scholarly journals Stereoselective Hydrolysis of Organophosphate Nerve Agents by the Bacterial Phosphotriesterase

Biochemistry ◽  
2010 ◽  
Vol 49 (37) ◽  
pp. 7978-7987 ◽  
Author(s):  
Ping-Chuan Tsai ◽  
Andrew Bigley ◽  
Yingchun Li ◽  
Eman Ghanem ◽  
C. Linn Cadieux ◽  
...  
Keyword(s):  
Nerve Agents ◽  
Stereoselective Hydrolysis ◽  
Hydrolysis Of

Cu2+-dependent stereoselective hydrolysis of a chiral organophosphonothioate insecticide for domestic mammals’ sera and its albumins

2021 ◽  
pp. 112408
Author(s):  
Elizabeth Undiano ◽  
Susana Chávez ◽  
Pedro Mederos ◽  
Marcela Ayala ◽  
Antonio Monroy-Noyola
Keyword(s):  
Stereoselective Hydrolysis ◽  
Hydrolysis Of

scholarly journals Catalytic activity and stereoselectivity of engineered phosphotriesterases towards structurally different nerve agents in vitro

2021 ◽  
Author(s):  
Anja Köhler ◽  
Benjamin Escher ◽  
Laura Job ◽  
Marianne Koller ◽  
Horst Thiermann ◽  
...  
Keyword(s):  
Plasma Clearance ◽  
Nerve Agents ◽  
Inhibition Assay ◽  
Ache Inhibition ◽  
Catalytic Activities ◽  
Chiral Lc ◽  
Hydrolysis Of ◽  
Medical Countermeasures

AbstractHighly toxic organophosphorus nerve agents, especially the extremely stable and persistent V-type agents such as VX, still pose a threat to the human population and require effective medical countermeasures. Engineered mutants of the Brevundimonas diminuta phosphotriesterase (BdPTE) exhibit enhanced catalytic activities and have demonstrated detoxification in animal models, however, substrate specificity and fast plasma clearance limit their medical applicability. To allow better assessment of their substrate profiles, we have thoroughly investigated the catalytic efficacies of five BdPTE mutants with 17 different nerve agents using an AChE inhibition assay. In addition, we studied one BdPTE version that was fused with structurally disordered PAS polypeptides to enable delayed plasma clearance and one bispecific BdPTE with broadened substrate spectrum composed of two functionally distinct subunits connected by a PAS linker. Measured kcat/KM values were as high as 6.5 and 1.5 × 108 M−1 min−1 with G- and V-agents, respectively. Furthermore, the stereoselective degradation of VX enantiomers by the PASylated BdPTE-4 and the bispecific BdPTE-7 were investigated by chiral LC–MS/MS, resulting in a several fold faster hydrolysis of the more toxic P(−) VX stereoisomer compared to P(+) VX. In conclusion, the newly developed enzymes BdPTE-4 and BdPTE-7 have shown high catalytic efficacy towards structurally different nerve agents and stereoselectivity towards the toxic P(−) VX enantiomer in vitro and offer promise for use as bioscavengers in vivo.

Autocatalytic Hydrolysis of V-Type Nerve Agents

1996 ◽  
Vol 61 (24) ◽  
pp. 8407-8413 ◽  
Author(s):  
Yu-Chu Yang ◽  
Linda L. Szafraniec ◽  
William T. Beaudry ◽  
Dennis K. Rohrbaugh ◽  
Lawrence R. Procell ◽  
...  
Keyword(s):  
Nerve Agents ◽  
Hydrolysis Of

scholarly journals Microbial Stereoselective Hydrolysis of (±)-cis,trans-Ethyl Chrysanthemate for Preparation of (²)-trans-Acid

1991 ◽  
Vol 55 (11) ◽  
pp. 2865-2870
Author(s):  
Satoshi Mitsuda ◽  
Ryohei Komaki ◽  
Hideo Hirohara ◽  
Shigeyasu Nabeshima
Keyword(s):  
Trans Acid ◽  
Stereoselective Hydrolysis ◽  
Hydrolysis Of

scholarly journals Chemical synthesis and enzymatic, stereoselective hydrolysis of a functionalized dihydropyrimidine for the synthesis of β-amino acids

AMB Express ◽  
2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Christin Slomka ◽  
Sabilla Zhong ◽  
Anna Fellinger ◽  
Ulrike Engel ◽  
Christoph Syldatk ◽  
...  
Keyword(s):  
Amino Acids ◽  
Chemical Synthesis ◽  
Stereoselective Hydrolysis ◽  
Hydrolysis Of

scholarly journals Stereoselective hydrolysis of epoxy‐eicosanoids by mammalian soluble epoxide hydrolases

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Cosima Schmidt ◽  
Robert Fischer ◽  
Marija Markovic ◽  
Christophe Morisseau ◽  
Bruce D. Hammock ◽  
...  
Keyword(s):  
Epoxide Hydrolases ◽  
Stereoselective Hydrolysis ◽  
Hydrolysis Of

scholarly journals Hydrolysis of nerve agents by model nucleophiles: A computational study

2008 ◽  
Vol 175 (1-3) ◽  
pp. 200-203 ◽  
Author(s):  
Jeremy M. Beck ◽  
Christopher M. Hadad
Keyword(s):  
Computational Study ◽  
Nerve Agents ◽  
Hydrolysis Of
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