A New General Model for Predicting Melting Thermodynamics of Complementary and Mismatched B-Form Duplexes Containing Locked Nucleic Acids: Application to Probe Design for Digital PCR Detection of Somatic Mutations

Biochemistry ◽  
2015 ◽  
Vol 54 (6) ◽  
pp. 1338-1352 ◽  
Author(s):  
Curtis Hughesman ◽  
Kareem Fakhfakh ◽  
Roza Bidshahri ◽  
H. Louise Lund ◽  
Charles Haynes
Keyword(s):  
Nucleic Acids ◽  
General Model ◽  
Somatic Mutations ◽  
Digital Pcr ◽  
Pcr Detection ◽  
Probe Design ◽  
Locked Nucleic Acids ◽  
Melting Thermodynamics

scholarly journals Kinetics of DNA Strand Displacement Systems with Locked Nucleic Acids

2017 ◽  
Vol 121 (12) ◽  
pp. 2594-2602 ◽  
Author(s):  
Xiaoping Olson ◽  
Shohei Kotani ◽  
Bernard Yurke ◽  
Elton Graugnard ◽  
William L. Hughes
Keyword(s):  
Nucleic Acids ◽  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Locked Nucleic Acids ◽  
Dna Strand ◽  
Kinetics Of

scholarly journals Silencing Antibiotic Resistance with Antisense Oligonucleotides

Biomedicines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 416
Author(s):  
Saumya Jani ◽  
Maria Soledad Ramirez ◽  
Marcelo E. Tolmasky
Keyword(s):  
Antibiotic Resistance ◽  
Nucleic Acids ◽  
Antisense Oligonucleotides ◽  
Bacterial Infections ◽  
Genetic Disorders ◽  
Antibiotic Resistance Genes ◽  
Short Review ◽  
Rnase H ◽  
Biological Processes ◽  
Locked Nucleic Acids

Antisense technologies consist of the utilization of oligonucleotides or oligonucleotide analogs to interfere with undesirable biological processes, commonly through inhibition of expression of selected genes. This field holds a lot of promise for the treatment of a very diverse group of diseases including viral and bacterial infections, genetic disorders, and cancer. To date, drugs approved for utilization in clinics or in clinical trials target diseases other than bacterial infections. Although several groups and companies are working on different strategies, the application of antisense technologies to prokaryotes still lags with respect to those that target other human diseases. In those cases where the focus is on bacterial pathogens, a subset of the research is dedicated to produce antisense compounds that silence or reduce expression of antibiotic resistance genes. Therefore, these compounds will be adjuvants administered with the antibiotic to which they reduce resistance levels. A varied group of oligonucleotide analogs like phosphorothioate or phosphorodiamidate morpholino residues, as well as peptide nucleic acids, locked nucleic acids and bridge nucleic acids, the latter two in gapmer configuration, have been utilized to reduce resistance levels. The major mechanisms of inhibition include eliciting cleavage of the target mRNA by the host’s RNase H or RNase P, and steric hindrance. The different approaches targeting resistance to β-lactams include carbapenems, aminoglycosides, chloramphenicol, macrolides, and fluoroquinolones. The purpose of this short review is to summarize the attempts to develop antisense compounds that inhibit expression of resistance to antibiotics.

FISHing with locked nucleic acids (LNA): evaluation of different LNA/DNA mixmers

2003 ◽  
Vol 17 (4) ◽  
pp. 165-169 ◽  
Author(s):  
Asli N Silahtaroglu ◽  
Niels Tommerup ◽  
Henrik Vissing
Keyword(s):  
Nucleic Acids ◽  
Locked Nucleic Acids

Droplet digital PCR as an emerging tool in detecting pathogens nucleic acids in infectious diseases

2021 ◽  
Author(s):  
Biao Chen ◽  
Yufeng Jiang ◽  
Xiaohua Cao ◽  
Chen Liu ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
Nucleic Acids ◽  
Digital Pcr ◽  
Droplet Digital Pcr

Locked nucleic acids (LNA) and medical applications

2003 ◽  
Vol 10 (3-4) ◽  
pp. 325-334 ◽  
Author(s):  
Henrik Ørum ◽  
Andreas Wolter ◽  
Lars Kongsbak
Keyword(s):  
Nucleic Acids ◽  
Medical Applications ◽  
Locked Nucleic Acids

Increase in Hybridization Rates with Oligodeoxyribonucleotides Containing Locked Nucleic Acids

2006 ◽  
Vol 24 (2) ◽  
pp. 171-182 ◽  
Author(s):  
Thomas K. Ormond ◽  
Daniel Spear ◽  
Jacqueline Stoll ◽  
Megan A. Mackey ◽  
Pamela M. St. John
Keyword(s):  
Nucleic Acids ◽  
Locked Nucleic Acids

Enhanced Inhibition of Transcription Start by Targeting with 2′-OMe Pentaribonucleotides Comprising Locked Nucleic Acids and Intercalating Nucleic Acids

ChemBioChem ◽  
2005 ◽  
Vol 6 (7) ◽  
pp. 1181-1184 ◽  
Author(s):  
Vyacheslav V. Filichev ◽  
Birte Vester ◽  
Lykke H. Hansen ◽  
Mohammed T. Abdel Aal ◽  
B. Ravindra Babu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Transcription Start ◽  
Locked Nucleic Acids

scholarly journals Discovery of herpesviruses in multi-infected primates using locked nucleic acids (LNA) and a bigenic PCR approach

2007 ◽  
Vol 4 (1) ◽  
pp. 84 ◽  
Author(s):  
Sandra Prepens ◽  
Karl-Anton Kreuzer ◽  
Fabian Leendertz ◽  
Andreas Nitsche ◽  
Bernhard Ehlers
Keyword(s):  
Nucleic Acids ◽  
Locked Nucleic Acids

The first analogues of LNA (Locked Nucleic Acids): Phosphorothioate-LNA and 2′-thio-LNA

1998 ◽  
Vol 8 (16) ◽  
pp. 2219-2222 ◽  
Author(s):  
Ravindra Kumar ◽  
Sanjay K. Singh ◽  
Alexei A. Koshkin ◽  
Vivek K. Rajwanshi ◽  
Michael Meldgaard ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Locked Nucleic Acids
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