Violaxanthin De-Epoxidase, the Xanthophyll Cycle Enzyme, Requires Lipid Inverted Hexagonal Structures for Its Activity†

Biochemistry ◽  
2004 ◽  
Vol 43 (15) ◽  
pp. 4417-4420 ◽  
Author(s):  
Dariusz Latowski ◽  
Hans-Erik Åkerlund ◽  
Kazimierz Strzałka
Keyword(s):  
Xanthophyll Cycle ◽  
Cycle Enzyme

Influence of ascorbate and pH on the activity of the diatom xanthophyll cycle-enzyme diadinoxanthin de-epoxidase

2006 ◽  
Vol 126 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Irina Grouneva ◽  
Torsten Jakob ◽  
Christian Wilhelm ◽  
Reimund Goss
Keyword(s):  
Xanthophyll Cycle ◽  
Cycle Enzyme

scholarly journals Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers

2002 ◽  
Vol 269 (18) ◽  
pp. 4656-4665 ◽  
Author(s):  
Dariusz Latowski ◽  
Jerzy Kruk ◽  
Kvetoslava Burda ◽  
Marta Skrzynecka-Jaskier ◽  
Anna Kostecka-Gugała ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Lipid Bilayers ◽  
Xanthophyll Cycle ◽  
Cycle Enzyme ◽  
Kinetics Of

PBA increases CFTR expression but at high doses inhibits Cl−secretion in Calu-3 airway epithelial cells

1999 ◽  
Vol 277 (4) ◽  
pp. L700-L708 ◽  
Author(s):  
Johannes Loffing ◽  
Bryan D. Moyer ◽  
Donna Reynolds ◽  
Bruce A. Stanton
Keyword(s):  
Protein Expression ◽  
Apical Membrane ◽  
Basolateral Membrane ◽  
Airway Epithelial Cells ◽  
Cell Phenotype ◽  
Apical Plasma Membrane ◽  
Airway Epithelial ◽  
High Concentration ◽  
Cycle Enzyme ◽  
Cf Transmembrane Conductance Regulator

Sodium 4-phenylbutyrate (PBA), a short-chain fatty acid, has been approved to treat patients with urea cycle enzyme deficiencies and is being evaluated in the management of sickle cell disease, thalassemia, cancer, and cystic fibrosis (CF). Because relatively little is known about the effects of PBA on the expression and function of the wild-type CF transmembrane conductance regulator (wt CFTR), the goal of this study was to examine the effects of PBA and related compounds on wt CFTR-mediated Cl−secretion. To this end, we studied Calu-3 cells, a human airway cell line that expresses endogenous wt CFTR and has a serous cell phenotype. We report that chronic treatment of Calu-3 cells with a high concentration (5 mM) of PBA, sodium butyrate, or sodium valproate but not of sodium acetate reduced basal and 8-(4-chlorophenylthio)-cAMP-stimulated Cl−secretion. Paradoxically, PBA enhanced CFTR protein expression 6- to 10-fold and increased the intensity of CFTR staining in the apical plasma membrane. PBA also increased protein expression of Na+-K+-ATPase. PBA reduced CFTR Cl−currents across the apical membrane but had no effect on Na+-K+-ATPase activity in the basolateral membrane. Thus a high concentration of PBA (5 mM) reduces Cl−secretion by inhibiting CFTR Cl−currents across the apical membrane. In contrast, lower therapeutic concentrations of PBA (0.05–2 mM) had no effect on cAMP-stimulated Cl−secretion across Calu-3 cells. We conclude that PBA concentrations in the therapeutic range are unlikely to have a negative effect on Cl−secretion. However, concentrations >5 mM might reduce transepithelial Cl−secretion by serous cells in submucosal glands in individuals expressing wt CFTR.

scholarly journals Xanthophyll-cycle dependence of the energy transfer between carotenoid dark states and chlorophylls in NPQ mutants of living plants and in LHC II

2008 ◽  
Vol 450 (4-6) ◽  
pp. 379-385 ◽  
Author(s):  
Stefan Bode ◽  
Claudia C. Quentmeier ◽  
Pen-Nan Liao ◽  
Tiago Barros ◽  
Peter J. Walla
Keyword(s):  
Energy Transfer ◽  
Xanthophyll Cycle ◽  
Lhc Ii ◽  
Cycle Dependence

Isocitrate lyase fromAspergillus nidulans: crystallization and X-ray analysis of a glyoxylate cycle enzyme

1997 ◽  
Vol 53 (4) ◽  
pp. 488-490 ◽  
Author(s):  
S. J. Langridge ◽  
P. J. Baker ◽  
J. R. De Lucas ◽  
S. E. Sedelnikova ◽  
G. Turner ◽  
...  
Keyword(s):  
Isocitrate Lyase ◽  
Glyoxylate Cycle ◽  
X Ray ◽  
Cycle Enzyme
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