Dynamic Characterization of a DNA Repair Enzyme:  NMR Studies of [methyl-13C]Methionine-Labeled DNA Polymerase β

Biochemistry ◽  
2004 ◽  
Vol 43 (28) ◽  
pp. 8911-8922 ◽  
Author(s):  
Bidisha Bose-Basu ◽  
Eugene F. DeRose ◽  
Thomas W. Kirby ◽  
Geoffrey A. Mueller ◽  
William A. Beard ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Polymerase ◽  
Dynamic Characterization ◽  
Dna Polymerase Β ◽  
Nmr Studies ◽  
Repair Enzyme

Detection and characterization of DNA polymerase activity in Toxoplasma gondii

Parasitology ◽  
1993 ◽  
Vol 107 (2) ◽  
pp. 135-139 ◽  
Author(s):  
A. Makioka ◽  
B. Stavros ◽  
J. T. Ellis ◽  
A. M. Johnson
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Sedimentation Coefficient ◽  
Polymerase Activity ◽  
Magnesium Ions ◽  
Dna Polymerase Β ◽  
Human Dna ◽  
Approximate Molecular Weight

SUMMARYA DNA polymerase activity has been detected and characterized in crude extracts from tachzoites of Toxoplasma gondii. The enzyme has a sedimentation coefficient of 6·4 S, corresponding to an approximate molecular weight of 150000 assuming a globular shape. Like mammalian DNA polymerase α, the DNA polymerase of T. gondii was sensitive to N-ethylmaleimide and inhibited by high ionic strength. However, the enzyme activity was not inhibited by aphidicolin which is an inhibitor of mammalian DNA polymerases α, δ and ε and also cytosine-β-D-arabinofuranoside-5′-triphosphate which is an inhibitor of α polymerase. The activity was inhibited by 2′,3′-dideoxythymidine-5′-triphosphate which is an inhibitor of mammalian DNA polymerase β and γ. Magnesium ions (Mg2+) were absolutely required for activity and its optimal concentration was 6 mM. The optimum potassium (K+) concentration was 50 mM and a higher concentration of K+ markedly inhibited the activity. Activity was optimal at pH 8. Monoclonal antibodies against human DNA polymerase did not bind to DNA polymerase of T. gondii. Thus the T. gondii enzyme differs from the human enzymes and may be a useful target for the design of toxoplasmacidal drugs.

scholarly journals Characterization of DNA polymerase β splicing variants in gastric cancer: The most frequent exon 2-deleted isoform is a non-coding RNA

2009 ◽  
Vol 670 (1-2) ◽  
pp. 79-87 ◽  
Author(s):  
Valeria Simonelli ◽  
Mariarosaria D’Errico ◽  
Domenico Palli ◽  
Rajendra Prasad ◽  
Samuel H. Wilson ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Dna Polymerase ◽  
Dna Polymerase Β ◽  
Exon 2 ◽  
Non Coding Rna ◽  
Splicing Variants

Functional characterization of the Caenorhabditis elegans DNA repair enzyme APN-1

DNA Repair ◽  
2012 ◽  
Vol 11 (10) ◽  
pp. 811-822 ◽  
Author(s):  
Xiaoming Yang ◽  
Jinjiang Fan ◽  
Alexander A. Ishchenko ◽  
Devang Patel ◽  
Murat K. Saparbaev ◽  
...  
Keyword(s):  
Dna Repair ◽  
Caenorhabditis Elegans ◽  
Functional Characterization ◽  
Repair Enzyme

Effects of DNA polymerase β gene over-expressed in transgenic Drosophila on DNA repair and recombination

1994 ◽  
Vol 69 (1) ◽  
pp. 21-33
Author(s):  
Mi-Ae YOO ◽  
Won-Ho LEE ◽  
Hye-Yeong HA ◽  
Jae-Ryeon RYU ◽  
Masamitsu YAMAGUCHI ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Polymerase ◽  
Dna Polymerase Β ◽  
Transgenic Drosophila

Characterization of the Tryptophan Fluorescence and Hydrodynamic Properties of Rat DNA Polymerase β

1994 ◽  
Vol 244 (2) ◽  
pp. 224-235 ◽  
Author(s):  
Soon-Jong Kim ◽  
Marc S. Lewis ◽  
Jay R. Knutson ◽  
Denise K. Porter ◽  
Amalendra Kumar ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Tryptophan Fluorescence ◽  
Dna Polymerase Β ◽  
Hydrodynamic Properties
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