scholarly journals Insight into the Molecular Basis of Aromatic Polyketide Cyclization: Crystal Structure and in Vitro Characterization of WhiE-ORFVI

Biochemistry ◽  
2012 ◽  
Vol 51 (14) ◽  
pp. 3079-3091 ◽  
Author(s):  
Ming-Yue Lee ◽  
Brian D. Ames ◽  
Shiou-Chuan Tsai
Keyword(s):  
Crystal Structure ◽  
Molecular Basis ◽  
In Vitro Characterization ◽  
Insight Into

scholarly journals Three new active members of the I-OnuI family of homing endonucleases

2017 ◽  
Vol 63 (8) ◽  
pp. 671-681 ◽  
Author(s):  
Iman M. Bilto ◽  
Tuhin K. Guha ◽  
Alvan Wai ◽  
Georg Hausner
Keyword(s):  
Dna Recognition ◽  
Mitochondrial Genes ◽  
Homing Endonucleases ◽  
Recognition Sequence ◽  
In Vitro Characterization ◽  
Target Sites ◽  
New Locations ◽  
Insight Into

In vitro characterization of 3 LAGLIDADG-type homing endonucleases (HEs) (I-CcaI, I-CcaII, and I-AstI) that belong to the I-OnuI family showed that they are functional HEs that cleave their respective cognate target sites. These endonucleases are encoded within group ID introns and appear to be orthologues that have inserted into 3 different mitochondrial genes: rns, rnl, and cox3. The endonuclease activity of I-CcaI was tested using various substrates, and its minimum DNA recognition sequence was estimated to be 26 nt. This set of HEs may provide some insight into how these types of mobile elements can migrate into new locations. This study provides additional endonucleases that can be added to the catalog of currently available HEs that may have various biotechnology applications.

Asymmetric synthesis and in vivo/in vitro characterization of new hybrid anticonvulsants derived from (2,5-dioxopyrrolidin-1-yl)phenylacetamides

2021 ◽  
Vol 109 ◽  
pp. 104751
Author(s):  
Michał Abram ◽  
Anna Rapacz ◽  
Gniewomir Latacz ◽  
Bartłomiej Szulczyk ◽  
Justyna Kalinowska-Tłuścik ◽  
...  
Keyword(s):  
Asymmetric Synthesis ◽  
In Vitro Characterization
Sign in / Sign up

Export Citation Format

Share Document