Effect of Drastic Sequence Alteration andd-Amino Acid Incorporation on the Membrane Binding Behavior of Lytic Peptides†

Biochemistry ◽  
2004 ◽  
Vol 43 (21) ◽  
pp. 6393-6403 ◽  
Author(s):  
Niv Papo ◽  
Yechiel Shai
Keyword(s):  
Amino Acid ◽  
Membrane Binding ◽  
Amino Acid Incorporation ◽  
Binding Behavior ◽  
Sequence Alteration ◽  
Acid Incorporation ◽  
Lytic Peptides

scholarly journals Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency

Amino Acids ◽  
2020 ◽  
Author(s):  
Thomas L. Williams ◽  
Debra J. Iskandar ◽  
Alexander R. Nödling ◽  
Yurong Tan ◽  
Louis Y. P. Luk ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Genetic Code ◽  
Unnatural Amino Acids ◽  
Trna Synthetase ◽  
Unnatural Amino Acid ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Genetic Code Expansion ◽  
Incorporation Efficiency

AbstractGenetic code expansion is a powerful technique for site-specific incorporation of an unnatural amino acid into a protein of interest. This technique relies on an orthogonal aminoacyl-tRNA synthetase/tRNA pair and has enabled incorporation of over 100 different unnatural amino acids into ribosomally synthesized proteins in cells. Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA from Methanosarcina species are arguably the most widely used orthogonal pair. Here, we investigated whether beneficial effect in unnatural amino acid incorporation caused by N-terminal mutations in PylRS of one species is transferable to PylRS of another species. It was shown that conserved mutations on the N-terminal domain of MmPylRS improved the unnatural amino acid incorporation efficiency up to five folds. As MbPylRS shares high sequence identity to MmPylRS, and the two homologs are often used interchangeably, we examined incorporation of five unnatural amino acids by four MbPylRS variants at two temperatures. Our results indicate that the beneficial N-terminal mutations in MmPylRS did not improve unnatural amino acid incorporation efficiency by MbPylRS. Knowledge from this work contributes to our understanding of PylRS homologs which are needed to improve the technique of genetic code expansion in the future.

scholarly journals FRUCTOSE-AMINO ACIDS IN LIVER: STIMULI OF AMINO ACID INCORPORATION IN VITRO

1955 ◽  
Vol 215 (1) ◽  
pp. 111-124 ◽  
Author(s):  
Henry Borsook ◽  
Adolph Abrams ◽  
Peter H. Lowy
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Incorporation ◽  
Acid Incorporation

scholarly journals INDEPENDENT ANTAGONISM OF AMINO ACID INCORPORATION INTO PROTEIN

1954 ◽  
Vol 210 (2) ◽  
pp. 837-849 ◽  
Author(s):  
M. Rabinovitz ◽  
Margaret E. Olson ◽  
David M. Greenberg
Keyword(s):  
Amino Acid ◽  
Amino Acid Incorporation ◽  
Acid Incorporation

scholarly journals The Involvement of Soluble Ribonucleic Acid in Amino Acid Incorporation by a Maize System

1964 ◽  
Vol 239 (6) ◽  
pp. 1762-1768 ◽  
Author(s):  
Rusty J. Mans ◽  
Caridad Maria Purcell ◽  
G. David Novelli
Keyword(s):  
Amino Acid ◽  
Ribonucleic Acid ◽  
Amino Acid Incorporation ◽  
Acid Incorporation
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