistance and energy consumption in the postharvest noni fruit. Most genes involved in glycolysis were downregulated, further limiting the available energy. This lack of energy led noni fruit to water spot appearance, a prelude to softening. The metabolites and genes related to the resistance and energy interacted and restricted each other to keep noni fruit seemingly hard within two days after harvest, but actually the softening was already unstoppable. Conclusions This study provides a new insight into the relationship between the metabolites and genes of noni fruit, as well as a foundation for further clarification of the post-ripening mechanism in noni fruit.

scholarly journals Salmonella enterica Subsp. houtenae Associated with an Abscess in Young Roe Deer (Capreolus capreolus)

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 654
Author(s):  
Adriana Trotta ◽  
Laura Del Sambro ◽  
Michela Galgano ◽  
Stefano Ciccarelli ◽  
Erika Ottone ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Epidemiological Data ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
Biochemical Identification ◽  
Salmonella Pathogenicity Islands ◽  
Insight Into

Background: S. enterica subsp. houtenae has been rarely documented, and very limited genomic information is available. This report describes a rare case of primary extraintestinal salmonellosis in a young roe deer, associated with Salmonella enterica subsp. houtenae. Methods: A traditional cultural-based analysis was carried out from the contents of a neck abscess; biochemical identification and PCR assay were performed to isolate and identify the pathogen. Through whole-genome sequencing (WGS), multilocus sequence typing (MLST), core genome MLST (cgMLST), and the Salmonella pathogenicity islands (SPIs) survey, resistome and virulome genes were investigated to gain insight into the virulence and antimicrobial resistance of S. houtenae. Results: Biochemical identification and PCR confirmed the presence of Salmonella spp. in the swelling. The WGS analysis identified Salmonella enterica subspecies houtenae serovar 43:z4,z23:- and ST 958. The virulence study predicted a multidrug resistance pattern with resistance shown against aminoglycosides, tetracycline, beta-lactamase, fluoroquinolones, fosfomycin, nitroimidazole, aminocoumarin, and peptide. Fifty-three antibiotic-resistant genes were identified. No plasmids were detected. Conclusion: This study demonstrates the importance of continuous surveillance of pathogenic salmonellae. Biomolecular analyses combined with epidemiological data can provide important information about poorly described Salmonella strains and can help to improve animal welfare.

scholarly journals R Coronae Borealis Stars: Long-Term Photometric & Spectroscopic Studies

1998 ◽  
Vol 11 (1) ◽  
pp. 379-379
Author(s):  
P.L. Cottrell ◽  
L. Skuljan ◽  
P.M. Kilmartin ◽  
C. Gilmore ◽  
W.A. Lawson
Keyword(s):  
Radial Velocity ◽  
Spectroscopic Studies ◽  
Photometric Data ◽  
Decline Phase ◽  
Dust Clouds ◽  
Medium Resolution ◽  
Formation And Evolution ◽  
R Coronae Borealis Stars ◽  
Insight Into

For more than a decade we have been able to acquire and analyse a significant amount of photometric data of the highly variable R Coronae Borealis (RCB) stars. This has made been possible by a photometric service observing programme instigated at the Observatory. These photometric data have been combined with less extensive spectroscopic coverage, particularly of the decline phase of these stars. These have been supplemented by observations obtained at Mount Stromlo and Siding Spring Observatories for a radial velocity study. Significantly more spectroscopic observations are now being acquired with the development of a new medium resolution spectrograph at Mount John University Observatory. In this poster we will present recent photometric and spectroscopic results for a number of the RCB stars in our sample. This observational and analysis work can be used to provide further insight into the nature of these stars, their likely progeny and progenitors and the processes that are involved in the formation and evolution of the obscuring dust clouds which cause the decline phase.

scholarly journals Cisplatin under oriented external electric fields: A deeper insight into electrochemotherapy at the molecular level

2020 ◽  
Author(s):  
Li Zhang ◽  
Ya‐Ling Ye ◽  
Xiao‐Ling Zhang ◽  
Xiang‐Hui Li ◽  
Qiao‐Hong Chen ◽  
...  
Keyword(s):  
Electric Fields ◽  
Molecular Level ◽  
External Electric Fields ◽  
Insight Into

scholarly journals Simulating Cross-Contamination of Cooked Pork with Salmonella enterica from Raw Pork through Home Kitchen Preparation in Vietnam

2018 ◽  
Vol 15 (10) ◽  
pp. 2324 ◽  
Author(s):  
Sinh Dang-Xuan ◽  
Hung Nguyen-Viet ◽  
Phuc Pham-Duc ◽  
Delia Grace ◽  
Fred Unger ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Household Survey ◽  
Cross Contamination ◽  
Food Handling ◽  
Transfer Rates ◽  
Handling Practices ◽  
Food Handling Practices ◽  
Cutting Boards ◽  
Insight Into ◽  
Pork Consumption

