scholarly journals The Enzyme Function Initiative

Biochemistry ◽  
2011 ◽  
Vol 50 (46) ◽  
pp. 9950-9962 ◽  
Author(s):  
John A. Gerlt ◽  
Karen N. Allen ◽  
Steven C. Almo ◽  
Richard N. Armstrong ◽  
Patricia C. Babbitt ◽  
...  
Keyword(s):  
Enzyme Function ◽  
Enzyme Function Initiative

scholarly journals Nano-evolution and protein-based enzymatic evolution predicts the novel types of natural product nanozyme of traditional Chinese medicine: cases of herbzymes of Taishan-Huangjing (Rhizoma polygonati) and Goji (Lycium chinense)

2021 ◽  
Author(s):  
Guldan Nazarbek ◽  
Aidana Kutzhanova ◽  
Lazzat Nurtay ◽  
Chenglin Mu ◽  
Bexultan Kazybay ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Natural Product ◽  
Enzyme Function ◽  
The Novel ◽  
Lycium Chinense ◽  
Natural Protein

Nanozyme and natural product-derived herbzyme have been identified in different enzyme types simulating the natural protein-based enzyme function. How to explore and predict enzyme types of novel nanozyme when synthesized...

scholarly journals Arg206Cys substitution in DNASE1L3 causes a defect in DNASE1L3 protein secretion that confers risk of systemic lupus erythematosus

2021 ◽  
pp. annrheumdis-2020-218810
Author(s):  
Latanya N Coke ◽  
Hongxiu Wen ◽  
Mary Comeau ◽  
Mustafa H Ghanem ◽  
Andrew Shih ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Protein Secretion ◽  
Lupus Erythematosus ◽  
Conditional Analysis ◽  
Hek293 Cells ◽  
Enzyme Function ◽  
European Ancestry ◽  
Risk Haplotype ◽  
Systemic Lupus ◽  
Risk Association

ObjectivesTo determine if the polymorphism encoding the Arg206Cys substitution in DNASE1L3 explains the association of the DNASE1L3/PXK gene locus with systemic lupus erythematosus (SLE) and to examine the effect of the Arg206Cys sequence change on DNASE1L3 protein function.MethodsConditional analysis for rs35677470 was performed on cases and controls with European ancestry from the SLE Immunochip study, and genotype and haplotype frequencies were compared. DNASE1L3 protein levels were measured in cells and supernatants of HEK293 cells and monocyte-derived dendritic cells expressing recombinant and endogenous 206Arg and 206Cys protein variants.ResultsConditional analysis on rs35677470 eliminated the SLE risk association signal for lead single-nucleotide polymorphisms (SNPs) rs180977001 and rs73081554, which are found to tag the same risk haplotype as rs35677470. The modest effect sizes of the SLE risk genotypes (heterozygous risk OR=1.14 and homozygous risk allele OR=1.68) suggest some DNASE1L3 endonuclease enzyme function is retained. An SLE protective signal in PXK (lead SNP rs11130643) remained following conditioning on rs35677470. The DNASE1L3 206Cys risk variant maintained enzymatic activity, but secretion of the artificial and endogenous DNASE1L3 206Cys protein was substantially reduced.ConclusionsSLE risk association in the DNASE1L3 locus is dependent on the missense SNP rs35677470, which confers a reduction in DNASE1L3 protein secretion but does not eliminate its DNase enzyme function.

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