Acidic Region Tyrosines Provide Access Points for Allosteric Activation of the Autoinhibited Vav1 Dbl Homology Domain†

Biochemistry ◽  
2005 ◽  
Vol 44 (46) ◽  
pp. 15257-15268 ◽  
Author(s):  
Gaya K. Amarasinghe ◽  
Michael K. Rosen
Keyword(s):  
Allosteric Activation ◽  
Access Points ◽  
Dbl Homology Domain ◽  
Acidic Region

Food and Religious Identities in the Venetian Inquisition, ca. 1560–ca. 1640

2021 ◽  
Vol 74 (1) ◽  
pp. 181-214
Author(s):  
Eleanor Barnett
Keyword(s):  
Identity Construction ◽  
Religious Identity ◽  
Lived Religion ◽  
Ordinary People ◽  
Religious Identities ◽  
Access Points ◽  
Counter Reformation ◽  
Eating Practices ◽  
The City ◽  
Insight Into

Through Venetian Inquisition trials relating to Protestantism, witchcraft, and Judaism, this article illuminates the centrality of food and eating practices to religious identity construction. The Holy Office used food to assert its model of post-Tridentine piety and the boundaries between Catholics and the non-Catholic populations in the city. These trial records concurrently act as access points to the experiences and beliefs—to the lived religion—of ordinary people living and working in Venice from 1560 to 1640. The article therefore offers new insight into the workings and impacts of the Counter-Reformation.

scholarly journals Overexpression of SIS2, which contains an extremely acidic region, increases the expression of SWI4, CLN1 and CLN2 in sit4 mutants.

Genetics ◽  
1995 ◽  
Vol 139 (1) ◽  
pp. 95-107 ◽  
Author(s):  
C J Di Como ◽  
R Bose ◽  
K T Arndt
Keyword(s):  
Growth Rate ◽  
Protein Phosphatase ◽  
Rnase A ◽  
Micrococcal Nuclease ◽  
Nuclear Fraction ◽  
High Copy Number Plasmid ◽  
Carboxyl Terminal ◽  
Rna Accumulation ◽  
Bud Initiation ◽  
Acidic Region

Abstract The Saccharomyces cerevisiae SIS2 gene was identified by its ability, when present on a high copy number plasmid, to increase dramatically the growth rate of sit4 mutants. SIT4 encodes a type 2A-related protein phosphatase that is required in late G1 for normal G1 cyclin expression and for bud initiation. Overexpression of SIS2, which contains an extremely acidic carboxyl terminal region, stimulated the rate of CLN1, CLN2, SWI4 and CLB5 expression in sit4 mutants. Also, overexpression of SIS2 in a CLN1 cln2 cln3 strain stimulated the growth rate and the rate of CLN1 and CLB5 RNA accumulation during late G1. The SIS2 protein fractionated with nuclei and was released from the nuclear fraction by treatment with either DNase I or micrococcal nuclease, but not by RNase A. This result, combined with the finding that overexpression of SIS2 is extremely to a strain containing lower than normal levels of histones H2A and H2B, suggests that SIS2 might function to stimulate transcription via an interaction with chromatin.

Dynamical Basis of Allosteric Activation for the Plasmodium falciparum Protein Kinase G

2021 ◽  
Author(s):  
Jinfeng Huang ◽  
Jung Ah Byun ◽  
Bryan VanSchouwen ◽  
Philipp Henning ◽  
Friedrich W. Herberg ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Protein Kinase ◽  
Protein Kinase G ◽  
Allosteric Activation ◽  
Falciparum Protein

scholarly journals Measuring the impact of an acute visiting scheme on emergency department attendances – a pre-post cohort design

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Axel Kaehne ◽  
Paula Keating
Keyword(s):  
Emergency Department ◽  
Service Use ◽  
Emergency Department Visits ◽  
Service Utilisation ◽  
Specific Patient ◽  
Access Points ◽  
Latent Demand ◽  
Frequent Attenders ◽  
Cohort Design ◽  
The Impact

Abstract Background Emergency department (ED) attendances are contributing to rising costs of the National Health Service (NHS) in England. Critically assessing the impact of new services to reduce emergency department use can be difficult as new services may create additional access points, unlocking latent demand. The study evaluated an Acute Visiting Scheme (AVS) in a primary care context. We asked if AVS reduces overall ED demand and whether or not it changed utilisation patterns for frequent attenders. Method The study used a pre post single cohort design. The impact of AVS on all-cause ED attendances was hypothesised as a substitution effect, where AVS duty doctor visits would replace emergency department visits. Primary outcome was frequency of ED attendances. End points were reduction of frequency of service use and increase of intervals between attendances by frequent attenders. Results ED attendances for AVS users rose by 47.6%. If AVS use was included, there was a more than fourfold increase of total service utilisation, amounting to 438.3%. It shows that AVS unlocked significant latent demand. However, there was some reduction in the frequency of ED attendances for some patients and an increase in time intervals between ED attendances for others. Conclusion The study demonstrates that careful analysis of patient utilisation can detect a differential impact of AVS on the use of ED. As the new service created additional access points for patients and hence introduces an element of choice, the new service is likely to unlock latent demand. This study illustrates that AVS may be most useful if targeted at specific patient groups who are most likely to benefit from the new service.

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