Proteolytic Activation of Protein Kinase Cα by Peroxynitrite in Stimulating Cytosolic Phospholipase A2in Pulmonary Endothelium:  Involvement of a Pertussis Toxin Sensitive Protein†

Biochemistry ◽  
2005 ◽  
Vol 44 (13) ◽  
pp. 5246-5257 ◽  
Author(s):  
Tapati Chakraborti ◽  
Sudip Das ◽  
Sajal Chakraborti
Keyword(s):  
Protein Kinase ◽  
Pertussis Toxin ◽  
Proteolytic Activation ◽  
Pulmonary Endothelium ◽  
Cytosolic Phospholipase ◽  
Protein Kinase Cα

scholarly journals Positive feedback of protein kinase C proteolytic activation during apoptosis

2002 ◽  
Vol 368 (3) ◽  
pp. 905-913 ◽  
Author(s):  
Sabrina LEVERRIER ◽  
Alice VALLENTIN ◽  
Dominique JOUBERT
Keyword(s):  
Pituitary Adenoma ◽  
Protein Kinase ◽  
Caspase 3 ◽  
Initial Step ◽  
Genotoxic Stress ◽  
Cytochrome C Release ◽  
Proteolytic Activation ◽  
Kinase C ◽  
Pkc Isoforms ◽  
Protein Kinase Cα

In contrast with protein kinase Cα (PKCα) and PKC∊, which are better known for promoting cell survival, PKCΔ is known for its pro-apoptotic function, which is exerted mainly through a caspase-3-dependent proteolytic activation pathway. In the present study, we used the rat GH3B6 pituitary adenoma cell line to show that PKCα and PKC∊ are activated and relocalized together with PKCΔ when apoptosis is induced by a genotoxic stress. Proteolytic activation is a crucial step used by the three isoforms since: (1) the catalytic domains of the PKCα, PKC∊ or PKCΔ isoforms (CDα, CD∊ and CDΔ respectively) accumulated, and this accumulation was dependent on the activity of both calpain and caspase; and (2) transient expression of CDα, CD∊ or CDΔ sufficed to induce apoptosis. However, following this initial step of proteolytic activation, the pathways diverge; cytochrome c release and caspase-3 activation are induced by CD∊ and CDΔ, but not by CDα. Another interesting finding of the present study is the proteolysis of PKCΔ induced by CD∊ expression that revealed the existence of a cross-talk between PKC isoforms during apoptosis. Hence the PKC family may participate in the apoptotic process of pituitary adenoma cells at two levels: downstream of caspase and calpain, and via retro-activation of caspase-3, resulting in the amplification of its own proteolytic activation.

Loss of gap junctions in biliary atresia occurs simultaneously with declined expression of Protein kinase Cα

2015 ◽  
Vol 53 (01) ◽  
Author(s):  
JHK Andruszkow ◽  
S Groos ◽  
C Klaus ◽  
U Schneider ◽  
C Petersen ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Gap Junctions ◽  
Biliary Atresia ◽  
Protein Kinase Cα

Transfection with Protein Kinase Cα Confers Increased Multidrug Resistance to MCF-7 Cells Expressing P-Glycoprotein

1991 ◽  
Vol 3 (6) ◽  
pp. 181-189 ◽  
Author(s):  
Gang Yu ◽  
Shakeel Ahmad ◽  
Angelo Aquino ◽  
Craig R. Fairchild ◽  
Jane B. Trepel ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Protein Kinase ◽  
P Glycoprotein ◽  
Protein Kinase Cα ◽  
Mcf 7
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