Structural Dynamics and Processing of Nucleic Acids Revealed by Single-Molecule Spectroscopy†

Biochemistry ◽  
2004 ◽  
Vol 43 (14) ◽  
pp. 4055-4063 ◽  
Author(s):  
Taekjip Ha
Keyword(s):  
Nucleic Acids ◽  
Structural Dynamics ◽  
Single Molecule ◽  
Single Molecule Spectroscopy

scholarly journals Decoding Circulating Nucleic Acids in Human Serum Using Microfluidic Single Molecule Spectroscopy

2010 ◽  
Vol 132 (16) ◽  
pp. 5793-5798 ◽  
Author(s):  
Kelvin J. Liu ◽  
Malcolm V. Brock ◽  
Ie-Ming Shih ◽  
Tza-Huei Wang
Keyword(s):  
Nucleic Acids ◽  
Human Serum ◽  
Single Molecule ◽  
Single Molecule Spectroscopy ◽  
Circulating Nucleic Acids

Structural Dynamics of Nucleic Acids by Single-Molecule FRET

2013 ◽  
pp. 1-37 ◽  
Author(s):  
Asger Christian Krüger ◽  
Lasse Lava Hildebrandt ◽  
Sofie Louise Kragh ◽  
Victoria Birkedal
Keyword(s):  
Nucleic Acids ◽  
Structural Dynamics ◽  
Single Molecule ◽  
Single Molecule Fret

Structural dynamics of P-type ATPase ion pumps

2019 ◽  
Vol 47 (5) ◽  
pp. 1247-1257 ◽  
Author(s):  
Mateusz Dyla ◽  
Sara Basse Hansen ◽  
Poul Nissen ◽  
Magnus Kjaergaard
Keyword(s):  
Structural Dynamics ◽  
Single Molecule ◽  
New Technologies ◽  
Atp Hydrolysis ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Time Resolved ◽  
Dynamics Simulations ◽  
Types Of Information ◽  
P Type

Abstract P-type ATPases transport ions across biological membranes against concentration gradients and are essential for all cells. They use the energy from ATP hydrolysis to propel large intramolecular movements, which drive vectorial transport of ions. Tight coordination of the motions of the pump is required to couple the two spatially distant processes of ion binding and ATP hydrolysis. Here, we review our current understanding of the structural dynamics of P-type ATPases, focusing primarily on Ca2+ pumps. We integrate different types of information that report on structural dynamics, primarily time-resolved fluorescence experiments including single-molecule Förster resonance energy transfer and molecular dynamics simulations, and interpret them in the framework provided by the numerous crystal structures of sarco/endoplasmic reticulum Ca2+-ATPase. We discuss the challenges in characterizing the dynamics of membrane pumps, and the likely impact of new technologies on the field.

Monitoring the Structural Dynamics of U2 snRNA Via Single-Molecule Förster Resonance Energy Transfer

2018 ◽  
Author(s):  
Alexander Carl DeHaven
Keyword(s):  
Energy Transfer ◽  
Structural Dynamics ◽  
Single Molecule ◽  
Conformational Dynamics ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Förster Resonance Energy Transfer ◽  
U2 Snrna ◽  
Folding Dynamics ◽  
The Impact

This thesis contains four topic areas: a review of single-molecule microscropy methods and splicing, conformational dynamics of stem II of the U2 snRNA, the impact of post-transcriptional modifications on U2 snRNA folding dynamics, and preliminary findings on Mango aptamer folding dynamics.

Sign in / Sign up

Export Citation Format

Share Document