Pork is the most commonly consumed meat in Vietnam, and Salmonella enterica is a common contaminant. This study aimed to assess potential S. enterica cross-contamination between raw and cooked pork in Vietnamese households. Different scenarios for cross-contamination were constructed based on a household survey of pork handling practices (416 households). Overall, 71% of people used the same knife and cutting board for both raw and cooked pork; however, all washed their hands and utensils between handling raw and cooked pork. The different scenarios were experimentally tested. First, S. enterica was inoculated on raw pork and surfaces (hands, knives and cutting boards); next, water used for washing and pork were sampled to identify the presence and concentration of S. enterica during different scenarios of food preparation. Bootstrapping techniques were applied to simulate transfer rates of S. enterica cross-contamination. No cross-contamination to cooked pork was observed in the scenario of using the same hands with new cutting boards and knives. The probability of re-contamination in the scenarios involving re-using the cutting board after washing was significantly higher compared to the scenarios which used a new cutting board. Stochastic simulation found a high risk of cross-contamination from raw to cooked pork when the same hands, knives and cutting boards were used for handling raw and cooked pork (78%); when the same cutting board but a different knife was used, cross-contamination was still high (67%). Cross-contamination between was not seen when different cutting boards and knives were used for cutting raw and cooked pork. This study provided an insight into cross-contamination of S. enterica, given common food handling practices in Vietnamese households and can be used for risk assessment of pork consumption.

scholarly journals Molecular-Level Insight into How Hydroxyl Groups Boost Catalytic Activity in CO2 Hydrogenation into Methanol

Chem ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. 613-625 ◽  
Author(s):  
Yuhan Peng ◽  
Liangbing Wang ◽  
Qiquan Luo ◽  
Yun Cao ◽  
Yizhou Dai ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Molecular Level ◽  
Co2 Hydrogenation ◽  
Hydroxyl Groups ◽  
Insight Into

scholarly journals Insight into molecular-level interactions between imidazolium-based ionic liquids and cellulose combining NMR, SAXS and MD simulations

2019 ◽  
Vol 75 (a1) ◽  
pp. a268-a268
Author(s):  
Aparna Annamraju ◽  
Nicholas D. Smith ◽  
Loukas Petridis ◽  
Hugh O'Neill ◽  
Sai Venkatesh Pingali ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Molecular Level ◽  
Md Simulations ◽  
Insight Into

Wingless induces transdetermination in developing Drosophila imaginal discs

Development ◽  
1995 ◽  
Vol 121 (5) ◽  
pp. 1263-1272 ◽  
Author(s):  
L. Maves ◽  
G. Schubiger
Keyword(s):  
Molecular Level ◽  
Ectopic Expression ◽  
Imaginal Discs ◽  
Developmental Studies ◽  
New Approach ◽  
Cell Determination ◽  
Disc Cells ◽  
The Subject ◽  
Insight Into

Drosophila imaginal discs, the precursors of the adult fly appendages, have been the subject of intensive developmental studies, particularly on cell determination. Cultured disc fragments are recognized not only for the ability to maintain their determined state through extra cell divisions but also for the ability to transdetermine, or switch to the determined state of a different disc. An understanding of transdetermination at a molecular level will provide further insight into the requirements for maintaining cell determination. We find that ectopic expression of the Drosophila gene wingless induces transdetermination of foreleg imaginal disc cells to wing cells. This transdetermination occurs in foreleg discs of developing larvae without disc fragmentation. The in situ-transdetermining cells localize to the dorsal region of the foreleg disc. This wingless-induced transdetermination event is remarkably similar to the leg-to-wing switch that occurs after leg disc culture. Thus we have identified a new approach to a molecular dissection of transdetermination.

scholarly journals In SituStructures of Polar and Lateral Flagella Revealed by Cryo-Electron Tomography

2019 ◽  
Vol 201 (13) ◽  
Author(s):  
Shiwei Zhu ◽  
Maren Schniederberend ◽  
Daniel Zhitnitsky ◽  
Ruchi Jain ◽  
Jorge E. Galán ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Electron Tomography ◽  
Additional Insight ◽  
Content Type ◽  
Bacterial Flagellum ◽  
Cryo Electron Tomography ◽  
Serovar Typhimurium ◽  
Flagellar Assembly ◽  
Insight Into

ABSTRACTThe bacterial flagellum is a sophisticated self-assembling nanomachine responsible for motility in many bacterial pathogens, includingPseudomonas aeruginosa,Vibriospp., andSalmonella enterica. The bacterial flagellum has been studied extensively in the model systemsEscherichia coliandSalmonella entericaserovar Typhimurium, yet the range of variation in flagellar structure and assembly remains incompletely understood. Here, we used cryo-electron tomography and subtomogram averaging to determinein situstructures of polar flagella inP. aeruginosaand peritrichous flagella inS. Typhimurium, revealing notable differences between these two flagellar systems. Furthermore, we observed flagellar outer membrane complexes as well as many incomplete flagellar subassemblies, which provide additional insight into mechanisms underlying flagellar assembly and loss in bothP. aeruginosaandS. Typhimurium.IMPORTANCEThe bacterial flagellum has evolved as one of the most sophisticated self-assembled molecular machines, which confers locomotion and is often associated with virulence of bacterial pathogens. Variation in species-specific features of the flagellum, as well as in flagellar number and placement, results in structurally distinct flagella that appear to be adapted to the specific environments that bacteria encounter. Here, we used cutting-edge imaging techniques to determine high-resolutionin situstructures of polar flagella inPseudomonas aeruginosaand peritrichous flagella inSalmonella entericaserovar Typhimurium, demonstrating substantial variation between flagella in these organisms. Importantly, we observed novel flagellar subassemblies and provided additional insight into the structural basis of flagellar assembly and loss in bothP. aeruginosaandS. Typhimurium.

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