scholarly journals Comparative Analysis of Homology Models of the Ah Receptor Ligand Binding Domain: Verification of Structure–Function Predictions by Site-Directed Mutagenesis of a Nonfunctional Receptor

Biochemistry ◽  
2013 ◽  
Vol 52 (4) ◽  
pp. 714-725 ◽  
Author(s):  
Domenico Fraccalvieri ◽  
Anatoly A. Soshilov ◽  
Sibel I. Karchner ◽  
Diana G. Franks ◽  
Alessandro Pandini ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Ligand Binding ◽  
Structure Function ◽  
Binding Domain ◽  
Ligand Binding Domain ◽  
Site Directed Mutagenesis ◽  
Ah Receptor ◽  
Directed Mutagenesis ◽  
Receptor Ligand ◽  
Homology Models

scholarly journals Detection of the TCDD Binding-Fingerprint within the Ah Receptor Ligand Binding Domain by Structurally Driven Mutagenesis and Functional Analysis

Biochemistry ◽  
2009 ◽  
Vol 48 (25) ◽  
pp. 5972-5983 ◽  
Author(s):  
Alessandro Pandini ◽  
Anatoly A. Soshilov ◽  
Yujuan Song ◽  
Jing Zhao ◽  
Laura Bonati ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Ligand Binding ◽  
Binding Domain ◽  
Ligand Binding Domain ◽  
Ah Receptor ◽  
Receptor Ligand

scholarly journals Modeling the binding of diverse ligands within the Ah receptor ligand binding domain

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sara Giani Tagliabue ◽  
Samantha C. Faber ◽  
Stefano Motta ◽  
Michael S. Denison ◽  
Laura Bonati
Keyword(s):  
Ligand Binding ◽  
Binding Domain ◽  
Ligand Binding Domain ◽  
Ah Receptor ◽  
Receptor Ligand

scholarly journals Analysis of the ligand-binding domain of human retinoic acid receptor alpha by site-directed mutagenesis.

1996 ◽  
Vol 16 (10) ◽  
pp. 5386-5392 ◽  
Author(s):  
F P Lamour ◽  
P Lardelli ◽  
C M Apfel
Keyword(s):  
Retinoic Acid ◽  
Ligand Binding ◽  
Electrostatic Interactions ◽  
Cysteine Residue ◽  
Binding Domain ◽  
Ligand Binding Domain ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Arginine Residues ◽  
Antagonist Binding

Three subtypes of retinoic acid receptors (RAR), termed RAR alpha, RAR beta, and RAR gamma, have been described. They are composed of different structural domains, including distinct domains for DNA and ligand binding. RARs specifically bind all-trans-retinoic acid (RA), 9-cis-RA, and retinoid analogs. In this study, we examined the functional role of cysteine and arginine residues in the ligand-binding domain of hRAR alpha (hRAR alpha-LBD, amino acids 154 to 462). All conserved cysteine and arginine residues in this domain were mutated by site-directed mutagenesis, and the mutant proteins were characterized by blocking reactions, ligand-binding experiments, transactivation assays, and protease mapping. Changes of any cysteine residue of the hRAR alpha-LBD had no significant influence on the binding of all-trans RA or 9-cis RA. Interestingly, residue C-235 is specifically important in antagonist binding. With respect to arginine residues, only the two single mutations of R-276 and R-394 to alanine showed a dramatic decrease of agonist and antagonist binding whereas the R272A mutation showed only a slight effect. For all other arginine mutations, no differences in affinity were detectable. The two mutations R217A and R294A caused an increased binding efficiency for antagonists but no change in agonist binding. From these results, we can conclude that electrostatic interactions of retinoids with the RAR alpha-LBD play a significant role in ligand binding. In addition, antagonists show distinctly different requirements for efficient binding, which may contribute to their interference in the ligand-inducible transactivation function of RAR alpha.

Simulations of the Estrogen Receptor Ligand-Binding Domain:  Affinity of Natural Ligands and Xenoestrogens

2000 ◽  
Vol 43 (24) ◽  
pp. 4594-4605 ◽  
Author(s):  
B. Chris Oostenbrink ◽  
Jed W. Pitera ◽  
Marola M. H. van Lipzig ◽  
John H. N. Meerman ◽  
Wilfred F. van Gunsteren
Keyword(s):  
Estrogen Receptor ◽  
Ligand Binding ◽  
Binding Domain ◽  
Ligand Binding Domain ◽  
Receptor Ligand ◽  
Natural Ligands ◽  
Estrogen Receptor Ligand
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