Heparin-Mediated Conformational Changes in Fibronectin Expose Vascular Endothelial Growth Factor Binding Sites†

Biochemistry ◽  
2006 ◽  
Vol 45 (34) ◽  
pp. 10319-10328 ◽  
Author(s):  
Maria Mitsi ◽  
Zhenning Hong ◽  
Catherine E. Costello ◽  
Matthew A. Nugent
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Conformational Changes ◽  
Binding Sites ◽  
Vascular Endothelial ◽  
Factor Binding ◽  
Endothelial Growth Factor

scholarly journals Receptors of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) in fetal and adult human kidney: localization and [125I]VEGF binding sites.

1998 ◽  
Vol 9 (6) ◽  
pp. 1032-1044 ◽  
Author(s):  
M Simon ◽  
W Röckl ◽  
C Hornig ◽  
E F Gröne ◽  
H Theis ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Binding Sites ◽  
Vegf Receptor ◽  
Vascular Endothelial ◽  
Placenta Growth Factor ◽  
Receptor Proteins ◽  
Adult Kidney ◽  
Endothelial Growth Factor

Vascular endothelial growth factor (VEGF) has an important function in renal vascular ontogenesis and is constitutively expressed in podocytes of the adult kidney. The ability of VEGF to be chemotactic for monocytes and to increase the activity of collagenase and plasminogen activator may have implications for renal development and renal disease. In humans, the cellular actions of VEGF depend on binding to two specific receptors: Flt-1 and KDR. The aims of this study were: (1) to localize VEGF receptor proteins in human renal ontogenesis; (2) to quantify VEGF binding in human fetal and adult kidney; and (3) to dissect the binding into its two known components: the KDR and Flt-1 receptors. The latter aim was achieved by competitive binding of VEGF and placenta growth factor-2, which only binds to Flt-1. Quantification of 125I-VEGF binding sites was performed by autoradiography and computerized densitometry. By double-label immunohistochemistry, VEGF receptor proteins were localized solely to endothelial cells of preglomerular vessels, glomeruli, and postglomerular vessels. In developing glomeruli, VEGF receptor protein appeared as soon as endothelial cells were positive for von Willebrand factor. Specific 125I-VEGF binding could be localized to renal arteries and veins, glomeruli, and the tubulointerstitial capillary network in different developmental stages. Affinity (Kd) of adult (aK) and fetal (fK) kidneys was: Kd: glomeruli 38.6 +/- 11.2 (aK, n = 5), 36.3 +/- 7.1 (fK, n = 5); cortical tubulointerstitium 19.4 +/- 2.6 (aK, n = 5), 11.6 +/- 7.0 (fK, n = 5) pmol. Placenta growth factor-2 displaced VEGF binding in all renal structures by approximately 60%. VEGF receptor proteins thus were found only in renal endothelial cells. A coexpression of both VEGF binding sites could be shown, with Flt-1 demonstrating the most abundant VEGF receptor binding sites in the kidney. These studies support the hypothesis of a function for VEGF in adult kidney that is independent of angiogenesis.

scholarly journals Anti‐bevacizumab idiotype antibody vaccination is effective in inducing vascular endothelial growth factor‐binding response, impairing tumor outgrowth

2016 ◽  
Vol 107 (4) ◽  
pp. 551-555 ◽  
Author(s):  
Jéssica de Souza Sanches ◽  
Rodrigo Barbosa de Aguiar ◽  
Carolina Bellini Parise ◽  
Juliana Mayumi Suzuki ◽  
Roger Chammas ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Vascular Endothelial ◽  
Factor Binding ◽  
Tumor Outgrowth ◽  
Endothelial Growth Factor
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