scholarly journals Expanding the Functional Repertoire of CTD Kinase I and RNA Polymerase II:  Novel PhosphoCTD-Associating Proteins in the Yeast Proteome†

Biochemistry ◽  
2004 ◽  
Vol 43 (50) ◽  
pp. 15702-15719 ◽  
Author(s):  
Hemali P. Phatnani ◽  
Janice C. Jones ◽  
Arno L. Greenleaf
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Yeast Proteome ◽  
Ctd Kinase I

scholarly journals Quantitation of the RNA Polymerase II Transcription Machinery in Yeast

2001 ◽  
Vol 276 (50) ◽  
pp. 47150-47153 ◽  
Author(s):  
Tilman Borggrefe ◽  
Ralph Davis ◽  
Avital Bareket-Samish ◽  
Roger D. Kornberg
Keyword(s):  
Quantitative Analysis ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Blot Analysis ◽  
Dot Blot ◽  
Yeast Proteome ◽  
Transcription Machinery ◽  
Large Complex ◽  
Dot Blot Analysis ◽  
Rna Polymerase Ii Transcription

TAP tags and dot blot analysis have been used to measure the amounts of RNA polymerase II transcription proteins in crude yeast extracts. The measurements showed comparable amounts of RNA polymerase II, TFIIE, and TFIIF, lower levels of TBP and TFIIB, and still lower levels of Mediator and TFIIH. These findings are consistent with the presumed roles of the transcription proteins, but do not support the idea of their recruitment in a single large complex to RNA polymerase II promoters. The approach employed here can be readily extended to quantitative analysis of the entire yeast proteome.

Nutrient-regulated gene expression in eukaryotes

2006 ◽  
Vol 73 ◽  
pp. 85-96 ◽  
Author(s):  
Richard J. Reece ◽  
Laila Beynon ◽  
Stacey Holden ◽  
Amanda D. Hughes ◽  
Karine Rébora ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcriptional Control ◽  
Direct Interaction ◽  
Signalling Pathways ◽  
A Cell ◽  
One Step ◽  
Higher Eukaryotes ◽  
Regulated Gene Expression

The recognition of changes in environmental conditions, and the ability to adapt to these changes, is essential for the viability of cells. There are numerous well characterized systems by which the presence or absence of an individual metabolite may be recognized by a cell. However, the recognition of a metabolite is just one step in a process that often results in changes in the expression of whole sets of genes required to respond to that metabolite. In higher eukaryotes, the signalling pathway between metabolite recognition and transcriptional control can be complex. Recent evidence from the relatively simple eukaryote yeast suggests that complex signalling pathways may be circumvented through the direct interaction between individual metabolites and regulators of RNA polymerase II-mediated transcription. Biochemical and structural analyses are beginning to unravel these elegant genetic control elements.

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