Complete Lipopolysaccharide ofPlesiomonas shigelloidesO74:H5 (Strain CNCTC 144/92). 2. Lipid A, Its Structural Variability, the Linkage to the Core Oligosaccharide, and the Biological Activity of the Lipopolysaccharide†,‡

Biochemistry ◽  
2006 ◽  
Vol 45 (35) ◽  
pp. 10434-10447 ◽  
Author(s):  
Jolanta Lukasiewicz ◽  
Monika Dzieciatkowska ◽  
Tomasz Niedziela ◽  
Wojciech Jachymek ◽  
Anna Augustyniuk ◽  
...  
Keyword(s):  
Biological Activity ◽  
Lipid A ◽  
Core Oligosaccharide ◽  
The Core ◽  
Structural Variability

scholarly journals The Activity of Lipid A and Core Components of Bacterial Lipopolysaccharides in the Prevention of the Hypersensitive Response in Pepper

1997 ◽  
Vol 10 (7) ◽  
pp. 926-928 ◽  
Author(s):  
Mari-Anne Newman ◽  
Michael J. Daniels ◽  
J. Maxwell Dow
Keyword(s):  
Hypersensitive Response ◽  
Xanthomonas Campestris ◽  
Lipid A ◽  
Enteric Bacteria ◽  
Core Oligosaccharide ◽  
The Core ◽  
Minimal Structure ◽  
Core Components ◽  
Pre Treatment ◽  
Bacterial Lipopolysaccharides

Pre-treatment of leaves of pepper (Capsicum annuum) with lipopolysaccharide (LPS) preparations from enteric bacteria and Xanthomonas campestris could prevent the hypersensitive response caused by an avirulent X. campestris strain. By use of a range of deep-rough mutants, the minimal structure in Salmonella LPS responsible for the elicitation of this effect was determined to be lipid A attached to a disaccharide of 2-keto-3-deoxyoctulosonate; lipid A alone and the free core oligosaccharide from a Salmonella Ra mutant were not effective. For Xanthomonas, the core oligosaccharide alone had activity although lipid A was not effective. The results suggest that pepper cells can recognize different structures within bacterial LPS to trigger alterations in plant response to avirulent pathogens.

scholarly journals Interactions of Pulmonary Collectins with Bordetella bronchiseptica and Bordetella pertussis Lipopolysaccharide Elucidate the Structural Basis of Their Antimicrobial Activities

2004 ◽  
Vol 72 (12) ◽  
pp. 7124-7130 ◽  
Author(s):  
Lyndsay M. Schaeffer ◽  
Francis X. McCormack ◽  
Huixing Wu ◽  
Alison A. Weiss
Keyword(s):  
Bordetella Pertussis ◽  
Lipid A ◽  
Antimicrobial Activities ◽  
Innate Immune ◽  
Bordetella Bronchiseptica ◽  
Structural Basis ◽  
Wild Type ◽  
Core Oligosaccharide ◽  
The Core ◽  
O Antigen

ABSTRACT Surfactant proteins A (SP-A) and D (SP-D) play an important role in the innate immune defenses of the respiratory tract. SP-A binds to the lipid A region of lipopolysaccharide (LPS), and SP-D binds to the core oligosaccharide region. Both proteins induce aggregation, act as opsonins for neutrophils and macrophages, and have direct antimicrobial activity. Bordetella pertussis LPS has a branched core structure and a nonrepeating terminal trisaccharide. Bordetella bronchiseptica LPS has the same structure, but lipid A is palmitoylated and there is a repeating O-antigen polysaccharide. The ability of SP-A and SP-D to agglutinate and permeabilize wild-type and LPS mutants of B. pertussis and B. bronchiseptica was examined. Previously, wild-type B. pertussis was shown to resist the effects of SP-A; however, LPS mutants lacking the terminal trisaccharide were susceptible to SP-A. In this study, SP-A was found to aggregate and permeabilize a B. bronchiseptica mutant lacking the terminal trisaccharide, while wild-type B. bronchiseptica and mutants lacking only the palmitoyl transferase or O antigen were resistant to SP-A. Wild-type B. pertussis and B. bronchiseptica were both resistant to SP-D; however, LPS mutants of either strain lacking the terminal trisaccharide were aggregated and permeabilized by SP-D. We conclude that the terminal trisaccharide protects Bordetella species from the bactericidal functions of SP-A and SP-D. The O antigen and palmitoylated lipid A of B. bronchiseptica play no role in this resistance.

The core oligosaccharide component from Mannheimia (Pasteurella) haemolytica serotype Al lipopolysaccharide contains L-glycero-D-manno- and D-glycero-D-manno-heptoses: Analysis of the structure and conformation by high-resolution NMR spectroscopy

2002 ◽  
Vol 80 (8) ◽  
pp. 949-963 ◽  
Author(s):  
Jean-Robert Brisson ◽  
Ellen Crawford ◽  
Dušan Uhrín ◽  
Nam Huan Khieu ◽  
Malcolm B Perry ◽  
...  
Keyword(s):  
Lipid A ◽  
Inner Core ◽  
Chemical Shifts ◽  
Coupling Constants ◽  
Pasteurella Haemolytica ◽  
Core Oligosaccharide ◽  
The Core ◽  
Proton Coupling ◽  
Structure Formula ◽  
Nmr Methods

