scholarly journals Conserved Asp327 of Walker B Motif in the N-Terminal Nucleotide Binding Domain (NBD-1) of Cdr1p ofCandida albicansHas Acquired a New Role in ATP Hydrolysis†

Biochemistry ◽  
2006 ◽  
Vol 45 (49) ◽  
pp. 14726-14739 ◽  
Author(s):  
Versha Rai ◽  
Manisha Gaur ◽  
Sudhanshu Shukla ◽  
Suneet Shukla ◽  
Suresh V. Ambudkar ◽  
...  
Keyword(s):  
Atp Hydrolysis ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Nucleotide Binding Domain ◽  
Terminal Nucleotide

scholarly journals The Specialized Hsp70 (HscA) Interdomain Linker Binds to Its Nucleotide-Binding Domain and Stimulates ATP Hydrolysis in BothcisandtransConfigurations

Biochemistry ◽  
2014 ◽  
Vol 53 (46) ◽  
pp. 7148-7159 ◽  
Author(s):  
T. Reid Alderson ◽  
Jin Hae Kim ◽  
Kai Cai ◽  
Ronnie O. Frederick ◽  
Marco Tonelli ◽  
...  
Keyword(s):  
Atp Hydrolysis ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Nucleotide Binding Domain ◽  
Interdomain Linker

scholarly journals ATP Binding by Monarch-1/NLRP12 Is Critical for Its Inhibitory Function

2007 ◽  
Vol 28 (5) ◽  
pp. 1841-1850 ◽  
Author(s):  
Zhengmao Ye ◽  
John D. Lich ◽  
Chris B. Moore ◽  
Joseph A. Duncan ◽  
Kristi L. Williams ◽  
...  
Keyword(s):  
Atp Hydrolysis ◽  
Interleukin 1 ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Atp Binding ◽  
Nucleotide Binding Domain ◽  
Hairpin Rna ◽  
Inhibitory Function ◽  
Cytokines And Chemokines ◽  
Hydrolysis Activity

ABSTRACT The recently discovered nucleotide binding domain-leucine rich repeat (NLR) gene family is conserved from plants to mammals, and several members are associated with human autoinflammatory or immunodeficiency disorders. This family is defined by a central nucleotide binding domain that contains the highly conserved Walker A and Walker B motifs. Although the nucleotide binding domain is a defining feature of this family, it has not been extensively studied in its purified form. In this report, we show that purified Monarch-1/NLRP12, an NLR protein that negatively regulates NF-κB signaling, specifically binds ATP and exhibits ATP hydrolysis activity. Intact Walker A/B motifs are required for this activity. These motifs are also required for Monarch-1 to undergo self-oligomerization, Toll-like receptor- or CD40L-activated association with NF-κB-inducing kinase (NIK) and interleukin-1 receptor-associated kinase 1 (IRAK-1), degradation of NIK, and inhibition of IRAK-1 phosphorylation. The stable expression of a Walker A/B mutant in THP-1 monocytes results in increased production of proinflammatory cytokines and chemokines to an extent comparable to that in cells in which Monarch-1 is silenced via short hairpin RNA. The results of this study are consistent with a model wherein ATP binding regulates the anti-inflammatory activity of Monarch-1.

scholarly journals Mutations Define Cross-talk between the N-terminal Nucleotide-binding Domain and Transmembrane Helix-2 of the Yeast Multidrug Transporter Pdr5

2008 ◽  
Vol 283 (50) ◽  
pp. 35010-35022 ◽  
Author(s):  
Zuben E. Sauna ◽  
Sherry Supernavage Bohn ◽  
Robert Rutledge ◽  
Michael P. Dougherty ◽  
Susan Cronin ◽  
...  
Keyword(s):  
Cross Talk ◽  
Transmembrane Helix ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Multidrug Transporter ◽  
Nucleotide Binding Domain ◽  
Terminal Nucleotide

scholarly journals Kinetics of the ATP Hydrolysis Cycle of the Nucleotide-binding Domain of Mdl1 Studied by a Novel Site-specific Labeling Technique

2005 ◽  
Vol 281 (9) ◽  
pp. 5694-5701 ◽  
Author(s):  
Chris van der Does ◽  
Chiara Presenti ◽  
Katrin Schulze ◽  
Stephanie Dinkelaker ◽  
Robert Tampé
Keyword(s):  
Atp Hydrolysis ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Nucleotide Binding Domain ◽  
Site Specific ◽  
Kinetics Of

scholarly journals The ATP Hydrolysis Cycle of the Nucleotide-binding Domain of the Mitochondrial ATP-binding Cassette Transporter Mdl1p

2003 ◽  
Vol 278 (29) ◽  
pp. 26862-26869 ◽  
Author(s):  
Eva Janas ◽  
Matthias Hofacker ◽  
Min Chen ◽  
Simone Gompf ◽  
Chris van der Does ◽  
...  
Keyword(s):  
Atp Hydrolysis ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Atp Binding ◽  
Nucleotide Binding Domain ◽  
Atp Binding Cassette Transporter ◽  
Atp Binding Cassette
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