Hybridization of G-Quadruplex-Forming Peptide Nucleic Acids to Guanine-Rich DNA Templates Inhibits DNA Polymerase η Extension

Biochemistry ◽  
2014 ◽  
Vol 53 (32) ◽  
pp. 5315-5322 ◽  
Author(s):  
Connor T. Murphy ◽  
Anisha Gupta ◽  
Bruce A. Armitage ◽  
Patricia L. Opresko
Keyword(s):  
Nucleic Acids ◽  
Dna Polymerase ◽  
Peptide Nucleic Acids ◽  
Dna Templates ◽  
G Quadruplex

Targeting DNA G-Quadruplex Structures with Peptide Nucleic Acids

2012 ◽  
Vol 18 (14) ◽  
pp. 1984-1991 ◽  
Author(s):  
Igor G. Panyutin ◽  
Mykola I. Onyshchenko ◽  
Ethan A. Englund ◽  
Daniel H. Appella ◽  
Ronald D. Neumann
Keyword(s):  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
G Quadruplex

scholarly journals Topological impact of noncanonical DNA structures on Klenow fragment of DNA polymerase

2017 ◽  
Vol 114 (36) ◽  
pp. 9605-9610 ◽  
Author(s):  
Shuntaro Takahashi ◽  
John A. Brazier ◽  
Naoki Sugimoto
Keyword(s):  
Dna Polymerase ◽  
Mixed Type ◽  
Klenow Fragment ◽  
Polymorphic Region ◽  
Dna Templates ◽  
Dna Structures ◽  
Dna Polymerization ◽  
And Topology ◽  
G Quadruplex ◽  
The Stability

Noncanonical DNA structures that stall DNA replication can cause errors in genomic DNA. Here, we investigated how the noncanonical structures formed by sequences in genes associated with a number of diseases impacted DNA polymerization by the Klenow fragment of DNA polymerase. Replication of a DNA sequence forming an i-motif from a telomere, hypoxia-induced transcription factor, and an insulin-linked polymorphic region was effectively inhibited. On the other hand, replication of a mixed-type G-quadruplex (G4) from a telomere was less inhibited than that of the antiparallel type or parallel type. Interestingly, the i-motif was a better inhibitor of replication than were mixed-type G4s or hairpin structures, even though all had similar thermodynamic stabilities. These results indicate that both the stability and topology of structures formed in DNA templates impact the processivity of a DNA polymerase. This suggests that i-motif formation may trigger genomic instability by stalling the replication of DNA, causing intractable diseases.

Molecular insights on the dynamic stability of peptide nucleic acid functionalized carbon and boron nitride nanotubes

2021 ◽  
Vol 23 (1) ◽  
pp. 219-228
Author(s):  
Nabanita Saikia ◽  
Mohamed Taha ◽  
Ravindra Pandey
Keyword(s):  
Nucleic Acid ◽  
Boron Nitride ◽  
Nucleic Acids ◽  
Dynamic Stability ◽  
Peptide Nucleic Acid ◽  
Rational Design ◽  
Peptide Nucleic Acids ◽  
Boron Nitride Nanotubes ◽  
Molecular Hybrids ◽  
Single Wall Nanotubes

The rational design of self-assembled nanobio-molecular hybrids of peptide nucleic acids with single-wall nanotubes rely on understanding how biomolecules recognize and mediate intermolecular interactions with the nanomaterial's surface.

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