GTP Binding Leads to Narrowing of the Conformer Population While Preserving the Structure of the RNA Aptamer: A Site-Specific Time-Resolved Fluorescence Dynamics Study

Biochemistry ◽  
2012 ◽  
Vol 51 (46) ◽  
pp. 9260-9269 ◽  
Author(s):  
T. Sanjoy Singh ◽  
B. J. Rao ◽  
G. Krishnamoorthy
Keyword(s):  
Rna Aptamer ◽  
Specific Time ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Site Specific ◽  
Gtp Binding ◽  
A Site

A sensitive sandwich structure time-resolved fluorescence method for thrombin detection

2018 ◽  
Vol 10 (34) ◽  
pp. 4178-4182 ◽  
Author(s):  
Tao Wu ◽  
Yuanfu Zhang ◽  
Tingting Hou ◽  
Yinghong Zhang ◽  
Shuhao Wang
Keyword(s):  
Sandwich Structure ◽  
Fluorescence Assay ◽  
Fluorescence Method ◽  
Specific Time ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Human Thrombin

We designed a sensitive and specific time-resolved fluorescence assay for detection of human thrombin.

Site-specific time-resolved FRET reveals local variations in the unfolding mechanism in an apparently two-state protein unfolding transition

2018 ◽  
Vol 20 (5) ◽  
pp. 3216-3232 ◽  
Author(s):  
Sandhya Bhatia ◽  
G. Krishnamoorthy ◽  
Jayant B. Udgaonkar
Keyword(s):  
Protein Unfolding ◽  
Specific Time ◽  
Time Resolved ◽  
Site Specific ◽  
Equilibrium Unfolding ◽  
Α Helix ◽  
Β Sheet ◽  
Different Parts

Using multi-site time-resolved FRET, it is shown that equilibrium unfolding of monellin is not only heterogeneous, but that the degree of non-cooperativity differs between the sole α-helix and different parts of the β-sheet.

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