Ricin A-Chain Substrate Specificity in RNA, DNA, and Hybrid Stem−Loop Structures†

Biochemistry ◽  
2004 ◽  
Vol 43 (17) ◽  
pp. 4913-4922 ◽  
Author(s):  
Tim K. Amukele ◽  
Vern L. Schramm
Keyword(s):  
Substrate Specificity ◽  
Ricin A Chain ◽  
Stem Loop ◽  
A Chain ◽  
Ricin A

A hypothesis to explain the substrate reactivity of ribosomal and stem-loop RNA with ricin A-chain

Toxicon ◽  
1996 ◽  
Vol 34 (11-12) ◽  
pp. 1317-1324 ◽  
Author(s):  
Todd Link ◽  
Xiang Yang Chen ◽  
Lin Hao Niu ◽  
Vern L. Schramm
Keyword(s):  
Ricin A Chain ◽  
Stem Loop ◽  
Substrate Reactivity ◽  
A Chain ◽  
Ricin A

Ricin A-Chain:  Kinetics, Mechanism, and RNA Stem−Loop Inhibitors†

Biochemistry ◽  
1998 ◽  
Vol 37 (33) ◽  
pp. 11605-11613 ◽  
Author(s):  
Xiang-Yang Chen ◽  
Todd M. Link ◽  
Vern L. Schramm
Keyword(s):  
Ricin A Chain ◽  
Stem Loop ◽  
A Chain ◽  
Ricin A

scholarly journals The Png1–Rad23 complex regulates glycoprotein turnover

2006 ◽  
Vol 172 (2) ◽  
pp. 211-219 ◽  
Author(s):  
Ikjin Kim ◽  
Jungmi Ahn ◽  
Chang Liu ◽  
Kaori Tanabe ◽  
Jennifer Apodaca ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Substrate Specificity ◽  
Protein Degradation ◽  
Binding Proteins ◽  
Degradation Pathways ◽  
Ricin A Chain ◽  
Substrate Degradation ◽  
A Chain ◽  
Specific Degradation ◽  
Ricin A

Misfolded proteins in the endoplasmic reticulum (ER) are destroyed by a pathway termed ER-associated protein degradation (ERAD). Glycans are often removed from glycosylated ERAD substrates in the cytosol before substrate degradation, which maintains the efficiency of the proteasome. Png1, a deglycosylating enzyme, has long been suspected, but not proven, to be crucial in this process. We demonstrate that the efficient degradation of glycosylated ricin A chain requires the Png1–Rad23 complex, suggesting that this complex couples protein deglycosylation and degradation. Rad23 is a ubiquitin (Ub) binding protein involved in the transfer of ubiquitylated substrates to the proteasome. How Rad23 achieves its substrate specificity is unknown. We show that Rad23 binds various regulators of proteolysis to facilitate the degradation of distinct substrates. We propose that the substrate specificity of Rad23 and other Ub binding proteins is determined by their interactions with various cofactors involved in specific degradation pathways.

Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates†

Biochemistry ◽  
2005 ◽  
Vol 44 (11) ◽  
pp. 4416-4425 ◽  
Author(s):  
Tim K. Amukele ◽  
Setu Roday ◽  
Vern L. Schramm
Keyword(s):  
Ricin A Chain ◽  
Stem Loop ◽  
Dna Substrates ◽  
A Chain ◽  
Ricin A
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