Highly Conserved Base A55 of 16S Ribosomal RNA Is Important for the Elongation Cycle of Protein Synthesis

Biochemistry ◽  
2013 ◽  
Vol 52 (38) ◽  
pp. 6695-6701 ◽  
Author(s):  
Bhubanananda Sahu ◽  
Prashant K. Khade ◽  
Simpson Joseph
Keyword(s):  
Protein Synthesis ◽  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Elongation Cycle

Effect of point mutations in the decoding site (C1400) region of 16S ribosomal RNA on the ability of ribosomes to carry out individual steps of protein synthesis

Biochemistry ◽  
1989 ◽  
Vol 28 (3) ◽  
pp. 1012-1019 ◽  
Author(s):  
Robert Denman ◽  
Didier Negre ◽  
Philip R. Cunningham ◽  
Kelvin Nurse ◽  
John Colgan ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Ribosomal Rna ◽  
Point Mutations ◽  
16S Ribosomal Rna

scholarly journals Ribosomal RNA methylation by GidB is a capacitor for discrimination of mischarged tRNA

2021 ◽  
Author(s):  
Zhuo Bi ◽  
Hong-Wei Su ◽  
Jia-Yao Hong ◽  
Babak Javid
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
16S Rrna ◽  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Rna Methylation ◽  
Proof Reading ◽  
Cellular Pathways ◽  
Rrna Methylation

SummaryDespite redundant cellular pathways to minimize translational errors, errors in protein synthesis are common. Pathways and mechanisms to minimize errors are classified as pre-ribosomal or ribosomal. Pre-ribosomal pathways are primarily concerned with appropriate pairing of tRNAs with their cognate amino acid, whereas to date, ribosomal proof-reading has been thought to only be concerned with minimizing decoding errors, since it has been assumed that the ribosomal decoding centre is blind to mischarged tRNAs. Here, we identified that in mycobacteria, deletion of the 16S ribosomal RNA methyltransferase gidB led to increased discrimination of mischarged tRNAs. GidB deletion was necessary but not sufficient for reducing mistranslation due to misacylation. Discrimination only occurred in mycobacteria enriched from environments or genetic backgrounds with high rates of mistranslation. Our data suggest that mycobacterial ribosomes are capable of discriminating mischarged tRNAs and that 16S rRNA methylation by GidB may act as a capacitor for moderating translational error.

scholarly journals A functional pseudoknot in 16S ribosomal RNA.

1991 ◽  
Vol 10 (8) ◽  
pp. 2203-2214 ◽  
Author(s):  
T. Powers ◽  
H.F. Noller
Keyword(s):  
Ribosomal Rna ◽  
16S Ribosomal Rna

A Comparison Between Elastic Network Interpolation and MD Simulation of 16S Ribosomal RNA

2003 ◽  
Vol 21 (3) ◽  
pp. 395-405 ◽  
Author(s):  
Moon K. Kim ◽  
Wen Li ◽  
Bruce A. Shapiro ◽  
Gregory S. Chirikjian
Keyword(s):  
Ribosomal Rna ◽  
Md Simulation ◽  
16S Ribosomal Rna ◽  
Elastic Network

scholarly journals Detection of Alcaligenes chronic endophthalmitis mimicking noninfectious uveitis with 16S ribosomal RNA PCR

2021 ◽  
Author(s):  
Tina Felfeli ◽  
Felicia Tai ◽  
Peng Yan ◽  
Tony Mazzulli ◽  
Nupura K. Bakshi ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
16S Ribosomal Rna
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