Unnatural Amino Acid Mutagenesis:  A Precise Tool for Probing Protein Structure and Function†

Biochemistry ◽  
2004 ◽  
Vol 43 (37) ◽  
pp. 11623-11629 ◽  
Author(s):  
Pamela M. England
Keyword(s):  
Protein Structure ◽  
Amino Acid ◽  
Structure And Function ◽  
Unnatural Amino Acid ◽  
Protein Structure And Function ◽  
Unnatural Amino Acid Mutagenesis ◽  
Precise Tool ◽  
Amino Acid Mutagenesis ◽  
And Function

Expanding the genetic code of mammalian cells

2017 ◽  
Vol 45 (2) ◽  
pp. 555-562 ◽  
Author(s):  
James S. Italia ◽  
Yunan Zheng ◽  
Rachel E. Kelemen ◽  
Sarah B. Erickson ◽  
Partha S. Addy ◽  
...  
Keyword(s):  
Protein Structure ◽  
Amino Acid ◽  
Mammalian Cells ◽  
Living Cells ◽  
Structure And Function ◽  
Unnatural Amino Acid ◽  
Full Potential ◽  
Recent Developments ◽  
Small Footprint ◽  
And Function

In the last two decades, unnatural amino acid (UAA) mutagenesis has emerged as a powerful new method to probe and engineer protein structure and function. This technology enables precise incorporation of a rapidly expanding repertoire of UAAs into predefined sites of a target protein expressed in living cells. Owing to the small footprint of these genetically encoded UAAs and the large variety of enabling functionalities they offer, this technology has tremendous potential for deciphering the delicate and complex biology of the mammalian cells. Over the last few years, exciting progress has been made toward expanding the toolbox of genetically encoded UAAs in mammalian cells, improving the efficiency of their incorporation and developing innovative applications. Here, we provide our perspective on these recent developments and highlight the current challenges that must be overcome to realize the full potential of this technology.

The construction of an amino acid network for understanding protein structure and function

Amino Acids ◽  
2014 ◽  
Vol 46 (6) ◽  
pp. 1419-1439 ◽  
Author(s):  
Wenying Yan ◽  
Jianhong Zhou ◽  
Maomin Sun ◽  
Jiajia Chen ◽  
Guang Hu ◽  
...  
Keyword(s):  
Protein Structure ◽  
Amino Acid ◽  
Structure And Function ◽  
Protein Structure And Function ◽  
And Function

Order-disorder transitions of cytoplasmic N-termini in the mechanisms of P-type ATPases

2020 ◽  
Author(s):  
Khondker Rufaka Hossain ◽  
Daniel Clayton ◽  
Sophia C Goodchild ◽  
Alison Rodger ◽  
Richard James Payne ◽  
...  
Keyword(s):  
Protein Structure ◽  
Membrane Protein ◽  
Structure And Function ◽  
Membrane Protein Structure ◽  
Protein Structure And Function ◽  
Lipid Environment ◽  
Membrane Pumps ◽  
And Function ◽  
P Type

Membrane protein structure and function are modulated via interactions with their lipid environment. This is particularly true for the integral membrane pumps, the P-type ATPases. These ATPases play vital roles...

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