Previous studies from our laboratory have indicated that the lipopolysaccharide (LPS) from Mannheimia haemolytica serotype A1 contains both L-glycero-D-manno-heptose and D-glycero-D-manno-heptose residues. NMR methods making use of 1D 1H selective excitation and 2D (1H, 13C) and (1H, 31P) heteronuclear experiments were used for the structural determination of the major core oligosaccharide components of the deacylated low-molecular-mass LPS obtained following sequential treatment with anhydrous hydrazine and aq KOH. The core oligosaccharide region was found to be composed of a branched octasaccharide linked to the deacylated lipid A moiety via a 3-deoxy-4-phospho-D-manno-oct-2-ulosonate residue having the structure,[Formula: see text]Heterogeneity was found to be present at several linkages. NMR methods were devised to distinguish between the diastereomeric forms of the heptose residues. Synthesized monosaccharides of L-D- and D-D-heptose were used as model compounds for analysis of the 1H and 13C NMR chemical shifts and proton coupling constants. Molecular modeling using a Monte Carlo method for conformational analysis of saccharides was used to determine the conformation of the inner core of the oligosaccharide and to establish the stereochemical relationships between the heptoses.Key words: LPS, NMR, conformation, oligosaccharide, heptose.

A lipopolysaccharide-specific bacteriophage for Aeromonas salmonicida

1983 ◽  
Vol 29 (10) ◽  
pp. 1458-1461 ◽  
Author(s):  
E. E. Ishiguro ◽  
Teresa Ainsworth ◽  
D. H. Shaw ◽  
W. W. Kay ◽  
T. J. Trust
Keyword(s):  
Cell Wall ◽  
Lipid A ◽  
Aeromonas Salmonicida ◽  
Receptor Activity ◽  
Core Oligosaccharide ◽  
The Core ◽  
Phage Adsorption ◽  
Specific Bacteriophage

Cell wall lipopolysaccharide (LPS) was identified as the receptor for the Aeromonas salmonicida bacteriophage strain 55R-1. Mutants of A. salmonicida resistant to phage 55R-1 were unable to adsorb phage 55R-1 and were shown to be defective in LPS structure. Purified A. salmonicida LPS inactivated phage 55R-1, but the O-polysaccharide and the core oligosaccharide portions of the LPS were ineffective. These results suggest that lipid A was required for receptor activity. Antibodies directed against LPS also inhibited phage adsorption.

The Lipopolysaccharides of Neisseria gonorrhoeae Colony Types 1 and 4

1975 ◽  
Vol 53 (5) ◽  
pp. 623-629 ◽  
Author(s):  
Malcolm B. Perry ◽  
Virginia Daoust ◽  
Benito B. Diena ◽  
Fraser E. Ashton ◽  
Rebecca Wallace
Keyword(s):  
Molecular Weight ◽  
Neisseria Gonorrhoeae ◽  
High Molecular Weight ◽  
Lipid A ◽  
Core Oligosaccharide ◽  
Colony Type ◽  
Mild Hydrolysis

The lipopolysaccharides (LPS) of strains of Neisseria gonorrhoeae grown in type 1 (T1) and 4 (T4) colony forms have been isolated. LPS from T4 colony type cells on mild hydrolysis gave a lipid A and a core oligosaccharide composed of 2-amino-2-deoxy-D-glucose, D-glucose, D-galactose, L-glycero-D-manno-heptose and 3-deoxy-D-manno-octulosonic acid that appeared to be common to all the strains examined. LPS from T1 colony type cells on mild hydrolysis gave a lipid A and high molecular weight O polysaccharides which showed considerable differences in glycose composition for each strain examined. In those strains examined, T4 cells appear to produce a common 'R' type LPS whereas T1 cells produce an 'S' type LPS with structurally different O polysaccharide structures which probably account for serologically differentiated strains of N. gonorrhoeae.

scholarly journals Methanesulphonic acid movement in solid ice cores

2004 ◽  
Vol 39 ◽  
pp. 540-544 ◽  
Author(s):  
Barbara T. Smith ◽  
Tas D. Van Ommen ◽  
Mark A. J. Curran
Keyword(s):  
Diffusion Coefficient ◽  
Biological Activity ◽  
Sea Ice ◽  
Diffusion Mechanism ◽  
Ice Core ◽  
Ice Cores ◽  
East Antarctica ◽  
The Core ◽  
Central Value

AbstractMethanesulphonic acid (MSA) is an important trace-ion constituent in ice cores, with connections to biological activity and sea-ice distribution. Post-depositional movement of MSA has been documented in firn, and this study investigates movement in solid ice by measuring variations in MSA distribution across several horizontal sections from an ice core after 14.5 years storage. The core used is from below the bubble close-off depth at Dome Summit South, Law Dome, East Antarctica. MSA concentration was studied at 3 and 0.5 cm resolution across the core widths. Its distribution was uniform through the core centres, but the outer 3 cm showed gradients in concentrations down to less than half of the central value at the core edge. This effect is consistent with diffusion to the surrounding air during its 14.5 year storage. The diffusion coefficient is calculated to be 2 ×10–13 m2 s–1, and the implications for the diffusion mechanism are discussed.

scholarly journals Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola

2004 ◽  
Vol 271 (23-24) ◽  
pp. 4968-4977 ◽  
Author(s):  
Evelina L. Zdorovenko ◽  
Evgeny Vinogradov ◽  
Galina M. Zdorovenko ◽  
Buko Lindner ◽  
Olga V. Bystrova ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Core Oligosaccharide ◽  
The Core
Sign in / Sign up

Export Citation Format

Share Document