Conformation Coupled Enzyme Catalysis:  Single-Molecule and Transient Kinetics Investigation of Dihydrofolate Reductase†

Biochemistry ◽  
2005 ◽  
Vol 44 (51) ◽  
pp. 16835-16843 ◽  
Author(s):  
Nina M. Antikainen ◽  
R. Derike Smiley ◽  
Stephen J. Benkovic ◽  
Gordon G. Hammes
Keyword(s):  
Dihydrofolate Reductase ◽  
Single Molecule ◽  
Enzyme Catalysis ◽  
Transient Kinetics

scholarly journals Single-molecule and transient kinetics investigation of the interaction of dihydrofolate reductase with NADPH and dihydrofolate

2004 ◽  
Vol 101 (9) ◽  
pp. 2764-2769 ◽  
Author(s):  
Z. Zhang ◽  
P. T. R. Rajagopalan ◽  
T. Selzer ◽  
S. J. Benkovic ◽  
G. G. Hammes
Keyword(s):  
Dihydrofolate Reductase ◽  
Single Molecule ◽  
Transient Kinetics

Dihydrofolate Reductase as a Model for Enzyme Catalysis

2015 ◽  
Vol 4 (2) ◽  
pp. 77-86 ◽  
Author(s):  
Kevin Francis ◽  
Amnon Kohen
Keyword(s):  
Dihydrofolate Reductase ◽  
Enzyme Catalysis

scholarly journals Protein motions and dynamic effects in enzyme catalysis

2015 ◽  
Vol 17 (46) ◽  
pp. 30817-30827 ◽  
Author(s):  
Louis Y. P. Luk ◽  
E. Joel Loveridge ◽  
Rudolf K. Allemann
Keyword(s):  
Dihydrofolate Reductase ◽  
Enzyme Catalysis ◽  
Catalytic Cycle ◽  
Dynamic Effects ◽  
Dynamic Coupling ◽  
Protein Motions ◽  
Catalytic Power

While the full catalytic power of dihydrofolate reductase depends on finely tuning protein motions in each step of the catalytic cycle, dynamic coupling to the actual chemical step is detrimental to catalysis.

Exploring mechanism of enzyme catalysis by on-chip transient kinetics coupled with global data analysis and molecular modeling

Chem ◽  
2021 ◽  
Author(s):  
David Hess ◽  
Veronika Dockalova ◽  
Piia Kokkonen ◽  
David Bednar ◽  
Jiri Damborsky ◽  
...  
Keyword(s):  
Data Analysis ◽  
Molecular Modeling ◽  
Enzyme Catalysis ◽  
Transient Kinetics ◽  
On Chip ◽  
Global Data

scholarly journals Single Molecule Enzyme Catalysis: Steps towards Accurate Kinetic Schemes

2013 ◽  
Vol 104 (2) ◽  
pp. 372a
Author(s):  
Tatyana Terentyeva ◽  
Johan Hofkens ◽  
Chun-Biu Li ◽  
Kerstin Blank
Keyword(s):  
Single Molecule ◽  
Enzyme Catalysis ◽  
Kinetic Schemes

scholarly journals Subunit Interactions in Enzyme Catalysis. Effect of Interactions on Transient Kinetics

1974 ◽  
Vol 49 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Christian MOUTTET ◽  
Francis FOUCHIER ◽  
Joannes NARI ◽  
Jacques RICARD
Keyword(s):  
Enzyme Catalysis ◽  
Transient Kinetics ◽  
Subunit Interactions
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