scholarly journals Epigenetic Modification, Dehydration, and Molecular Crowding Effects on the Thermodynamics of i-Motif Structure Formation from C-Rich DNA

Biochemistry ◽  
2014 ◽  
Vol 53 (10) ◽  
pp. 1586-1594 ◽  
Author(s):  
Yogini P. Bhavsar-Jog ◽  
Eric Van Dornshuld ◽  
Tracy A. Brooks ◽  
Gregory S. Tschumper ◽  
Randy M. Wadkins
Keyword(s):  
Structure Formation ◽  
Epigenetic Modification ◽  
Molecular Crowding ◽  
Crowding Effects

Two dimensional crowding effects on protein folding at interfaces observed by chiral vibrational sum frequency generation spectroscopy

2018 ◽  
Vol 20 (35) ◽  
pp. 22421-22426 ◽  
Author(s):  
Wei Liu ◽  
Li Fu ◽  
Zhuguang Wang ◽  
Zahra Sohrabpour ◽  
Xiaobai Li ◽  
...  
Keyword(s):  
Protein Folding ◽  
Sum Frequency Generation ◽  
Molecular Crowding ◽  
Two Dimensional ◽  
Sum Frequency ◽  
Crowding Effects ◽  
Sum Frequency Generation Spectroscopy ◽  
Frequency Generation

Vibrational sum frequency generation studies demonstrate the effects of two-dimensional molecular crowding on protein folding at interfaces.

scholarly journals The Effects of Molecular Crowding on the Structure and Stability of G-Quadruplexes with an Abasic Site

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Takeshi Fujimoto ◽  
Shu-ichi Nakano ◽  
Daisuke Miyoshi ◽  
Naoki Sugimoto
Keyword(s):  
Site Effects ◽  
Enthalpy Change ◽  
Molecular Crowding ◽  
Abasic Site ◽  
Structure And Stability ◽  
Quadruplex Structure ◽  
Abasic Sites ◽  
Crowding Effects ◽  
G Quadruplex ◽  
Quadruplex Formation

Both cellular environmental factors and chemical modifications critically affect the properties of nucleic acids. However, the structure and stability of DNA containing abasic sites under cell-mimicking molecular crowding conditions remain unclear. Here, we investigated the molecular crowding effects on the structure and stability of the G-quadruplexes including a single abasic site. Structural analysis by circular dichroism showed that molecular crowding by PEG200 did not affect the topology of the G-quadruplex structure with or without an abasic site. Thermodynamic analysis further demonstrated that the degree of stabilization of the G-quadruplex by molecular crowding decreased with substitution of an abasic site for a single guanine. Notably, we found that the molecular crowding effects on the enthalpy change for G-quadruplex formation had a linear relationship with the abasic site effects depending on its position. These results are useful for predicting the structure and stability of G-quadruplexes with abasic sites in the cell-mimicking conditions.

scholarly journals Molecular crowding effects on the biochemical properties of amyloid β–heme, Aβ–Cu and Aβ–heme–Cu complexes

2020 ◽  
Vol 11 (28) ◽  
pp. 7479-7486 ◽  
Author(s):  
Meng Li ◽  
Zhenqi Liu ◽  
Jinsong Ren ◽  
Xiaogang Qu
Keyword(s):  
Oxidative Damage ◽  
Amyloid Β ◽  
Biochemical Properties ◽  
Molecular Crowding ◽  
Crowding Effects ◽  
Cu Complexes

Our findings highlight the significance of hydration contributions to the interactions of Aβ–heme and Aβ–Cu as well as their peroxidase activities. Aβ40–Cu but not Aβ40–heme may play more important roles in the oxidative damage in the etiology of AD.

scholarly journals 2P180 Molecular crowding effects on intracellular mechanical environments(12. Cell biology,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S188
Author(s):  
Kenji Nishizawa ◽  
Kei Fujiwara ◽  
Miho Yanagisawa ◽  
Daisuke Mizuno
Keyword(s):  
Cell Biology ◽  
Molecular Crowding ◽  
Crowding Effects

Molecular crowding effects of linear polymers in protein solutions

2006 ◽  
Vol 119 (2) ◽  
pp. 186-195 ◽  
Author(s):  
Donald J. Winzor ◽  
Peter R. Wills
Keyword(s):  
Protein Solutions ◽  
Linear Polymers ◽  
Molecular Crowding ◽  
Crowding Effects
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