Modulation of Cell-Cycle Regulatory Signaling Network by 2-Methoxyestradiol in Prostate Cancer Cells Is Mediated through Multiple Signal Transduction Pathways†

Biochemistry ◽  
2006 ◽  
Vol 45 (11) ◽  
pp. 3703-3713 ◽  
Author(s):  
Gibanananda Ray ◽  
Gopal Dhar ◽  
Peter J. Van Veldhuizen ◽  
Snigdha Banerjee ◽  
Neela K. Saxena ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Cycle ◽  
Signal Transduction ◽  
Cancer Cells ◽  
Signaling Network ◽  
Signal Transduction Pathways ◽  
Prostate Cancer Cells ◽  
Multiple Signal

The impact of altered annexin I protein levels on apoptosis and signal transduction pathways in prostate cancer cells

The Prostate ◽  
2006 ◽  
Vol 66 (13) ◽  
pp. 1413-1424 ◽  
Author(s):  
Chin-Hui Hsiang ◽  
Toshiyuki Tunoda ◽  
Young E. Whang ◽  
Darren R. Tyson ◽  
David K. Ornstein
Keyword(s):  
Prostate Cancer ◽  
Signal Transduction ◽  
Cancer Cells ◽  
Signal Transduction Pathways ◽  
Prostate Cancer Cells ◽  
Protein Levels ◽  
Annexin I ◽  
The Impact

Reserpine Induces Apoptosis and Cell Cycle Arrest in Hormone Independent Prostate Cancer Cells through Mitochondrial Membrane Potential Failure

2019 ◽  
Vol 18 (9) ◽  
pp. 1313-1322 ◽  
Author(s):  
Manjula Devi Ramamoorthy ◽  
Ashok Kumar ◽  
Mahesh Ayyavu ◽  
Kannan Narayanan Dhiraviam
Keyword(s):  
Prostate Cancer ◽  
Reactive Oxygen Species ◽  
Cell Cycle ◽  
Membrane Potential ◽  
Cancer Cells ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Prostate Cancer Cells

Background: Reserpine, an indole alkaloid commonly used for hypertension, is found in the roots of Rauwolfia serpentina. Although the root extract has been used for the treatment of cancer, the molecular mechanism of its anti-cancer activity on hormonal independent prostate cancer remains elusive. Methods: we evaluated the cytotoxicity of reserpine and other indole alkaloids, yohimbine and ajmaline on Prostate Cancer cells (PC3) using MTT assay. We investigated the mechanism of apoptosis using a combination of techniques including acridine orange/ethidium bromide staining, high content imaging of Annexin V-FITC staining, flow cytometric quantification of the mitochondrial membrane potential and Reactive Oxygen Species (ROS) and cell cycle analysis. Results: Our results indicate that reserpine inhibits DNA synthesis by arresting the cells at the G2 phase and showed all standard sequential features of apoptosis including, destabilization of mitochondrial membrane potential, reduced production of reactive oxygen species and DNA ladder formation. Our in silico analysis further confirmed that indeed reserpine docks to the catalytic cleft of anti-apoptotic proteins substantiating our results. Conclusion: Collectively, our findings suggest that reserpine can be a novel therapeutic agent for the treatment of androgen-independent prostate cancer.

scholarly journals Effect of artemisinin derivatives on apoptosis and cell cycle in prostate cancer cells

2010 ◽  
Vol 21 (4) ◽  
pp. 423-432 ◽  
Author(s):  
Colm Morrissey ◽  
Byron Gallis ◽  
Jeffrey W. Solazzi ◽  
Byung Ju Kim ◽  
Roman Gulati ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Cycle ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Artemisinin Derivatives
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