Low Concentrations of Sodium Dodecyl Sulfate Induce the Extension of β2-Microglobulin-Related Amyloid Fibrils at a Neutral pH†

Biochemistry ◽  
2004 ◽  
Vol 43 (34) ◽  
pp. 11075-11082 ◽  
Author(s):  
Suguru Yamamoto ◽  
Kazuhiro Hasegawa ◽  
Itaru Yamaguchi ◽  
Shinobu Tsutsumi ◽  
József Kardos ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Amyloid Fibrils ◽  
Sodium Dodecyl ◽  
Β2 Microglobulin ◽  
Neutral Ph ◽  
Low Concentrations ◽  
Dodecyl Sulfate

scholarly journals Positive cooperative mechanistic binding of proteins at low concentrations: A comparison of poly (sodium N-undecanoyl sulfate) and sodium dodecyl sulfate

2011 ◽  
Vol 363 (2) ◽  
pp. 585-594 ◽  
Author(s):  
Susmita Das ◽  
Monica R. Sylvain ◽  
Vivian E. Fernand ◽  
Jack N. Losso ◽  
Bilal El-Zahab ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Low Concentrations ◽  
Dodecyl Sulfate

Sodium Dodecyl Sulfate Modified FeCo2O4with Enhanced Fenton-Like Activity at Neutral pH

2015 ◽  
Vol 119 (40) ◽  
pp. 23068-23074 ◽  
Author(s):  
Zichuan Ma ◽  
Limei Ren ◽  
Shengtao Xing ◽  
Yinsu Wu ◽  
Yuanzhe Gao
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Neutral Ph ◽  
Dodecyl Sulfate

Influence of Anionic Surfactants and Ionic Strength on α-Coymotrypsin-Catalyzed Reactions

1972 ◽  
Vol 50 (4) ◽  
pp. 392-398 ◽  
Author(s):  
J. E. Gaudin ◽  
T. Viswanatha
Keyword(s):  
Ionic Strength ◽  
Sodium Dodecyl Sulfate ◽  
Ethyl Ester ◽  
Sodium Dodecyl ◽  
Reaction Medium ◽  
Protein Ratio ◽  
Low Concentrations ◽  
Dodecyl Sulfate ◽  
Catalyzed Reactions ◽  
Hydrolysis Of

The effect of sodium dodecyl sulfate and sodium decylbenzene sulfonate on the chymotrypsin-catalyzed hydrolysis of N-acetyl-L-tyrosine ethyl ester was investigated. Pretreatment of the enzyme with sodium dodecyl sulfate produces inactivation which is dependent on the ratio of the surfactant to protein. Total loss of activity is achieved at a surfactant to protein (w/w) ratio of 15. Addition of chymotrypsin to a mixture of detergent and substrate does not affect the activity of the enzyme at low concentrations of sodium dodecyl sulfate. An enhancement in the activity is noted at higher surfactant concentrations, presumably due to the increase in the ionic strength of the reaction medium. The reaction of sodium decylbenzene sulfonate with chymotrypsin results in the formation of a series of complexes in an 'all or none' manner. Inactivation of the enzyme is noted initially at a surfactant to protein ratio of 1.0 and subsequently at ratios higher than 10. However, considerable activity is retained at surfactant to protein ratios of 3:5. The hydrolysis of N-acetyl-L-tyrosine ethyl ester is considerably influenced by the ionic strength of the medium. Increases in the ionic strength cause an enhancement in Vmax without affecting the affinity of the enzyme for the substrate.

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