Mutagenesis Study of the 2Fe-2S Center and the FAD Binding Site of the Na+-Translocating NADH:Ubiquinone Oxidoreductase fromVibrio cholerae†

Biochemistry ◽  
2004 ◽  
Vol 43 (38) ◽  
pp. 12322-12330 ◽  
Author(s):  
Blanca Barquera ◽  
Mark J. Nilges ◽  
Joel E. Morgan ◽  
Leticia Ramirez-Silva ◽  
Weidong Zhou ◽  
...  
Keyword(s):  
Binding Site ◽  
Nadh:Ubiquinone Oxidoreductase ◽  
Mutagenesis Study ◽  
Fad Binding

Contribution of the FAD binding site residue tyrosine 308 to the stability of pea ferredoxin-NADP+ oxidoreductase

Biochemistry ◽  
1995 ◽  
Vol 34 (39) ◽  
pp. 12842-12848 ◽  
Author(s):  
Nora B. Calcaterra ◽  
Guillermo A. Pico ◽  
Elena G. Orellano ◽  
Jorgelina Ottado ◽  
Nestor Carrillo ◽  
...  
Keyword(s):  
Binding Site ◽  
The Stability ◽  
Fad Binding

scholarly journals Accessory NUMM (NDUFS6) subunit harbors a Zn-binding site and is essential for biogenesis of mitochondrial complex I

2015 ◽  
Vol 112 (18) ◽  
pp. 5685-5690 ◽  
Author(s):  
Katarzyna Kmita ◽  
Christophe Wirth ◽  
Judith Warnau ◽  
Sergio Guerrero-Castillo ◽  
Carola Hunte ◽  
...  
Keyword(s):  
Binding Site ◽  
Complex I ◽  
Proton Pumping ◽  
Nadh:Ubiquinone Oxidoreductase ◽  
X Ray Crystallography ◽  
Late Step ◽  
Multistep Process ◽  
Assembly Factor ◽  
Accessory Subunit ◽  
Assembly Intermediate

Mitochondrial proton-pumping NADH:ubiquinone oxidoreductase (respiratory complex I) comprises more than 40 polypeptides and contains eight canonical FeS clusters. The integration of subunits and insertion of cofactors into the nascent complex is a complicated multistep process that is aided by assembly factors. We show that the accessory NUMM subunit of complex I (human NDUFS6) harbors a Zn-binding site and resolve its position by X-ray crystallography. Chromosomal deletion of the NUMM gene or mutation of Zn-binding residues blocked a late step of complex I assembly. An accumulating assembly intermediate lacked accessory subunit N7BM (NDUFA12), whereas a paralog of this subunit, the assembly factor N7BML (NDUFAF2), was found firmly bound instead. EPR spectroscopic analysis and metal content determination after chromatographic purification of the assembly intermediate showed that NUMM is required for insertion or stabilization of FeS cluster N4.

FAD-binding site of glutathione reductase

1982 ◽  
Vol 160 (2) ◽  
pp. 287-308 ◽  
Author(s):  
G.E. Schulz ◽  
R.H. Schirmer ◽  
E.F. Pai
Keyword(s):  
Glutathione Reductase ◽  
Binding Site ◽  
Fad Binding

A Mutagenesis Study of the Putative Luciferin Binding Site Residues of Firefly Luciferase†

Biochemistry ◽  
2003 ◽  
Vol 42 (35) ◽  
pp. 10429-10436 ◽  
Author(s):  
Bruce R. Branchini ◽  
Tara L. Southworth ◽  
Martha H. Murtiashaw ◽  
Henrik Boije ◽  
Sarah E. Fleet
Keyword(s):  
Binding Site ◽  
Firefly Luciferase ◽  
Mutagenesis Study

scholarly journals The Ubiquinone-binding Site in NADH:Ubiquinone Oxidoreductase fromEscherichia coli

2003 ◽  
Vol 278 (28) ◽  
pp. 25731-25737 ◽  
Author(s):  
Xing Gong ◽  
Tong Xie ◽  
Linda Yu ◽  
Micaela Hesterberg ◽  
Dierk Scheide ◽  
...  
Keyword(s):  
Binding Site ◽  
Nadh:Ubiquinone Oxidoreductase ◽  
Fromescherichia Coli ◽  
Ubiquinone Binding
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