scholarly journals The N-Terminal Zinc Finger and Flanking Basic Domains Represent the Minimal Region of the Human Immunodeficiency Virus Type-1 Nucleocapsid Protein for Targeting Chaperone Function

Biochemistry ◽  
2013 ◽  
Vol 52 (46) ◽  
pp. 8226-8236 ◽  
Author(s):  
Mithun Mitra ◽  
Wei Wang ◽  
My-Nuong Vo ◽  
Ioulia Rouzina ◽  
George Barany ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Zinc Finger ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Chaperone Function ◽  
Immunodeficiency Virus ◽  
Minimal Region

scholarly journals Role of the N-Terminal Zinc Finger of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein in Virus Structure and Replication

1998 ◽  
Vol 72 (5) ◽  
pp. 4442-4447 ◽  
Author(s):  
Valérie Tanchou ◽  
Didier Decimo ◽  
Christine Péchoux ◽  
Daniela Lener ◽  
Véronique Rogemond ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Dna Synthesis ◽  
Zinc Finger ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Proviral Dna ◽  
Immunodeficiency Virus ◽  
Function Relationship

ABSTRACT Nucleocapsid protein (NCp7) of human immunodeficiency virus type 1 is found covering the genomic RNA in the interior of the viral particle. It is a highly basic protein with two zinc fingers of the form CX2CX4HX4C which exhibit strong affinity for a zinc cation. To study the structure-function relationship of the N-terminal zinc finger of NCp7, this domain was either deleted or changed to CX2CX4CX4C. We examined virus formation and structure as well as proviral DNA synthesis. Our data show that these two NC mutations result in the formation of particles with an abnormal core morphology and impair the end of proviral DNA synthesis, leading to noninfectious viruses.

scholarly journals Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Nuclear Localization Mediates Early Viral mRNA Expression

2002 ◽  
Vol 76 (20) ◽  
pp. 10444-10454 ◽  
Author(s):  
Jielin Zhang ◽  
Clyde S. Crumpacker
Keyword(s):  
Human Immunodeficiency Virus ◽  
Mrna Expression ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Viral Mrna ◽  
Genomic Rna ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT An important aspect of the pathophysiology of human immunodeficiency virus type 1 (HIV-1) infection is the ability of the virus to replicate in the host vigorously without a latent phase and to kill cells with a dynamic turnover of 1.8 × 109 cells/day and 10.3 × 109 virions/24 h. The transcription of HIV-1 RNA in acute infection occurs at two stages; the transcription of viral spliced mRNA occurs early, and the transcription of viral genomic RNA occurs later. The HIV-1 Tat protein is translated from the early spliced mRNA and is critical for HIV-1 genomic RNA expression. The cellular transcription factors are important for HIV-1 early spliced mRNA expression. In this study we show that virion nucleocapsid protein (NC) has a role in expression of HIV-1 early spliced mRNA. The HIV-1 NC migrates from the cytoplasm to the nucleus and accumulates in the nucleus at 18 h postinfection. Mutations on HIV-1 NC zinc fingers change the pattern of early viral spliced mRNA expression and result in a delayed expression of early viral mRNA in HIV-infected cells. This delayed HIV-1 early spliced mRNA expression occurs after proviral DNA has been integrated into the cellular genome, as shown by a quantitative integration assay. These results show that virion NC plays an important role in inducing HIV-1 early mRNA expression and contributes to the rapid viral replication that occurs during HIV-1 infection.

The Vpr Protein of Human Immunodeficiency Virus Type 1 Binds to Nucleocapsid Protein p7in Vitro

1996 ◽  
Vol 218 (1) ◽  
pp. 352-355 ◽  
Author(s):  
Ming-Shi Li ◽  
Guillermo Garcia-Asua ◽  
Uma Bhattacharyya ◽  
Paolo Mascagni ◽  
Brian M. Austen ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Immunodeficiency Virus

Identification of a binding site for the human immunodeficiency virus type 1 nucleocapsid protein

Peptides ◽  
1994 ◽  
pp. 956-957
Author(s):  
K. Sakaguchi ◽  
N. Zambrano ◽  
E. T. Baldwin ◽  
B. A. Shapiro ◽  
J. W. Erickson ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Binding Site ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Immunodeficiency Virus

scholarly journals Identification of a binding site for the human immunodeficiency virus type 1 nucleocapsid protein.

1993 ◽  
Vol 90 (11) ◽  
pp. 5219-5223 ◽  
Author(s):  
K. Sakaguchi ◽  
N. Zambrano ◽  
E. T. Baldwin ◽  
B. A. Shapiro ◽  
J. W. Erickson ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Binding Site ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Immunodeficiency Virus
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